Identificación y caracterización morfológica de actinomicetos marinos como agentes de biocontrol de Fusarium solani en tomate
Palabras clave:
Streptomyces, Nocardiopsis, antagonismo, Solanum lycopersicum
Resumen
Fusarium spp. daña a las raíces de los cultivos, su control es con fungicidas sintéticos, sin embargo, los actinomicetos marinos puede ser una opción al uso de agroquímicos. El objetivo de este trabajo fue la identificación y caracterización morfológica de actinomicetos marinos como antagonistas a Fusarium solani (Mart.) Sacc. Fusarium spp. fue aislado de plantas enfermas de tomate y los actinomicetos de sedimento de manglares, ambos se identificaron a través de claves taxonómicas y por técnicas moleculares. Se obtuvieron ocho aislamientos de Fusarium spp., siendo H8 el más virulento y fue identificado como F. solani. Se aislaron 30 actinomicetos, de los cuales solo cuatro inhibieron al fitopatógeno, siendo A19 el que inhibió en un 70% al hongo y fue identificado como Streptomyces sp. Los actinomicetos marinos pueden ser una opción para el manejo de enfermedades en plantas de interés agrícola.
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Gopalakrishnan, S., Srinivas, V., Naresh, N., Pratyusha, S., Ankati, S., Madhuprakash, J., Govindaraj, M. and Sharma, R. (2021). Deciphering the antagonistic effect of Streptomyces spp. and host-plant resistance induction against charcoal rot of sorghum. Planta, 253(2), 1-12. https://doi.org/10.1007/s00425-021-03577-5
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Azadeh, B. F., Sariah, M. and Wong, M. Y. (2010). Characterization of Burkholderia cepacia genomovar I as a potential biocontrol agent of Ganoderma boninense in oil palm. African Journal of Biotechnology, 9(24), 3542-3548.
Barka, E. A., Vatsa, P., Sanchez, L., Gaveau-Vaillant, N., Jacquard, C., Klenk, H. P., Clément, C., Ouhdouch, Y. and van Wezel, G. P. (2016). Taxonomy, physiology, and natural products of Actinobacteria. Microbiology and Molecular Biology Reviews: MMBR, 80(1), 1-43. https://doi.org/10.1128/MMBR.00019-15
Benhadj, M., Gacemi-Kirane, D., Menasria, T., Guebla, K. and Ahmane, Z. (2019). Screening of rare actinomycetes isolated from natural wetland ecosystem (Fetzara Lake, northeastern Algeria) for hydrolytic enzymes and antimicrobial activities. Journal of King Saud University-Science, 31(4), 706-712. https://doi.org/10.1016/j.jksus.2018.03.008
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Choi, H. W., Hong, S. K., Lee, Y. K., Kim, W. G. and Chun, S. (2018). Taxonomy of Fusarium fujikuroi species complex associated with bakanae on rice in Korea. Australasian Plant Pathology, 47(1), 23-34. https://doi.org/10.1007/s13313-017-0536-6
Clarridge, J. E. (2004). Impact of 16S rRNA gene sequence analysis for identification of bacteria on clinical microbiology and infectious diseases. Clinical Microbiology Reviews, 17(4), 840-862. https://doi.org/10.1128/CMR.17.4.840-862.2004
Duarte Leal, Y., Echevarría Hernández, A. y Martínez Coca, B. (2016). Identificación y caracterización de aislamientos de Fusarium spp. presentes en garbanzo (Cicer arietinum L.) en Cuba. Revista de Protección Vegetal, 31(3), 173-183. https://cutt.ly/qnva8A5
Duraipandiyan, V., Sasi, A. H., Islam, V. I. H., Valanarasu, M. and Ignacimuthu, S. (2010). Antimicrobial properties of actinomycetes from the soil of Himalaya. Journal de Mycologie Medicale, 20(1), 15-20. https://doi.org/10.1016/j.mycmed.2009.11.002
Gadhave, A. D., Patil, P. D., Dawale, M. B., Suryawnshi, A. P., Joshi, M. S. and Giri, V. V. (2020). In-vitro Evaluation of different fungicides and bioagents against Fusarium oxysporum f. sp. lycopersici. International Journal of Current Microbiology and Applied Sciences, 9(8), 3576-3584. https://doi.org/10.20546/ijcmas.2020.908.412
Gong, B., Chen, S., Lan, W., Huang, Y. and Zhu, X. (2018). Antibacterial and antitumor potential of actinomycetes isolated from mangrove soil in the Maowei Sea of the southern coast of China. Iranian Journal of Pharmaceutical Research, 17(4), 1339-1346. https://doi.org/10.22037/ijpr.2018.2280
Gopalakrishnan, S., Srinivas, V., Naresh, N., Pratyusha, S., Ankati, S., Madhuprakash, J., Govindaraj, M. and Sharma, R. (2021). Deciphering the antagonistic effect of Streptomyces spp. and host-plant resistance induction against charcoal rot of sorghum. Planta, 253(2), 1-12. https://doi.org/10.1007/s00425-021-03577-5
Goudjal, Y., Toumatia, O., Yekkour, A., Sabaou, N., Mathieu, F. and Zitouni, A. (2014). Biocontrol of Rhizoctonia solani damping-off and promotion of tomato plant growth by endophytic actinomycetes isolated from native plants of Algerian Sahara. Microbiological Research, 169(1), 59-65. https://doi.org/10.1016/j.micres.2013.06.014
Igarashi, M., Sawa, R., Umekita, M., Hatano, M., Arisaka, R., Hayashi, C., Ishizaki, Y., Suzuki, M. and Kato, C. (2021). Sealutomicins, new enediyne antibiotics from the deep-sea actinomycete Nonomuraea sp. MM565M-173N2. Journal of Antibiotics, 74, 291-299. https://doi.org/10.1038/s41429-020-00402-1
Intra, B., Mungsuntisuk, I., Nihira, T., Igarashi, Y. and Panbangred, W. (2011). Identifcation of actinomycetes from plant rhizospheric soils with inhibitory activity against Colletotrichum spp., the causative agent of anthracnose disease. BMC Research Notes, 4, 98. https://doi.org/10.1186/1756-0500-4-98
Kumar, S., Stecher, G., Li, M., Knyaz, C. and Tamura, K. (2018). MEGA X: Molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution, 35(6), 1547-1549. https://doi.org/10.1093/molbev/msy096
Li, Y. T., Hwang, S. G., Huang, Y. M. and Huang, C. H. (2018). Effects of Trichoderma asperellum on nutrient uptake and Fusarium wilt of tomato. Crop Protection, 110, 275-282. https://doi.org/10.1016/j.cropro.2017.03.021
Ling, L., Han, X., Li, X., Zhang, X., Wang, H., Zhang, L., Cao, P., Wu, Y., Wang, X., Zhao, J. and Xiang, W. (2020). A Streptomyces sp. NEAU-HV9: Isolation, identification, and potential as a biocontrol agent against Ralstonia solanacearum of tomato plants. Microorganisms, 8(3), 351. https://doi.org/10.3390/microorganisms8030351
Maluin, F. N. and Hussein, M. Z. (2020). Chitosan-based agronanochemicals as a sustainable alternative in crop protection. Molecules, 25(7), 1611. https://doi.org/10.3390/molecules25071611
Marlatt, M. L., Correll, J. C., Kaufmann, P. and Cooper, P. E. (1996). Two genetically distinct populations of Fusarium oxysporum f. sp. lycopersici race 3 in the United States. Plant Disease, 80(12), 1336-1342. https://doi.org/10.1094/PD-80-1336
Murugan, L., Krishnan, N., Venkataravanappa, V., Saha, S., Mishra, A. K., Sharma, B. K. and Rai, A. B. (2020). Molecular characterization and race identification of Fusarium oxysporum f. sp. lycopersici infecting tomato in India. 3 Biotech, 10, 486. https://doi.org/10.1007/s13205-020-02475-z
Nirmaladevi, D., Venkataramana, M., Srivastava, R. K., Uppalapati, S. R., Gupta, V. K., Yli-Mattila, T., Tsui, K. M., Srinivas, C., Niranjana, S. R. and Chandra, N. S. (2016). Molecular phylogeny, pathogenicity and toxigenicity of Fusarium oxysporum f. sp. lycopersici. Scientific Reports, 6(1), 1-14. https://doi.org/10.1038/srep21367
Ochoa, J. L., Hernández-Montiel, L. G., Latisnere-Barragán, H., León de La Luz, J. L. and Larralde-Corona, C. P. (2007). Isolation and identification of pathogenic fungi from orange Citrus sinensis L. Osbeck cultured in Baja California Sur, México. Ciencia y Tecnología Alimentaria, 5(5), 352-359. https://doi.org/10.1080/11358120709487712
Okubara, P. A., Schroeder, K. L. and Paulitz, T. C. (2005). Real-time polymerase chain reaction: applications to studies on soilborne pathogens. Canadian Journal of Plant Pathology, 27(3), 300-313. https://doi.org/10.1080/07060660509507229
Palla, M. S., Guntuku, G. S., Muthyala, M. K. K., Pingali, S. and Sahu, P. K. (2018). Isolation and molecular characterization of antifungal metabolite producing actinomycete from mangrove soil. Beni-Suef University Journal of Basic and Applied Sciences, 7(2), 250-256. https://doi.org/10.1016/j.bjbas.2018.02.006
Rathore, D. S., Sheikh, M., Gohel, S. and Singh, S. P. (2019). Isolation strategies, abundance and characteristics of the marine actinomycetes of Kachhighadi, Gujarat, India. Journal of the Marine Biological Association of India, 61(1), 71-78. http://dx.doi.org/10.6024/jmbai.2019.61.1.2028-11
Reyes-Pérez, J. J., Luna-Murillo, R. A., Reyes-Bermeo, M. R., Vázquez-Morán, V. F., Zambrano-Burgos, D. y Torres-Rodríguez, J. A. (2018). Efecto de los abonos orgánicos sobre la respuesta productiva del tomate (Solanum lycopersicum L). Revista de la Facultad de Agronomía de la Universidad del Zulia, 35(1), 26-39. https://cutt.ly/bnvsmtW
Roncero, M. I., Di Pietro, A., Ruiz-Roldán, M. C., Huertas-González, M. D., Garcia-Maceira, F. I., Méglecz, E., Jiménez, A., Caracuel, Z., Sancho-Zapatero, R., Hera, C., Gómez-Gómez, E., Ruiz-Rubio, M., González-Verdejo, C. I. and Páez, M. J. (2000). Role of cell wall-degrading enzymes in pathogenicity of Fusarium oxysporum. Revista Iberoamericana de Micología, 17(1), S47-53.
Sangkanu, S., Rukachaisirikul, V., Suriyachadkun, C. and Phongpaichit, S. (2017). Evaluation of antibacterial potential of mangrove sediment-derived actinomycetes. Microbial Pathogenesis, 112, 303-312. https://doi.org/10.1016/j.micpath.2017.10.010
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Publicado
2022-02-13
Cómo citar
Torres-Rodriguez, J., Reyes-Pérez, J., Castellanos, T., Angulo, C., Quiñones-Aguilar, E., & Hernandez-Montiel, L. (2022). Identificación y caracterización morfológica de actinomicetos marinos como agentes de biocontrol de Fusarium solani en tomate. Revista De La Facultad De Agronomía De La Universidad Del Zulia, 39(1), e223915. Recuperado a partir de https://www.produccioncientificaluz.org/index.php/agronomia/article/view/37706
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Sección
Producción Vegetal
Derechos de autor 2022 Juan Antonio Torres-Rodriguez, Juan José Reyes-Pérez, Thelma Castellanos, Carlos Angulo, Evangelina Esmeralda Quiñones-Aguilar y Luis Guillermo Hernandez-Montiel
Esta obra está bajo licencia internacional Creative Commons Reconocimiento-NoComercial-CompartirIgual 4.0.
