Effects of Zinc supplementation in Magnesium alloys on hemogram and erythrocyte osmotic fragility
Abstract
Magnesium and zinc are important trace elements that have important roles in metabolism, immune function and blood cell formation. The effects of dietary supplementation with magnesium and zinc on erythrocyte, leukocyte, platelet parameters and erythrocyte osmotic fragility were studied here. Rats were implanted with several magnesium - zinc alloys in the muscle next to the femur. The animals were divided into seven groups and in the 4th and 7th weeks blood samples were collected for hemogram analysis and osmotic fragility test. The groups were: Control, magnesium (4th and 7th week), magnesium + zinc1 (4th and 7th week) and magnesium + zinc3 (4th and 7th week). The mean corpuscular volume and red blood cell distribution frequency values were significantly higher in the magnesium + zinc3 (4th week) group, while the mean corpuscular volume value was lower in the magnesium (7th week) group. A decrease in lymphocyte count and percentage was observed only in the magnesium + zinc1 (4th week) group, whereas the increase was found in the magnesium (7th week) group. Neutrophil count and percentage were significantly increased in magnesium + zinc1 (4th week) whereas decreased in both groups of magnesium + zinc at 7th week. Hemolysis levels at sodium chloride 0.4 % and 0.5 % concentration were no less than 94 and 86 %, respectively, for the magnesium + zinc3 (7th week) group. It seems that the magnesium + zinc mixture may influence erythropoiesis and might produce macrocytosis as well as anisocytosis changes, which could normalize over time by physiological adaptation. The stable increments achieved in the magnesium group also indicate a more constant role for magnesium in homeostasis of blood elements.
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