Contributions and application advances of japan in the development of rice genetic transformation technology systems
Keywords:
Japan, rice, transgenic technology, Agrobacterium-mediated transformation, genetic transformation system
Abstract
Based on a systematic literature retrieval from multiple mainstream databases including Web of Science, Google Scholar and J-STAGE, this paper collected and sorted relevant research literature published from 1960 to 2024, and also supplemented information through exchanges with Japanese rice biotechnology researchers. Japan has made foundational contributions to rice genetic transformation technology, establishing key systems such as protoplast regeneration and Agrobacterium-mediated transformation. These advances have enabled the development of transgenic rice with improved stress resistance, enhanced quality, and nutritional fortification. Despite slow commercialization domestically, Japan's technological platforms have become essential tools for global functional genomics and molecular breeding in rice. The integration of these systems with gene editing promises innovative solutions for future food security challenges.
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References
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Hiei, Y., Ohta, S., Komari, T., & Kumashiro, T. (1994). Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA. The Plant Journal, 6(2), 271-282. https://doi.org/10.1046/j.1365-313x.1994.6020271.x
Isshiki, M., Morino, K., Nakajima, M., Okagaki, R. J., Wessler, S. R., Izawa, T., & Shimamoto, K. (1998). A naturally occurring functional allele of the rice waxy locus has a GT to TT mutation at the 5' splice site of the first intron. The Plant Journal, 15(1), 133-138. https://doi.org/10.1046/j.1365-313x.1998.00189.x
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Katsube, T., Kurisaka, N., Ogawa, M., Maruyama, N., Ohtsuka, R., Utsumi, S., & Takaiwa, F. (1999). Accumulation of soybean glycinin and its assembly with the glutelins in rice. Plant Physiology, 120(4), 1063-1074. https://doi.org/10.1104/pp.120.4.1063
Kawahigashi, H., Hirose, S., Ohkawa, H., & Ohkawa, Y. (2003). Transgenic rice plants expressing human CYP1A1 exude herbicide metabolites from their roots. Plant Science, 165(2), 373-381. https://doi.org/10.1016/S0168-9452(03)00197-3
Komamine, A. (2003). My way with plant cell cultures: significance of experimental systems in plant biology. In Vitro Cellular & Developmental Biology - Plant, 39(2), 63. https://doi.org/10.1079/IVP2002404
Komari, T., Takakura, Y., Ueki, J., Kato, N., Ishida, Y., & Hiei, Y. (2006). Binary vectors and super-binary vectors. Methods in Molecular Biology, 343, 15-42. https://doi.org/10.1385/1-59745-130-4:15
Kyozuka, J., Hayashi, Y., & Shimamoto, K. (1987). High frequency plant regeneration from rice protoplasts by novel nurse culture methods. Molecular and General Genetics MGG, 206(3), 408-413. https://doi.org/10.1007/BF00428879
Li, D., Nanseki, T., Chomei, Y., & Kuang, J. (2023). A review of smart agriculture and production practices in Japanese large-scale rice farming. Journal of the Science of Food and Agriculture, 103(4), 1609-1620. https://doi.org/10.1002/jsfa.12204
Masuda, H., Kobayashi, T., Ishimaru, Y., Takahashi, M., Aung, M. S., Nakanishi, H., Mori, S., & Nishizawa, N. K. (2013). Iron-biofortification in rice by the introduction of three barley genes participated in mugineic acid biosynthesis with soybean ferritin gene. Frontiers in Plant Science, 4, 132. https://doi.org/10.3389/fpls.2013.00132
Matsumura, T., Tabayashi, N., Kamagata, Y., Souma, C., & Saruyama, H. (2002). Wheat catalase expressed in transgenic rice can improve tolerance against low temperature stress. Physiologia Plantarum, 116(3), 317-327. https://doi.org/10.1034/j.1399-3054.2002.1160306.x
Matsuoka, M., Furbank, R. T., Fukayama, H., & Miyao, M. (2001). Molecular Engineering of C4 Photosynthesis. Annual Review of Plant Physiology and Plant Molecular Biology, 52(1), 297-314. https://doi.org/10.1146/annurev.arplant.52.1.297
Matsushita, J., Otani, M., Wakita, Y., Tanaka, O., & Shimada, T. (1999). Transgenic plant regeneration through silicon carbide whisker-mediated transformation of rice (Oryza sativa L.). Breeding Science, 49(1), 21-26. https://doi.org/10.1270/jsbbs.49.21
Nakagahra, M., Okuno, K., & Vaughan, D. (1997). Rice genetic resources: history, conservation, investigative characterization and use in Japan. Plant Molecular Biology, 35(1-2), 69-77. https://doi.org/10.1023/A:1005784431759
Nakase, M., Aoki, N., Matsuda, T., & Adachi, T. (1997). Characterization of a novel rice bZIP protein which binds to the alpha-globulin promoter. Plant Molecular Biology, 33(3), 513-522. https://doi.org/10.1023/a:1005784717782
Niizeki, H., & Oono, K. (1968). Induction of haploid rice plant from anther culture. Proceedings of the Japan Academy, 44(6), 554-557. https://doi.org/10.2183/pjab1945.44.554
Ozawa, K. (2012). A high-efficiency Agrobacterium-mediated transformation system of rice (Oryza sativa L.). In Transgenic Plants: Methods and Protocols (pp. 51-57). Humana Press. https://doi.org/10.1007/978-1-61779-558-9_5
Ozawa, K., & Komamine, A. (1989). Establishment of a system of high-frequency embryogenesis from long-term cell suspension cultures of rice (Oryza sativa L.). Theoretical and Applied Genetics, 77(2), 205-211. https://doi.org/10.1007/BF00266188
Radi, S. H., & Maeda, E. (1987). Ultrastructures of rice scutellum cultured with attached root using two separate media as compared to the intact seedlings. Japanese Journal of Crop Science, 56(1), 73-84. https://doi.org/10.1626/jcs.56.73
Satoh, K., Kondoh, H., Sasaya, T., Shimizu, T., Choi, I. R., Omura, T., & Kikuchi, S. (2010). Selective modification of rice (Oryza sativa) gene expression by rice stripe virus infection. Journal of General Virology, 91(Pt 1), 294-305. https://doi.org/10.1099/vir.0.015990-0
Sharoni, A. M., Nuruzzaman, M., Satoh, K., Shimizu, T., Kondoh, H., Sasaya, T., Choi, I. R., Omura, T., & Kikuchi, S. (2011). Gene structures, classification and expression models of the AP2/EREBP transcription factor family in rice. Plant and Cell Physiology, 52(2), 344-360. https://doi.org/10.1093/pcp/pcq196
Shimamoto, K., Terada, R., Izawa, T., & Fujimoto, H. (1989). Fertile transgenic rice plants regenerated from transformed protoplasts. Nature, 338(6212), 274-276. https://doi.org/10.1038/338274a0
Sukegawa, S., Nureki, O., Toki, S., & Saika, H. (2023). Genome editing in rice mediated by miniature size Cas nuclease SpCas12f. Frontiers in Genome Editing, 5, 1138843. https://doi.org/10.3389/fgeed.2023.1138843
Sun, Z., Sun, L., & Shu, L. (1991). Utilization of somaclonal variation in rice breeding. In Rice Biotechnology (pp. 328-346). CAB International.
Suzuki, S., Murai, N., Burnell, J. N., & Arai, M. (2000). Changes in photosynthetic carbon flow in transgenic rice plants that express C4-type phospho enol pyruvate carboxykinase from Urochloa panicoides. Plant Physiology, 124(1), 163-172. https://doi.org/10.1104/pp.124.1.163
Suzuki, Y. A., Kelleher, S. L., Yalda, D., Wu, L., Huang, J., Huang, N., & Lonnerdal, B. (2003). Expression, characterization, and biologic activity of recombinant human lactoferrin in rice. Journal of Pediatric Gastroenterology and Nutrition, 36(2), 190-199. https://doi.org/10.1097/00005176-200302000-00007
Takaiwa, F., Ogawa, M., & Okita, T. W. (1999). Rice glutelins. In Seed Proteins (pp. 401-425). Springer.
Takeuchi, Y., Hayasaka, H., Chiba, B., Tanaka, I., Shimano, T., Yamagishi, M., Nagano, K., Sasaki, T., & Yano, M. (2001). Mapping quantitative trait loci controlling cool-temperature tolerance at booting stage in temperate japonica rice. Breeding Science, 51(3), 191-197. https://doi.org/10.1270/jsbbs.51.191
Toriyama, K., Arimoto, Y., Uchimiya, H., & Hinata, K. (1988). Transgenic rice plants after direct gene transfer into protoplasts. Bio/Technology, 6(9), 1072-1074. https://doi.org/10.1038/nbt0988-1072
Uchimiya, H., Fushimi, T., Hashimoto, H., Harada, H., Syono, K., & Sugawara, Y. (1986). Expression of a foreign gene in callus derived from DNA-treated protoplasts of rice (Oryza sativa L.). Molecular and General Genetics MGG, 204(2), 204-207. https://doi.org/10.1007/BF00425499
Wakita, Y., Otani, M., Iba, K., & Shimada, T. (1998). Co-integration, co-expression and co-segregation of an unlinked selectable marker gene and NtFAD3 gene in transgenic rice plants produced by particle bombardment. Genes & Genetic Systems, 73(4), 219-226. https://doi.org/10.1266/ggs.73.219
Washida, H., Wu, C. Y., Suzuki, A., Yamanouchi, U., Akihama, T., Harada, K., & Takaiwa, F. (1999). Identification of cis-regulatory elements required for endosperm expression of the rice storage protein glutelin gene *GluB-1*. Plant Molecular Biology, 40(1), 1-12. https://doi.org/10.1023/a:1026459229671
Yamada, Y., Yang, Z. Q., & Tang, D. T. (1986). Plant regeneration from protoplast-derived callus of rice (Oryza sativa L.). Plant Cell Reports, 5(2), 85-88. https://doi.org/10.1007/BF00269240
Yamaguchi, T., Kuroda, M., Yamakawa, H., Ashizawa, T., Hirayae, K., Kurimoto, L., Shinya, T., & Shibuya, N. (2009). Suppression of a phospholipase D gene, OsPLDbeta1, activates defense responses and increases disease resistance in rice. Plant Physiology, 150(1), 308-319. https://doi.org/10.1104/pp.108.131979
Zong-Xiu, S., Cheng-Zhang, Z., Kang-le, Z., Xiu-Fang, Q., & Ya-Ping, F. (1983). Somaclonal genetics of rice, Oryza sativa L. Theoretical and Applied Genetics, 67(1), 67-73. https://doi.org/10.1007/BF00303925
Asad, S., & Arshad, M. (2011). Silicon carbide whisker-mediated plant transformation. Properties and applications of silicon carbide, 345-358.
Chen, Z., Debernardi, J. M., Dubcovsky, J., & Gallavotti, A. (2022). Recent advances in crop transformation technologies. Nature Plants, 8(12), 1343-1351. https://doi.org/10.1038/s41477-022-01295-8
Fujimoto, H., Itoh, K., Yamamoto, M., Kyozuka, J., & Shimamoto, K. (1993). Insect resistant rice generated by introduction of a modified delta-endotoxin gene of Bacillus thuringiensis. Bio/Technology, 11(10), 1151-1155. https://doi.org/10.1038/nbt1093-1151
Fujimura, T., Sakurai, M., Akagi, H., Negishi, T., & Hirose, A. (1985). Regeneration of rice plants from protoplasts. Plant Tissue Culture Letters, 2(2), 74-75. https://doi.org/10.5511/plantbiotechnology1984.2.74
Hayashi, H., & Murata, N. (1998). Genetically engineered enhancement of salt tolerance in higher plants. In Stress Response of Photosynthetic Organisms: Molecular Mechanisms and Molecular Regulation (pp. 133-148). Elsevier.
Hiei, Y., & Komari, T. (2008). Agrobacterium-mediated transformation of rice using immature embryos or calli induced from mature seed. Nature Protocols, 3(5), 824-834. https://doi.org/10.1038/nprot.2008.46
Hiei, Y., Ohta, S., Komari, T., & Kumashiro, T. (1994). Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA. The Plant Journal, 6(2), 271-282. https://doi.org/10.1046/j.1365-313x.1994.6020271.x
Isshiki, M., Morino, K., Nakajima, M., Okagaki, R. J., Wessler, S. R., Izawa, T., & Shimamoto, K. (1998). A naturally occurring functional allele of the rice waxy locus has a GT to TT mutation at the 5' splice site of the first intron. The Plant Journal, 15(1), 133-138. https://doi.org/10.1046/j.1365-313x.1998.00189.x
Karasek, K. R., Bradley, S. A., Donner, J. T., Martin, M. R., Haynes, K. L., & Yeh, H. C. (1989). Composition and microstructure of silicon carbide whiskers. Journal of Materials Science, 24(5), 1617-1622. https://doi.org/10.1007/BF01105681
Katsube, T., Kurisaka, N., Ogawa, M., Maruyama, N., Ohtsuka, R., Utsumi, S., & Takaiwa, F. (1999). Accumulation of soybean glycinin and its assembly with the glutelins in rice. Plant Physiology, 120(4), 1063-1074. https://doi.org/10.1104/pp.120.4.1063
Kawahigashi, H., Hirose, S., Ohkawa, H., & Ohkawa, Y. (2003). Transgenic rice plants expressing human CYP1A1 exude herbicide metabolites from their roots. Plant Science, 165(2), 373-381. https://doi.org/10.1016/S0168-9452(03)00197-3
Komamine, A. (2003). My way with plant cell cultures: significance of experimental systems in plant biology. In Vitro Cellular & Developmental Biology - Plant, 39(2), 63. https://doi.org/10.1079/IVP2002404
Komari, T., Takakura, Y., Ueki, J., Kato, N., Ishida, Y., & Hiei, Y. (2006). Binary vectors and super-binary vectors. Methods in Molecular Biology, 343, 15-42. https://doi.org/10.1385/1-59745-130-4:15
Kyozuka, J., Hayashi, Y., & Shimamoto, K. (1987). High frequency plant regeneration from rice protoplasts by novel nurse culture methods. Molecular and General Genetics MGG, 206(3), 408-413. https://doi.org/10.1007/BF00428879
Li, D., Nanseki, T., Chomei, Y., & Kuang, J. (2023). A review of smart agriculture and production practices in Japanese large-scale rice farming. Journal of the Science of Food and Agriculture, 103(4), 1609-1620. https://doi.org/10.1002/jsfa.12204
Masuda, H., Kobayashi, T., Ishimaru, Y., Takahashi, M., Aung, M. S., Nakanishi, H., Mori, S., & Nishizawa, N. K. (2013). Iron-biofortification in rice by the introduction of three barley genes participated in mugineic acid biosynthesis with soybean ferritin gene. Frontiers in Plant Science, 4, 132. https://doi.org/10.3389/fpls.2013.00132
Matsumura, T., Tabayashi, N., Kamagata, Y., Souma, C., & Saruyama, H. (2002). Wheat catalase expressed in transgenic rice can improve tolerance against low temperature stress. Physiologia Plantarum, 116(3), 317-327. https://doi.org/10.1034/j.1399-3054.2002.1160306.x
Matsuoka, M., Furbank, R. T., Fukayama, H., & Miyao, M. (2001). Molecular Engineering of C4 Photosynthesis. Annual Review of Plant Physiology and Plant Molecular Biology, 52(1), 297-314. https://doi.org/10.1146/annurev.arplant.52.1.297
Matsushita, J., Otani, M., Wakita, Y., Tanaka, O., & Shimada, T. (1999). Transgenic plant regeneration through silicon carbide whisker-mediated transformation of rice (Oryza sativa L.). Breeding Science, 49(1), 21-26. https://doi.org/10.1270/jsbbs.49.21
Nakagahra, M., Okuno, K., & Vaughan, D. (1997). Rice genetic resources: history, conservation, investigative characterization and use in Japan. Plant Molecular Biology, 35(1-2), 69-77. https://doi.org/10.1023/A:1005784431759
Nakase, M., Aoki, N., Matsuda, T., & Adachi, T. (1997). Characterization of a novel rice bZIP protein which binds to the alpha-globulin promoter. Plant Molecular Biology, 33(3), 513-522. https://doi.org/10.1023/a:1005784717782
Niizeki, H., & Oono, K. (1968). Induction of haploid rice plant from anther culture. Proceedings of the Japan Academy, 44(6), 554-557. https://doi.org/10.2183/pjab1945.44.554
Ozawa, K. (2012). A high-efficiency Agrobacterium-mediated transformation system of rice (Oryza sativa L.). In Transgenic Plants: Methods and Protocols (pp. 51-57). Humana Press. https://doi.org/10.1007/978-1-61779-558-9_5
Ozawa, K., & Komamine, A. (1989). Establishment of a system of high-frequency embryogenesis from long-term cell suspension cultures of rice (Oryza sativa L.). Theoretical and Applied Genetics, 77(2), 205-211. https://doi.org/10.1007/BF00266188
Radi, S. H., & Maeda, E. (1987). Ultrastructures of rice scutellum cultured with attached root using two separate media as compared to the intact seedlings. Japanese Journal of Crop Science, 56(1), 73-84. https://doi.org/10.1626/jcs.56.73
Satoh, K., Kondoh, H., Sasaya, T., Shimizu, T., Choi, I. R., Omura, T., & Kikuchi, S. (2010). Selective modification of rice (Oryza sativa) gene expression by rice stripe virus infection. Journal of General Virology, 91(Pt 1), 294-305. https://doi.org/10.1099/vir.0.015990-0
Sharoni, A. M., Nuruzzaman, M., Satoh, K., Shimizu, T., Kondoh, H., Sasaya, T., Choi, I. R., Omura, T., & Kikuchi, S. (2011). Gene structures, classification and expression models of the AP2/EREBP transcription factor family in rice. Plant and Cell Physiology, 52(2), 344-360. https://doi.org/10.1093/pcp/pcq196
Shimamoto, K., Terada, R., Izawa, T., & Fujimoto, H. (1989). Fertile transgenic rice plants regenerated from transformed protoplasts. Nature, 338(6212), 274-276. https://doi.org/10.1038/338274a0
Sukegawa, S., Nureki, O., Toki, S., & Saika, H. (2023). Genome editing in rice mediated by miniature size Cas nuclease SpCas12f. Frontiers in Genome Editing, 5, 1138843. https://doi.org/10.3389/fgeed.2023.1138843
Sun, Z., Sun, L., & Shu, L. (1991). Utilization of somaclonal variation in rice breeding. In Rice Biotechnology (pp. 328-346). CAB International.
Suzuki, S., Murai, N., Burnell, J. N., & Arai, M. (2000). Changes in photosynthetic carbon flow in transgenic rice plants that express C4-type phospho enol pyruvate carboxykinase from Urochloa panicoides. Plant Physiology, 124(1), 163-172. https://doi.org/10.1104/pp.124.1.163
Suzuki, Y. A., Kelleher, S. L., Yalda, D., Wu, L., Huang, J., Huang, N., & Lonnerdal, B. (2003). Expression, characterization, and biologic activity of recombinant human lactoferrin in rice. Journal of Pediatric Gastroenterology and Nutrition, 36(2), 190-199. https://doi.org/10.1097/00005176-200302000-00007
Takaiwa, F., Ogawa, M., & Okita, T. W. (1999). Rice glutelins. In Seed Proteins (pp. 401-425). Springer.
Takeuchi, Y., Hayasaka, H., Chiba, B., Tanaka, I., Shimano, T., Yamagishi, M., Nagano, K., Sasaki, T., & Yano, M. (2001). Mapping quantitative trait loci controlling cool-temperature tolerance at booting stage in temperate japonica rice. Breeding Science, 51(3), 191-197. https://doi.org/10.1270/jsbbs.51.191
Toriyama, K., Arimoto, Y., Uchimiya, H., & Hinata, K. (1988). Transgenic rice plants after direct gene transfer into protoplasts. Bio/Technology, 6(9), 1072-1074. https://doi.org/10.1038/nbt0988-1072
Uchimiya, H., Fushimi, T., Hashimoto, H., Harada, H., Syono, K., & Sugawara, Y. (1986). Expression of a foreign gene in callus derived from DNA-treated protoplasts of rice (Oryza sativa L.). Molecular and General Genetics MGG, 204(2), 204-207. https://doi.org/10.1007/BF00425499
Wakita, Y., Otani, M., Iba, K., & Shimada, T. (1998). Co-integration, co-expression and co-segregation of an unlinked selectable marker gene and NtFAD3 gene in transgenic rice plants produced by particle bombardment. Genes & Genetic Systems, 73(4), 219-226. https://doi.org/10.1266/ggs.73.219
Washida, H., Wu, C. Y., Suzuki, A., Yamanouchi, U., Akihama, T., Harada, K., & Takaiwa, F. (1999). Identification of cis-regulatory elements required for endosperm expression of the rice storage protein glutelin gene *GluB-1*. Plant Molecular Biology, 40(1), 1-12. https://doi.org/10.1023/a:1026459229671
Yamada, Y., Yang, Z. Q., & Tang, D. T. (1986). Plant regeneration from protoplast-derived callus of rice (Oryza sativa L.). Plant Cell Reports, 5(2), 85-88. https://doi.org/10.1007/BF00269240
Yamaguchi, T., Kuroda, M., Yamakawa, H., Ashizawa, T., Hirayae, K., Kurimoto, L., Shinya, T., & Shibuya, N. (2009). Suppression of a phospholipase D gene, OsPLDbeta1, activates defense responses and increases disease resistance in rice. Plant Physiology, 150(1), 308-319. https://doi.org/10.1104/pp.108.131979
Zong-Xiu, S., Cheng-Zhang, Z., Kang-le, Z., Xiu-Fang, Q., & Ya-Ping, F. (1983). Somaclonal genetics of rice, Oryza sativa L. Theoretical and Applied Genetics, 67(1), 67-73. https://doi.org/10.1007/BF00303925
Published
2026-06-28
How to Cite
Lai, T., Li, C., He, B., Wang, X., Lang, H., Wang, S., & Wang, S. (2026). Contributions and application advances of japan in the development of rice genetic transformation technology systems. Revista De La Facultad De Agronomía De La Universidad Del Zulia, 43(3), e264336. Retrieved from https://www.produccioncientificaluz.org/index.php/agronomia/article/view/45775
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Copyright (c) 2026 Taiye Lai, Chao Li, Bing He, Xiaohang Wang, Hong Lang, Shuai Wang
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