Alterações anatômicas causadas por Ralstonia solanacearum Smith e Pectobacterium carotovorum (Jones) em caules de Solanum tuberosum L.
Palavras-chave:
Histologia de caulina, infecção bacteriana, Papa
Resumo
A batata (Solanum tuberosum L.) é uma cultura notavelmente afetada por vários patógenos, incluindo bacterias, sendo importante estudar as alterações histológicas que eles produzem para entender os sintomas associados a cada doença. O objetivo desta pesquisa foi determinar as alterações anatômicas que ocorrem em caules de batata devido à infecção causada por Ralstonia solanacearum e Pectobacterium carotovorum. Foi realizado um ensaio com plantas da variedade Kennebec com 45 dias de idade, que foram infiltradas na base do caule principal com uma suspensão de 108 UFC.mL-1 de R. solanacearum ou P. carotovorum, e um tratamento foi incluído. controle em que as plantas foram tratadas com água destilada estéril. Doze dias após a inoculação, foram retirados segmentos do segundo entrenó do caule principal e fixados em FAA (formaldeído-ácido acético-etanol 70 %) até o processamento, para obtenção de cortes transversais do caule. Nas plantas inoculadas com R. solancaearum, observou-se a invasão dos vasos do xilema pela bactéria e a formação de tilos em algumas delas. Em relação ao P. carotovorum, foi encontrada necrose na forma de bandas descontínuas nas células da epiderme, córtex, cilindro vascular e medula, além de obstrução nos vasos do xilema pela bactéria e tilose em algumas delas. Los cambios anatómicos en el tallo, inducidos por las dos bacterias estudiadas, están directamente vinculados con la sintomatología de la enfermedad que cada una de ellas provoca en el cultivo de papa. Palavras chave: histologia de caulina, infecção bacteriana, papa.
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Referências
Agyemang, P. A., Niamul, M. D., Kersey, C. M., y Korsi, C. (2020). The bacterial soft rot pathogens, Pectobacterium carotovorum and P. atrosepticum, respond to different classes of virulence-inducing host chemical signals. Horticulturae, 6 (13), 1-13. https://doi.org/10.3390/horticulturae6010013
Artschwager, E. R. (1920). Pathological anatomy of potato blackleg. Journal of Agricultural Research, 20, 325-330. https://handle.nal.usda.gov/10113/IND43966302
Álvarez, B., Biosca E. G., y López, M. M. (2010). On the life of Ralstonia solanacearum, a destructive bacterial plant pathogen. En: Mendez-Vilas, A. (Ed.), Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology (pp. 267-279). Formatex Research Center. https://www.researchgate.net/publication/267772811
Barras, F., Van Gijsegem, F., y Chatterjee, A. K. (1994). Extracellular enzymes and pathogenesis of soft-rot Erwinia. Annual Review of Phytopathology, 32, 201–234. https://www.researchgate.net/publication/234838093
Buddenhagen, I. y Kelman, A. (1964). Biological and physiological aspect of bacterial wilt caused by Pseudomonas solanacearum. Annual Review of Phytopathology, 2, 203-230. https://doi.org/10.1146/annurev.py.02.090164.001223
Charkowski, A. O. (2015). Biology and control of Pectobacterium in potato. American Journal of Potato Research, 92, 223-229. https://doi.org/10.1007/s12230-015-9447-7
Charkowski, A. O. (2018). The changing face of bacterial soft-rot diseases. Annu. Rev. Phytopathol, 56, 269-288. https://doi.org/10.1146/annurev-phyto-080417- 045906
Charkowski A., Sharma, K., Parker, M. L., Secor, G. A., y Elphinstone, J. (2020). Bacterial diseases of potato. En: Campos, H. y Ortiz, O. (Eds.), The Potato Crop (pp. 351-388). Springer. https://doi.org/10.1007/978-3-030-28683-5_10
Czajkowski R., De Boer, W. J., Van Veen, J. A., y Van der Wolf, J. M. (2010). Downward vascular translocation of a green fluorescent protein-tagged strain of Dickeya sp. (Biovar 3) from stem and leaf inoculation sites on potato. Phytopathology, 100 (11), 1128–1137. https://doi.org/10.1094/PHYTO-03-10-0093
Czajkowski, R., Grabe, G. J., y Van der Wolf, J. M. (2009). Distribution of Dickeya spp. and Pectobacterium carotovorum subsp. carotovorum in naturally infected seed potatoes. Eur. J. Plant Pathol. 125, 263–275. https://doi.org/10.1007/s10658-009-9480-9
FAOSTAT Food and agriculture data. (2022) Database update on August https://www.fao.org/faostat/en/#data/QCL/visualize
Ferreira V., Pianzzola, M. J., Vilaró, F. L., Galván, G. A., Tondo, M. L., Rodriguez, M. V., Orellano, E. G., Valls, M., y Siri M. I. (2017). Interspecific potato breeding lines display differential colonization patterns and induced defense responses after Ralstonia solanacearum infection. Frontiers in Plant Science, 8, 1424. https://doi.org/10.3389/fpls.2017.01424
Fiers, M., Edel-Hermann, V., Chatot, C., Le Hingrat, Y., Alaboivette, C., y Steinberg, C. (2012). Potato soil-borne diseases. A review. Agronomy for Sustainable Development, 32, 93-132. https://doi.org/10.1007/s13593-011-0035-z
Genin, S., y Denny, T. P. (2012). Pathogenomics of the Ralstonia solanacearum species Complex. Annual Review of Phytopathology, 50(1), 67-89. https://doi.org/10.1146/annurev-phyto-081211-173000
Gayathiri, F., Bharathi B., y Priya K. (2018). Study of the enumeration of twelve clinical important bacterial populations at 0.5 McFarland Standard. International Journal of Creative Research Thoughts (IJCRT), 6(2), 880-893. https://www.ijcrt.org/papers/IJCRT1807341.pdf
Grimault, V., Gelie, B., Lemattre, M., Prior, P., y Schmit, J. (1994). Comparative histology of resistant and susceptible tomato cultivars infected by Pseudomonas solanacearum. Physiological and Molecular Plant Pathology 44, 105-123. https://doi.org/10.1016/S0885-5765(05)80105-5
Hayward, A. C. (1991). Biology and epidemiology of bacterial wilt caused by Pseudomonas solanacearum. Annual Review of Phytopathology, 29, 65– 87. https://doi.org/10.1146/annurev.py.29.090191.000433
Hernández, Y., Mariño, N., Trujillo, G., y Urbina, T. (2005) Invasión de Ralstonia solanacearum en tejidos de tallos de tomate. Revista de la Facultad de Agronomía (LUZ), 22, 181-190. http://ve.scielo.org/scielo.php?script=sci_arttext&pid=S0378-78182005000200008
Karim, Z., Hossain, M. S., y Begum, M. M. (2018). Ralstonia solanacearum: A threat to potato production in Bangladesh. Fundamental and Applied Agriculture 3(1), 407–421. https://doi.org/10.5455/faa.280361
Kashyap, A., Planas-Marqués, M., Capellades, M., Valls, M., y Coll, N. (2021). Blocking intruders: inducible physico-chemical barriers against plant vascular wilt pathogens. Journal of Experimental Botany, 72(2), 184–198. https://doi.org/10.1093/jxb/eraa444
Kastelein, P., Förch, M. G., Krijger, M. C., van der Zouwen, P.S., van den Berg, W., y van der Wolf, J.M. (2020). Systemic colonization of potato plants resulting from potato haulm inoculation with Dickeya solani or Pectobacterium parmentieri. Canadian Journal of Plant Pathology 43(1),1-15. https://doi.org/10.1080/07060661.2020.1777465
Khokhani D, Lowe-Power, T. M., Tran, T.M., y Allen, C. (2017). A single regulator mediates strategic switching between attachment/spread and growth/virulence in the plant pathogen Ralstonia solanacearum. American Society for Microbiology 8(5): 1-20. https://doi.org/10.1128/mBio.00895-17
Lowe-Power, T. M., Hendrich, C. G., von Roepenack-Lahaye, E., Li, B., Wu, D., Mitra, R., Dalsing, B. L., Ricca, P., Naidoo, J., Cook, D., Jancewicz, A., Masson, P., Thomma, B., Lahaye, T., Michael, A. J., y Allen, C. (2018a). Metabolomics of tomato xylem sap during bacterial wilt reveals Ralstonia solanacearum produces abundant putrescine, a metabolite that accelerates wilt disease. Environmental Microbiology, 20(4), 1330–1349. https://doi.org/10.1111/1462-2920.14020
Lowe-Power, T. M., Khokhani, D., y Allen, C. (2018b) How Ralstonia solanacearum exploits and thrives in the flowing plant xylem environment. Trends in Microbiology, 26(11), 929-942. https://www.researchgate.net/publication/325941527
Metcalfe, C., y Chalk, L. (1950). Anatomy of the dicotyledons (Vol. II). Clarendon Press. https://doi.org/10.1111/j.2042-7158.1950.tb13008.x
Moleleki, L. N., Pretorius, R. G., Tanui, C. K., Mosina, G., y Theron, J. (2017). A quorum sensing-defective mutant of Pectobacterium carotovorum ssp. brasiliense 1692 is attenuated in virulence and unable to occlude xylem tissue of susceptible potato plant stems. Molec. Plant Pathol., 18, 32–44. https://doi.org/10.1111/mpp.12372
Nakaho, K., Hibino, H., y Miyawa, H. (2000). Possible mechanisms movement of Ralstonia solanacearum in resistant tomato tissues. Journal of Phytopathology, 148(3), 181-190. https://doi.org/10.1046/j.1439-0434.2000.00476.x
Olivares, B. O., y Hernández, R. A. (2019). Sectorización ecoterritorial para la producción agrícola sostenible del cultivo de papa (Solanum tuberosum L.) en Carabobo, Venezuela. Ciencia y Tecnología Agropecuaria, 20 (2), 323-338. https://doi.org/10.21930/rcta.vol20_num2_art:1462
Pérombelon, M. C. (2002). Potato diseases caused by soft rot erwinias: an overview of pathogenesis. Plant Pathology, 51, 1–12. https://doi.org/10.1046/j.0032-0862.2001.Shorttitle.doc.x
Planas-Marquès, M., Kressin, J. P., Kashyap, A., Panthee, D., Louws, F. J., Coll, N. S., y Valls, M. (2020). Four bottlenecks restrict colonization and invasion by the pathogen Ralstonia solanacearum in resistant tomato, Journal of Experimental Botany, 71(6), 2157–2171. https://doi.org/10.1093/jxb/erz562
Shtein, I., Koyfman, A., Schwartz, A., Popper, Z., y Bar-On, B. (2020). Solanales stem biomechanical properties are primarily determined by morphology rather than internal structural anatomy and cell wall composition. Plants (Basel), 9(6), 678. https://doi.org/10.3390/plants9060678
Van der Wolf, J. M., y De Boer, S. (2007). Bacterial pathogens of potato. En: Vreugdenhil, D., Bradshaw, J., Gebhardt, C., Govers, F., MacKerron, D., Taylor, M., y Ross, H. (Eds.), Potato Biology and Biotechnology: Advances and Perspectives (pp. 596-617). Elsevier. http://base.dnsgb.com.ua/files/book/Agriculture/Cultures/Potato-Biology-and-Biotechnology.pdf
Xue, H., Lozano-Durán, R., y Macho, A. P. (2020). Insights into the root invasion by the plant pathogenic bacterium Ralstonia solanacearum. Plants, 9, 516. https://doi.org/10.3390/plants9040516
Yadeta K. A., y Thomma, J.B. (2013). The xylem as battleground for plant hosts and vascular wilt pathogens. Frontiers in Plant Science, 23(4), 97. https://doi.org/10.3389/fpls.2013.00097
Yuliar, Y., Nion, Y. A., y Toyota, K. (2015). Recent trends in control methods for bacterial wilt diseases caused by Ralstonia solanacearum. Microbes and Environments, 30, 1–11. https://doi.org/10.1264/jsme2.ME14144
Artschwager, E. R. (1920). Pathological anatomy of potato blackleg. Journal of Agricultural Research, 20, 325-330. https://handle.nal.usda.gov/10113/IND43966302
Álvarez, B., Biosca E. G., y López, M. M. (2010). On the life of Ralstonia solanacearum, a destructive bacterial plant pathogen. En: Mendez-Vilas, A. (Ed.), Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology (pp. 267-279). Formatex Research Center. https://www.researchgate.net/publication/267772811
Barras, F., Van Gijsegem, F., y Chatterjee, A. K. (1994). Extracellular enzymes and pathogenesis of soft-rot Erwinia. Annual Review of Phytopathology, 32, 201–234. https://www.researchgate.net/publication/234838093
Buddenhagen, I. y Kelman, A. (1964). Biological and physiological aspect of bacterial wilt caused by Pseudomonas solanacearum. Annual Review of Phytopathology, 2, 203-230. https://doi.org/10.1146/annurev.py.02.090164.001223
Charkowski, A. O. (2015). Biology and control of Pectobacterium in potato. American Journal of Potato Research, 92, 223-229. https://doi.org/10.1007/s12230-015-9447-7
Charkowski, A. O. (2018). The changing face of bacterial soft-rot diseases. Annu. Rev. Phytopathol, 56, 269-288. https://doi.org/10.1146/annurev-phyto-080417- 045906
Charkowski A., Sharma, K., Parker, M. L., Secor, G. A., y Elphinstone, J. (2020). Bacterial diseases of potato. En: Campos, H. y Ortiz, O. (Eds.), The Potato Crop (pp. 351-388). Springer. https://doi.org/10.1007/978-3-030-28683-5_10
Czajkowski R., De Boer, W. J., Van Veen, J. A., y Van der Wolf, J. M. (2010). Downward vascular translocation of a green fluorescent protein-tagged strain of Dickeya sp. (Biovar 3) from stem and leaf inoculation sites on potato. Phytopathology, 100 (11), 1128–1137. https://doi.org/10.1094/PHYTO-03-10-0093
Czajkowski, R., Grabe, G. J., y Van der Wolf, J. M. (2009). Distribution of Dickeya spp. and Pectobacterium carotovorum subsp. carotovorum in naturally infected seed potatoes. Eur. J. Plant Pathol. 125, 263–275. https://doi.org/10.1007/s10658-009-9480-9
FAOSTAT Food and agriculture data. (2022) Database update on August https://www.fao.org/faostat/en/#data/QCL/visualize
Ferreira V., Pianzzola, M. J., Vilaró, F. L., Galván, G. A., Tondo, M. L., Rodriguez, M. V., Orellano, E. G., Valls, M., y Siri M. I. (2017). Interspecific potato breeding lines display differential colonization patterns and induced defense responses after Ralstonia solanacearum infection. Frontiers in Plant Science, 8, 1424. https://doi.org/10.3389/fpls.2017.01424
Fiers, M., Edel-Hermann, V., Chatot, C., Le Hingrat, Y., Alaboivette, C., y Steinberg, C. (2012). Potato soil-borne diseases. A review. Agronomy for Sustainable Development, 32, 93-132. https://doi.org/10.1007/s13593-011-0035-z
Genin, S., y Denny, T. P. (2012). Pathogenomics of the Ralstonia solanacearum species Complex. Annual Review of Phytopathology, 50(1), 67-89. https://doi.org/10.1146/annurev-phyto-081211-173000
Gayathiri, F., Bharathi B., y Priya K. (2018). Study of the enumeration of twelve clinical important bacterial populations at 0.5 McFarland Standard. International Journal of Creative Research Thoughts (IJCRT), 6(2), 880-893. https://www.ijcrt.org/papers/IJCRT1807341.pdf
Grimault, V., Gelie, B., Lemattre, M., Prior, P., y Schmit, J. (1994). Comparative histology of resistant and susceptible tomato cultivars infected by Pseudomonas solanacearum. Physiological and Molecular Plant Pathology 44, 105-123. https://doi.org/10.1016/S0885-5765(05)80105-5
Hayward, A. C. (1991). Biology and epidemiology of bacterial wilt caused by Pseudomonas solanacearum. Annual Review of Phytopathology, 29, 65– 87. https://doi.org/10.1146/annurev.py.29.090191.000433
Hernández, Y., Mariño, N., Trujillo, G., y Urbina, T. (2005) Invasión de Ralstonia solanacearum en tejidos de tallos de tomate. Revista de la Facultad de Agronomía (LUZ), 22, 181-190. http://ve.scielo.org/scielo.php?script=sci_arttext&pid=S0378-78182005000200008
Karim, Z., Hossain, M. S., y Begum, M. M. (2018). Ralstonia solanacearum: A threat to potato production in Bangladesh. Fundamental and Applied Agriculture 3(1), 407–421. https://doi.org/10.5455/faa.280361
Kashyap, A., Planas-Marqués, M., Capellades, M., Valls, M., y Coll, N. (2021). Blocking intruders: inducible physico-chemical barriers against plant vascular wilt pathogens. Journal of Experimental Botany, 72(2), 184–198. https://doi.org/10.1093/jxb/eraa444
Kastelein, P., Förch, M. G., Krijger, M. C., van der Zouwen, P.S., van den Berg, W., y van der Wolf, J.M. (2020). Systemic colonization of potato plants resulting from potato haulm inoculation with Dickeya solani or Pectobacterium parmentieri. Canadian Journal of Plant Pathology 43(1),1-15. https://doi.org/10.1080/07060661.2020.1777465
Khokhani D, Lowe-Power, T. M., Tran, T.M., y Allen, C. (2017). A single regulator mediates strategic switching between attachment/spread and growth/virulence in the plant pathogen Ralstonia solanacearum. American Society for Microbiology 8(5): 1-20. https://doi.org/10.1128/mBio.00895-17
Lowe-Power, T. M., Hendrich, C. G., von Roepenack-Lahaye, E., Li, B., Wu, D., Mitra, R., Dalsing, B. L., Ricca, P., Naidoo, J., Cook, D., Jancewicz, A., Masson, P., Thomma, B., Lahaye, T., Michael, A. J., y Allen, C. (2018a). Metabolomics of tomato xylem sap during bacterial wilt reveals Ralstonia solanacearum produces abundant putrescine, a metabolite that accelerates wilt disease. Environmental Microbiology, 20(4), 1330–1349. https://doi.org/10.1111/1462-2920.14020
Lowe-Power, T. M., Khokhani, D., y Allen, C. (2018b) How Ralstonia solanacearum exploits and thrives in the flowing plant xylem environment. Trends in Microbiology, 26(11), 929-942. https://www.researchgate.net/publication/325941527
Metcalfe, C., y Chalk, L. (1950). Anatomy of the dicotyledons (Vol. II). Clarendon Press. https://doi.org/10.1111/j.2042-7158.1950.tb13008.x
Moleleki, L. N., Pretorius, R. G., Tanui, C. K., Mosina, G., y Theron, J. (2017). A quorum sensing-defective mutant of Pectobacterium carotovorum ssp. brasiliense 1692 is attenuated in virulence and unable to occlude xylem tissue of susceptible potato plant stems. Molec. Plant Pathol., 18, 32–44. https://doi.org/10.1111/mpp.12372
Nakaho, K., Hibino, H., y Miyawa, H. (2000). Possible mechanisms movement of Ralstonia solanacearum in resistant tomato tissues. Journal of Phytopathology, 148(3), 181-190. https://doi.org/10.1046/j.1439-0434.2000.00476.x
Olivares, B. O., y Hernández, R. A. (2019). Sectorización ecoterritorial para la producción agrícola sostenible del cultivo de papa (Solanum tuberosum L.) en Carabobo, Venezuela. Ciencia y Tecnología Agropecuaria, 20 (2), 323-338. https://doi.org/10.21930/rcta.vol20_num2_art:1462
Pérombelon, M. C. (2002). Potato diseases caused by soft rot erwinias: an overview of pathogenesis. Plant Pathology, 51, 1–12. https://doi.org/10.1046/j.0032-0862.2001.Shorttitle.doc.x
Planas-Marquès, M., Kressin, J. P., Kashyap, A., Panthee, D., Louws, F. J., Coll, N. S., y Valls, M. (2020). Four bottlenecks restrict colonization and invasion by the pathogen Ralstonia solanacearum in resistant tomato, Journal of Experimental Botany, 71(6), 2157–2171. https://doi.org/10.1093/jxb/erz562
Shtein, I., Koyfman, A., Schwartz, A., Popper, Z., y Bar-On, B. (2020). Solanales stem biomechanical properties are primarily determined by morphology rather than internal structural anatomy and cell wall composition. Plants (Basel), 9(6), 678. https://doi.org/10.3390/plants9060678
Van der Wolf, J. M., y De Boer, S. (2007). Bacterial pathogens of potato. En: Vreugdenhil, D., Bradshaw, J., Gebhardt, C., Govers, F., MacKerron, D., Taylor, M., y Ross, H. (Eds.), Potato Biology and Biotechnology: Advances and Perspectives (pp. 596-617). Elsevier. http://base.dnsgb.com.ua/files/book/Agriculture/Cultures/Potato-Biology-and-Biotechnology.pdf
Xue, H., Lozano-Durán, R., y Macho, A. P. (2020). Insights into the root invasion by the plant pathogenic bacterium Ralstonia solanacearum. Plants, 9, 516. https://doi.org/10.3390/plants9040516
Yadeta K. A., y Thomma, J.B. (2013). The xylem as battleground for plant hosts and vascular wilt pathogens. Frontiers in Plant Science, 23(4), 97. https://doi.org/10.3389/fpls.2013.00097
Yuliar, Y., Nion, Y. A., y Toyota, K. (2015). Recent trends in control methods for bacterial wilt diseases caused by Ralstonia solanacearum. Microbes and Environments, 30, 1–11. https://doi.org/10.1264/jsme2.ME14144
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2022-10-05
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Hernández, Y., García, M., & Mejías, R. (2022). Alterações anatômicas causadas por Ralstonia solanacearum Smith e Pectobacterium carotovorum (Jones) em caules de Solanum tuberosum L. Revista Da Faculdade De Agronomia Da Universidade De Zulia, 39(4), e223947. Obtido de https://www.produccioncientificaluz.org/index.php/agronomia/article/view/38890
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Direitos de Autor (c) 2022 Yonis Hernández, Marina García, Rafael Mejías
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