Cambios anatómicos provocados por Ralstonia solanacearum Smith y Pectobacterium carotovorum (Jones) en tallos de Solanum tuberosum L.
Palabras clave:
Histología caulinar, Infección bacteriana, Papa
Resumen
La papa (Solanum tuberosum L.) es un cultivo notablemente afectado por diversos patógenos, incluyendo los bacterianos, y es importante el estudio de las alteraciones histológicas que éstos producen para comprender los síntomas asociados a cada enfermedad. En esta investigación se determinaron los cambios anatómicos provocados por Ralstonia solanacearum y Pectobacterium carotovorum en tallos de Solanum tuberosum. Se realizó un ensayo con plantas de la variedad Kennebec de 45 días de edad, las cuales fueron infiltradas en la base del tallo principal con una suspensión de 108 UFC.mL-1 de R. solanacearum y P. carotovorum, incluyendo un tratamiento testigo en el que las plantas fueron tratadas con agua destilada estéril. Doce días después de la inoculación, se tomaron segmentos del segundo entrenudo del tallo principal y se fijaron en FAA (formaldehido-ácido acético-etanol 70 %) hasta su procesamiento para la obtención de secciones transversales del tallo. En las plantas inoculadas con R. solancaearum, se observó invasión en los vasos xilemáticos por la bacteria y formación de tílides en algunos de ellos. En cuanto a P. carotovorum, se evidenció necrosis en forma de bandas discontinuas en las células de la epidermis, corteza, cilindro vascular y médula, así como obstrucción en vasos del xilema y tilosis en algunos de ellos. Los cambios anatómicos en el tallo, inducidos por las dos bacterias estudiadas, están directamente vinculados con la sintomatología de la enfermedad que cada una de ellas provoca en el cultivo de papa.
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Agyemang, P. A., Niamul, M. D., Kersey, C. M., y Korsi, C. (2020). The bacterial soft rot pathogens, Pectobacterium carotovorum and P. atrosepticum, respond to different classes of virulence-inducing host chemical signals. Horticulturae, 6 (13), 1-13. https://doi.org/10.3390/horticulturae6010013
Artschwager, E. R. (1920). Pathological anatomy of potato blackleg. Journal of Agricultural Research, 20, 325-330. https://handle.nal.usda.gov/10113/IND43966302
Álvarez, B., Biosca E. G., y López, M. M. (2010). On the life of Ralstonia solanacearum, a destructive bacterial plant pathogen. En: Mendez-Vilas, A. (Ed.), Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology (pp. 267-279). Formatex Research Center. https://www.researchgate.net/publication/267772811
Barras, F., Van Gijsegem, F., y Chatterjee, A. K. (1994). Extracellular enzymes and pathogenesis of soft-rot Erwinia. Annual Review of Phytopathology, 32, 201–234. https://www.researchgate.net/publication/234838093
Buddenhagen, I. y Kelman, A. (1964). Biological and physiological aspect of bacterial wilt caused by Pseudomonas solanacearum. Annual Review of Phytopathology, 2, 203-230. https://doi.org/10.1146/annurev.py.02.090164.001223
Charkowski, A. O. (2015). Biology and control of Pectobacterium in potato. American Journal of Potato Research, 92, 223-229. https://doi.org/10.1007/s12230-015-9447-7
Charkowski, A. O. (2018). The changing face of bacterial soft-rot diseases. Annu. Rev. Phytopathol, 56, 269-288. https://doi.org/10.1146/annurev-phyto-080417- 045906
Charkowski A., Sharma, K., Parker, M. L., Secor, G. A., y Elphinstone, J. (2020). Bacterial diseases of potato. En: Campos, H. y Ortiz, O. (Eds.), The Potato Crop (pp. 351-388). Springer. https://doi.org/10.1007/978-3-030-28683-5_10
Czajkowski R., De Boer, W. J., Van Veen, J. A., y Van der Wolf, J. M. (2010). Downward vascular translocation of a green fluorescent protein-tagged strain of Dickeya sp. (Biovar 3) from stem and leaf inoculation sites on potato. Phytopathology, 100 (11), 1128–1137. https://doi.org/10.1094/PHYTO-03-10-0093
Czajkowski, R., Grabe, G. J., y Van der Wolf, J. M. (2009). Distribution of Dickeya spp. and Pectobacterium carotovorum subsp. carotovorum in naturally infected seed potatoes. Eur. J. Plant Pathol. 125, 263–275. https://doi.org/10.1007/s10658-009-9480-9
FAOSTAT Food and agriculture data. (2022) Database update on August https://www.fao.org/faostat/en/#data/QCL/visualize
Ferreira V., Pianzzola, M. J., Vilaró, F. L., Galván, G. A., Tondo, M. L., Rodriguez, M. V., Orellano, E. G., Valls, M., y Siri M. I. (2017). Interspecific potato breeding lines display differential colonization patterns and induced defense responses after Ralstonia solanacearum infection. Frontiers in Plant Science, 8, 1424. https://doi.org/10.3389/fpls.2017.01424
Fiers, M., Edel-Hermann, V., Chatot, C., Le Hingrat, Y., Alaboivette, C., y Steinberg, C. (2012). Potato soil-borne diseases. A review. Agronomy for Sustainable Development, 32, 93-132. https://doi.org/10.1007/s13593-011-0035-z
Genin, S., y Denny, T. P. (2012). Pathogenomics of the Ralstonia solanacearum species Complex. Annual Review of Phytopathology, 50(1), 67-89. https://doi.org/10.1146/annurev-phyto-081211-173000
Gayathiri, F., Bharathi B., y Priya K. (2018). Study of the enumeration of twelve clinical important bacterial populations at 0.5 McFarland Standard. International Journal of Creative Research Thoughts (IJCRT), 6(2), 880-893. https://www.ijcrt.org/papers/IJCRT1807341.pdf
Grimault, V., Gelie, B., Lemattre, M., Prior, P., y Schmit, J. (1994). Comparative histology of resistant and susceptible tomato cultivars infected by Pseudomonas solanacearum. Physiological and Molecular Plant Pathology 44, 105-123. https://doi.org/10.1016/S0885-5765(05)80105-5
Hayward, A. C. (1991). Biology and epidemiology of bacterial wilt caused by Pseudomonas solanacearum. Annual Review of Phytopathology, 29, 65– 87. https://doi.org/10.1146/annurev.py.29.090191.000433
Hernández, Y., Mariño, N., Trujillo, G., y Urbina, T. (2005) Invasión de Ralstonia solanacearum en tejidos de tallos de tomate. Revista de la Facultad de Agronomía (LUZ), 22, 181-190. http://ve.scielo.org/scielo.php?script=sci_arttext&pid=S0378-78182005000200008
Karim, Z., Hossain, M. S., y Begum, M. M. (2018). Ralstonia solanacearum: A threat to potato production in Bangladesh. Fundamental and Applied Agriculture 3(1), 407–421. https://doi.org/10.5455/faa.280361
Kashyap, A., Planas-Marqués, M., Capellades, M., Valls, M., y Coll, N. (2021). Blocking intruders: inducible physico-chemical barriers against plant vascular wilt pathogens. Journal of Experimental Botany, 72(2), 184–198. https://doi.org/10.1093/jxb/eraa444
Kastelein, P., Förch, M. G., Krijger, M. C., van der Zouwen, P.S., van den Berg, W., y van der Wolf, J.M. (2020). Systemic colonization of potato plants resulting from potato haulm inoculation with Dickeya solani or Pectobacterium parmentieri. Canadian Journal of Plant Pathology 43(1),1-15. https://doi.org/10.1080/07060661.2020.1777465
Khokhani D, Lowe-Power, T. M., Tran, T.M., y Allen, C. (2017). A single regulator mediates strategic switching between attachment/spread and growth/virulence in the plant pathogen Ralstonia solanacearum. American Society for Microbiology 8(5): 1-20. https://doi.org/10.1128/mBio.00895-17
Lowe-Power, T. M., Hendrich, C. G., von Roepenack-Lahaye, E., Li, B., Wu, D., Mitra, R., Dalsing, B. L., Ricca, P., Naidoo, J., Cook, D., Jancewicz, A., Masson, P., Thomma, B., Lahaye, T., Michael, A. J., y Allen, C. (2018a). Metabolomics of tomato xylem sap during bacterial wilt reveals Ralstonia solanacearum produces abundant putrescine, a metabolite that accelerates wilt disease. Environmental Microbiology, 20(4), 1330–1349. https://doi.org/10.1111/1462-2920.14020
Lowe-Power, T. M., Khokhani, D., y Allen, C. (2018b) How Ralstonia solanacearum exploits and thrives in the flowing plant xylem environment. Trends in Microbiology, 26(11), 929-942. https://www.researchgate.net/publication/325941527
Metcalfe, C., y Chalk, L. (1950). Anatomy of the dicotyledons (Vol. II). Clarendon Press. https://doi.org/10.1111/j.2042-7158.1950.tb13008.x
Moleleki, L. N., Pretorius, R. G., Tanui, C. K., Mosina, G., y Theron, J. (2017). A quorum sensing-defective mutant of Pectobacterium carotovorum ssp. brasiliense 1692 is attenuated in virulence and unable to occlude xylem tissue of susceptible potato plant stems. Molec. Plant Pathol., 18, 32–44. https://doi.org/10.1111/mpp.12372
Nakaho, K., Hibino, H., y Miyawa, H. (2000). Possible mechanisms movement of Ralstonia solanacearum in resistant tomato tissues. Journal of Phytopathology, 148(3), 181-190. https://doi.org/10.1046/j.1439-0434.2000.00476.x
Olivares, B. O., y Hernández, R. A. (2019). Sectorización ecoterritorial para la producción agrícola sostenible del cultivo de papa (Solanum tuberosum L.) en Carabobo, Venezuela. Ciencia y Tecnología Agropecuaria, 20 (2), 323-338. https://doi.org/10.21930/rcta.vol20_num2_art:1462
Pérombelon, M. C. (2002). Potato diseases caused by soft rot erwinias: an overview of pathogenesis. Plant Pathology, 51, 1–12. https://doi.org/10.1046/j.0032-0862.2001.Shorttitle.doc.x
Planas-Marquès, M., Kressin, J. P., Kashyap, A., Panthee, D., Louws, F. J., Coll, N. S., y Valls, M. (2020). Four bottlenecks restrict colonization and invasion by the pathogen Ralstonia solanacearum in resistant tomato, Journal of Experimental Botany, 71(6), 2157–2171. https://doi.org/10.1093/jxb/erz562
Shtein, I., Koyfman, A., Schwartz, A., Popper, Z., y Bar-On, B. (2020). Solanales stem biomechanical properties are primarily determined by morphology rather than internal structural anatomy and cell wall composition. Plants (Basel), 9(6), 678. https://doi.org/10.3390/plants9060678
Van der Wolf, J. M., y De Boer, S. (2007). Bacterial pathogens of potato. En: Vreugdenhil, D., Bradshaw, J., Gebhardt, C., Govers, F., MacKerron, D., Taylor, M., y Ross, H. (Eds.), Potato Biology and Biotechnology: Advances and Perspectives (pp. 596-617). Elsevier. http://base.dnsgb.com.ua/files/book/Agriculture/Cultures/Potato-Biology-and-Biotechnology.pdf
Xue, H., Lozano-Durán, R., y Macho, A. P. (2020). Insights into the root invasion by the plant pathogenic bacterium Ralstonia solanacearum. Plants, 9, 516. https://doi.org/10.3390/plants9040516
Yadeta K. A., y Thomma, J.B. (2013). The xylem as battleground for plant hosts and vascular wilt pathogens. Frontiers in Plant Science, 23(4), 97. https://doi.org/10.3389/fpls.2013.00097
Yuliar, Y., Nion, Y. A., y Toyota, K. (2015). Recent trends in control methods for bacterial wilt diseases caused by Ralstonia solanacearum. Microbes and Environments, 30, 1–11. https://doi.org/10.1264/jsme2.ME14144
Artschwager, E. R. (1920). Pathological anatomy of potato blackleg. Journal of Agricultural Research, 20, 325-330. https://handle.nal.usda.gov/10113/IND43966302
Álvarez, B., Biosca E. G., y López, M. M. (2010). On the life of Ralstonia solanacearum, a destructive bacterial plant pathogen. En: Mendez-Vilas, A. (Ed.), Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology (pp. 267-279). Formatex Research Center. https://www.researchgate.net/publication/267772811
Barras, F., Van Gijsegem, F., y Chatterjee, A. K. (1994). Extracellular enzymes and pathogenesis of soft-rot Erwinia. Annual Review of Phytopathology, 32, 201–234. https://www.researchgate.net/publication/234838093
Buddenhagen, I. y Kelman, A. (1964). Biological and physiological aspect of bacterial wilt caused by Pseudomonas solanacearum. Annual Review of Phytopathology, 2, 203-230. https://doi.org/10.1146/annurev.py.02.090164.001223
Charkowski, A. O. (2015). Biology and control of Pectobacterium in potato. American Journal of Potato Research, 92, 223-229. https://doi.org/10.1007/s12230-015-9447-7
Charkowski, A. O. (2018). The changing face of bacterial soft-rot diseases. Annu. Rev. Phytopathol, 56, 269-288. https://doi.org/10.1146/annurev-phyto-080417- 045906
Charkowski A., Sharma, K., Parker, M. L., Secor, G. A., y Elphinstone, J. (2020). Bacterial diseases of potato. En: Campos, H. y Ortiz, O. (Eds.), The Potato Crop (pp. 351-388). Springer. https://doi.org/10.1007/978-3-030-28683-5_10
Czajkowski R., De Boer, W. J., Van Veen, J. A., y Van der Wolf, J. M. (2010). Downward vascular translocation of a green fluorescent protein-tagged strain of Dickeya sp. (Biovar 3) from stem and leaf inoculation sites on potato. Phytopathology, 100 (11), 1128–1137. https://doi.org/10.1094/PHYTO-03-10-0093
Czajkowski, R., Grabe, G. J., y Van der Wolf, J. M. (2009). Distribution of Dickeya spp. and Pectobacterium carotovorum subsp. carotovorum in naturally infected seed potatoes. Eur. J. Plant Pathol. 125, 263–275. https://doi.org/10.1007/s10658-009-9480-9
FAOSTAT Food and agriculture data. (2022) Database update on August https://www.fao.org/faostat/en/#data/QCL/visualize
Ferreira V., Pianzzola, M. J., Vilaró, F. L., Galván, G. A., Tondo, M. L., Rodriguez, M. V., Orellano, E. G., Valls, M., y Siri M. I. (2017). Interspecific potato breeding lines display differential colonization patterns and induced defense responses after Ralstonia solanacearum infection. Frontiers in Plant Science, 8, 1424. https://doi.org/10.3389/fpls.2017.01424
Fiers, M., Edel-Hermann, V., Chatot, C., Le Hingrat, Y., Alaboivette, C., y Steinberg, C. (2012). Potato soil-borne diseases. A review. Agronomy for Sustainable Development, 32, 93-132. https://doi.org/10.1007/s13593-011-0035-z
Genin, S., y Denny, T. P. (2012). Pathogenomics of the Ralstonia solanacearum species Complex. Annual Review of Phytopathology, 50(1), 67-89. https://doi.org/10.1146/annurev-phyto-081211-173000
Gayathiri, F., Bharathi B., y Priya K. (2018). Study of the enumeration of twelve clinical important bacterial populations at 0.5 McFarland Standard. International Journal of Creative Research Thoughts (IJCRT), 6(2), 880-893. https://www.ijcrt.org/papers/IJCRT1807341.pdf
Grimault, V., Gelie, B., Lemattre, M., Prior, P., y Schmit, J. (1994). Comparative histology of resistant and susceptible tomato cultivars infected by Pseudomonas solanacearum. Physiological and Molecular Plant Pathology 44, 105-123. https://doi.org/10.1016/S0885-5765(05)80105-5
Hayward, A. C. (1991). Biology and epidemiology of bacterial wilt caused by Pseudomonas solanacearum. Annual Review of Phytopathology, 29, 65– 87. https://doi.org/10.1146/annurev.py.29.090191.000433
Hernández, Y., Mariño, N., Trujillo, G., y Urbina, T. (2005) Invasión de Ralstonia solanacearum en tejidos de tallos de tomate. Revista de la Facultad de Agronomía (LUZ), 22, 181-190. http://ve.scielo.org/scielo.php?script=sci_arttext&pid=S0378-78182005000200008
Karim, Z., Hossain, M. S., y Begum, M. M. (2018). Ralstonia solanacearum: A threat to potato production in Bangladesh. Fundamental and Applied Agriculture 3(1), 407–421. https://doi.org/10.5455/faa.280361
Kashyap, A., Planas-Marqués, M., Capellades, M., Valls, M., y Coll, N. (2021). Blocking intruders: inducible physico-chemical barriers against plant vascular wilt pathogens. Journal of Experimental Botany, 72(2), 184–198. https://doi.org/10.1093/jxb/eraa444
Kastelein, P., Förch, M. G., Krijger, M. C., van der Zouwen, P.S., van den Berg, W., y van der Wolf, J.M. (2020). Systemic colonization of potato plants resulting from potato haulm inoculation with Dickeya solani or Pectobacterium parmentieri. Canadian Journal of Plant Pathology 43(1),1-15. https://doi.org/10.1080/07060661.2020.1777465
Khokhani D, Lowe-Power, T. M., Tran, T.M., y Allen, C. (2017). A single regulator mediates strategic switching between attachment/spread and growth/virulence in the plant pathogen Ralstonia solanacearum. American Society for Microbiology 8(5): 1-20. https://doi.org/10.1128/mBio.00895-17
Lowe-Power, T. M., Hendrich, C. G., von Roepenack-Lahaye, E., Li, B., Wu, D., Mitra, R., Dalsing, B. L., Ricca, P., Naidoo, J., Cook, D., Jancewicz, A., Masson, P., Thomma, B., Lahaye, T., Michael, A. J., y Allen, C. (2018a). Metabolomics of tomato xylem sap during bacterial wilt reveals Ralstonia solanacearum produces abundant putrescine, a metabolite that accelerates wilt disease. Environmental Microbiology, 20(4), 1330–1349. https://doi.org/10.1111/1462-2920.14020
Lowe-Power, T. M., Khokhani, D., y Allen, C. (2018b) How Ralstonia solanacearum exploits and thrives in the flowing plant xylem environment. Trends in Microbiology, 26(11), 929-942. https://www.researchgate.net/publication/325941527
Metcalfe, C., y Chalk, L. (1950). Anatomy of the dicotyledons (Vol. II). Clarendon Press. https://doi.org/10.1111/j.2042-7158.1950.tb13008.x
Moleleki, L. N., Pretorius, R. G., Tanui, C. K., Mosina, G., y Theron, J. (2017). A quorum sensing-defective mutant of Pectobacterium carotovorum ssp. brasiliense 1692 is attenuated in virulence and unable to occlude xylem tissue of susceptible potato plant stems. Molec. Plant Pathol., 18, 32–44. https://doi.org/10.1111/mpp.12372
Nakaho, K., Hibino, H., y Miyawa, H. (2000). Possible mechanisms movement of Ralstonia solanacearum in resistant tomato tissues. Journal of Phytopathology, 148(3), 181-190. https://doi.org/10.1046/j.1439-0434.2000.00476.x
Olivares, B. O., y Hernández, R. A. (2019). Sectorización ecoterritorial para la producción agrícola sostenible del cultivo de papa (Solanum tuberosum L.) en Carabobo, Venezuela. Ciencia y Tecnología Agropecuaria, 20 (2), 323-338. https://doi.org/10.21930/rcta.vol20_num2_art:1462
Pérombelon, M. C. (2002). Potato diseases caused by soft rot erwinias: an overview of pathogenesis. Plant Pathology, 51, 1–12. https://doi.org/10.1046/j.0032-0862.2001.Shorttitle.doc.x
Planas-Marquès, M., Kressin, J. P., Kashyap, A., Panthee, D., Louws, F. J., Coll, N. S., y Valls, M. (2020). Four bottlenecks restrict colonization and invasion by the pathogen Ralstonia solanacearum in resistant tomato, Journal of Experimental Botany, 71(6), 2157–2171. https://doi.org/10.1093/jxb/erz562
Shtein, I., Koyfman, A., Schwartz, A., Popper, Z., y Bar-On, B. (2020). Solanales stem biomechanical properties are primarily determined by morphology rather than internal structural anatomy and cell wall composition. Plants (Basel), 9(6), 678. https://doi.org/10.3390/plants9060678
Van der Wolf, J. M., y De Boer, S. (2007). Bacterial pathogens of potato. En: Vreugdenhil, D., Bradshaw, J., Gebhardt, C., Govers, F., MacKerron, D., Taylor, M., y Ross, H. (Eds.), Potato Biology and Biotechnology: Advances and Perspectives (pp. 596-617). Elsevier. http://base.dnsgb.com.ua/files/book/Agriculture/Cultures/Potato-Biology-and-Biotechnology.pdf
Xue, H., Lozano-Durán, R., y Macho, A. P. (2020). Insights into the root invasion by the plant pathogenic bacterium Ralstonia solanacearum. Plants, 9, 516. https://doi.org/10.3390/plants9040516
Yadeta K. A., y Thomma, J.B. (2013). The xylem as battleground for plant hosts and vascular wilt pathogens. Frontiers in Plant Science, 23(4), 97. https://doi.org/10.3389/fpls.2013.00097
Yuliar, Y., Nion, Y. A., y Toyota, K. (2015). Recent trends in control methods for bacterial wilt diseases caused by Ralstonia solanacearum. Microbes and Environments, 30, 1–11. https://doi.org/10.1264/jsme2.ME14144
Publicado
2022-10-05
Cómo citar
Hernández, Y., García, M., & Mejías, R. (2022). Cambios anatómicos provocados por Ralstonia solanacearum Smith y Pectobacterium carotovorum (Jones) en tallos de Solanum tuberosum L. Revista De La Facultad De Agronomía De La Universidad Del Zulia, 39(4), e223947. Recuperado a partir de https://www.produccioncientificaluz.org/index.php/agronomia/article/view/38890
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Producción Vegetal
Derechos de autor 2022 Yonis Hernández, Marina García, Rafael Mejías
Esta obra está bajo licencia internacional Creative Commons Reconocimiento-NoComercial-CompartirIgual 4.0.
