Molecular study of the “Escoba Blanca” variety of Sesamum indicum L. used in Paraguay
Keywords:
genetic diversity, genotype, microsatellite markers, Sesamum indicum L.
Abstract
The high quality of sesame seeds originating in the country has led Paraguay to be among the main exporters. Of the varieties available in Paraguayan territory, the most widespread is ‘‘Escoba Blanca’’, which, possibly due to the multiplication process, could promote changes in its allele frequency, diversity, and genetic purity. This work was carried out, aiming to determine the genetic differentiation between 50 populations/seedbeds/banks from seven Paraguayan companies collecting ‘‘Escoba Blanca’’ sesame, using microsatellite markers. These seven banks/companies/cooperatives collect and represent samples from all the producers/seedbeds located in different departments of the Eastern and Western Region (Chaco) of the country, with whom they work, market, and collect sesame. Plant tissue was obtained to extract DNA, from seedlings planted especially for the purpose, using all the included samples/accessions. Six microsatellite markers were used: GBssrsa184, GBssrsa123, GBssrsa182, GBssrsa108, GBssrsa08, and GBssrsa72. The following were calculated: number and frequency of alleles, distance/groupings, differentiation between populations, and their genetic structure. The mean number of alleles per locus ranged from 1.33 to 3.00. In the markers, GBssrsa184 and GBssrsa108, three populations presented a higher frequency of alleles. The populations examined exhibited a wide degree of genetic differentiation between them, with the identification of four groups, with greater and less purity respectively.
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References
Abdellatef, E., Sirelkhatem, R., Mohamed, A. M. M., Radwan, K. H., & Khalafalla, M. M. (2008). Study of genetic diversity in sudanese sesame (Sesamum indicum L.) germplasm using random amplified polymorphic DNA (RAPD) markers. African Journal of Biotechnology, 7(24), 4423–4427. https://www.ajol.info/index.php/ajb/article/view/59603
Alda, J., Marcos-Merino, J., & Gallego, R. (2019). Extracción de ADN con material cotidiano: desarrollo de una estrategia interdisciplinar a partir de sus fundamentos científicos. Educación Química, 30(1), 58-69. https://doi.org/10.22201/fq.18708404e.2019.1.65732
Ali, G. M., Yasumoto, S., & Seki-Katsuta, M. (2007). Assessment of genetic diversity in sesame (Sesamum indicum L.) detected by amplified fragment length polymorphism markers. Electronic Journal of Biotechnology, 10(1), 12-23. https://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000100002&lng=es&nrm=iso
Angulo, L., Perichi, G. A., Castro, L. D., & Figueroa, R. (2020). Comparación de diferentes métodos de extracción de ADN genómico en babosas plagas (mollusca: gasterópoda). Revista Científica ECOCIENCIA, 7(4), 35-49. https://doi.org/10.21855/ecociencia.74.380
Arriel, N. H. C., Di Mauro, A., Di Mauro S., Bakke, O. A., Unêda-Trevisoli, S., Costa, M., Capeloto, A., & Corrado, A. R. (2006). Técnicas multivariadas na determinação da diversidade genética em gergelim usando marcadores RAPD. Pesquisa Agropecuária Brasileira, 41(5), 801-809. https://doi.org/10.1590/S0100-204X2006000500012
Cho, Y.I., Park, J.H., Lee, C.W., Ra, W.H., Chung, J.W., Lee, J.R., Ma, K.H., Lee, S.Y., Lee, K.S., Lee, M.C., & Park, Y.J. (2011). Evaluation of the genetic diversity and population structure of sesame (Sesamum indicum L.) using microsatellite markers. Genes & Genomics, 33(2), 187–195. https://doi.org/10.1007/s13258-010-0130-6
Dar, A. A., Mudigunda, S, Mittal, P. K., & Arumugam, N. (2017) Comparative assessment of genetic diversity in Sesamum indicum L. using RAPD and SSR markers. 3 Biotech, 7(10), 1-12. https://doi.org/10.1007/s13205-016-0578-4
Dossa, K., Wei, X., Zhang, Y., Fonceka, D., Yang, W., Diouf, D., Liao, B., Cissé, N., & Zhang, X. (2016). Analysis of genetic diversity and population structure of sesame accessions from Africa and Asia as major centers of its cultivation. Genes, 7(4), 1-13. https://doi.org/10.3390/genes7040014
Gediya, L., Patel, D., Kumar, S., Kumar, P., Parmar, D., & Patel, S. (2019). Phenotypic variability, path analysis and molecular diversity analysis in chickpea (Cicer arietinum L.). Vegetos, 32(2), 167-180. https://doi.org/10.1007/s42535-019-00020-9
González, A., & Causarano, H. (2014). Destino del nitrógeno aplicado en un cultivo de sésamo (Sesamum indicum L.) en un suelo degradado de Paraguay. Acta Agronómica, 63(3), 253-261. https://doi.org/10.15446/acag.v63n3.42259
Laurentin, H., & Karlovsky, P. (2006). Genetic relationship and diversity in a sesame (Sesamum indicum L.) germplasm collection using amplified fragment length polymorphism (AFLP). BMC Genetics, 7(1), 1–10. https://doi.org/10.1186/1471-2156-7-10
Martínez-López, O. R. (2017). El Pampa Chaqueño: raza bovina local del Paraguay. Argumentos técnicos y científicos que sustentan su reconocimiento como raza local. Artes Gráficas Visual. https://isbn.bibliotecanacional.gov.py/catalogo.php?mode=detalle&nt=11326
Martínez-López, O. R., Barbosa, S., Martínez, A. M, Delgado, J. V., & Landi, V. (2019a). Distancias genéticas entre dos poblaciones de bovinos criollos paraguayos. AICA, 14,126-136. https://www.aicarevista.com/n%C3%BAmeros/vol%C3%BAmen-14-2019/
Martínez-López, O. R., Barbosa, S., Martínez, A. M., Delgado, J. V., & Landi, V. (2019b). Perfil genético de dos poblaciones bovinas criollas de Paraguay. AICA, 14, 141-153. https://www.aicarevista.com/n%C3%BAmeros/vol%C3%BAmen-14-2019/
Melgarejo, M., Galeano, A., Amarilla, D., Maidana, E., Bogado, M., Franco, R., Mendoza, M., Colman, P., Silvero, O., Lugo, W., & Da Silva, M. (2020). Efecto de diferentes densidades de siembra sobre las características agronómicas del ajonjolí (Sesamum indicum L.) en el distrito de Curuguaty. Idesia (Arica), 38(3), 107-112. https://dx.doi.org/10.4067/S0718-34292020000300107
Nei, M., & Takezaki, N. (1996). The root of the phylogenetic tree of human populations. Molecular Biology and Evolution, 13(1), 170-177. https://doi.org/10.1093/oxfordjournals.molbev.a025553
Nyongesa, B., Were, B., Gudu, S., Dangasuk, O., & Onkware, A. (2013). Genetic diversity in cultivated sesame (Sesamum indicum L.) and related wild species in East Africa. Journal of Crop Science and Biotechnology, 16(1), 9-15. https://doi.org/10.1007/s12892-012-0114-y
Pandey, S., Das, A., Rai, P., & Dasgupta, T. (2015). Morphological and genetic diversity assessment of sesame (Sesamum indicum L.) accessions differing in origin. Physiology and Molecular Biology of Plants, 21(4), 519-529. https://doi.org/10.1007/s12298-015-0322-2
Pham, T. (2011). Analyses of Genetic Diversity and Desirable Traits in Sesame (Sesamum indicum L., Pedaliaceae): Implication for Breeding and Conservation [Doctoral Thesis, Swedish University of Agricultural Sciences]. https://res.slu.se/id/publ/33897
Rabery, S., Enciso, V., Franco, R., & Lezcano, J. (2020). Incidencia del riego complementario en el rendimiento de granos de sésamo (Sesamun indicum L.). Investigación Agraria, 22(2), 75-81. https://doi.org/10.18004/investig.agrar.2020.diciembre.2202642
Suazo, T., Miranda, S., Rivers, E., Lacayo, M., & Tenorio, D. (2020). Evaluación de metodologías de extracción de ADN de plantas recalcitrantes. Revista Torreón Universitario, 9(24), 45-57. https://doi.org/10.5377/torreon.v9i24.9723
Surapaneni, M., Yepuri, V., Vemireddy, R., Ghanta, A., & Siddiq, E. (2014). Development and characterization of microsatellite markers in Indian sesame (Sesamum indicum L.). Molecular Breeding, 34(3), 1185-1200. https://doi.org/10.1007/s11032-014-0109-0
Teklu, D., Shimelis, H., Tesfaye, A., Mashilo, J., Zhang, X., Zhang, Y., Dossa, K., & Shayanowako, A. (2021). Genetic variability and population structure of Ethiopian sesame (Sesamum indicum L.) germplasm assessed through phenotypic traits and simple sequence repeats markers. Plants, 10(6), 1129. https://doi.org/10.3390/plants10061129
Teklu, D., Shimelis, H., Tesfaye, A., & Shayanowako, A. (2022). Analyses of genetic diversity and population structure of sesame (Sesamum indicum L.) germplasm collections through seed oil and fatty acid compositions and SSR markers. Journal of Food Composition and Analysis, 110(104545),1-12. https://doi.org/10.1016/j.jfca.2022.104545
Zhang, J., Yang, J., Lv, Y., Zhang, X., Xia, C., Zhao, H., & Wen, C. (2023). Genetic diversity analysis and variety identification using SSR and SNP markers in melon. BMC Plant Biology, 23(39), 2-10. https://doi.org/10.1186/s12870-023-04056-7
Alda, J., Marcos-Merino, J., & Gallego, R. (2019). Extracción de ADN con material cotidiano: desarrollo de una estrategia interdisciplinar a partir de sus fundamentos científicos. Educación Química, 30(1), 58-69. https://doi.org/10.22201/fq.18708404e.2019.1.65732
Ali, G. M., Yasumoto, S., & Seki-Katsuta, M. (2007). Assessment of genetic diversity in sesame (Sesamum indicum L.) detected by amplified fragment length polymorphism markers. Electronic Journal of Biotechnology, 10(1), 12-23. https://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000100002&lng=es&nrm=iso
Angulo, L., Perichi, G. A., Castro, L. D., & Figueroa, R. (2020). Comparación de diferentes métodos de extracción de ADN genómico en babosas plagas (mollusca: gasterópoda). Revista Científica ECOCIENCIA, 7(4), 35-49. https://doi.org/10.21855/ecociencia.74.380
Arriel, N. H. C., Di Mauro, A., Di Mauro S., Bakke, O. A., Unêda-Trevisoli, S., Costa, M., Capeloto, A., & Corrado, A. R. (2006). Técnicas multivariadas na determinação da diversidade genética em gergelim usando marcadores RAPD. Pesquisa Agropecuária Brasileira, 41(5), 801-809. https://doi.org/10.1590/S0100-204X2006000500012
Cho, Y.I., Park, J.H., Lee, C.W., Ra, W.H., Chung, J.W., Lee, J.R., Ma, K.H., Lee, S.Y., Lee, K.S., Lee, M.C., & Park, Y.J. (2011). Evaluation of the genetic diversity and population structure of sesame (Sesamum indicum L.) using microsatellite markers. Genes & Genomics, 33(2), 187–195. https://doi.org/10.1007/s13258-010-0130-6
Dar, A. A., Mudigunda, S, Mittal, P. K., & Arumugam, N. (2017) Comparative assessment of genetic diversity in Sesamum indicum L. using RAPD and SSR markers. 3 Biotech, 7(10), 1-12. https://doi.org/10.1007/s13205-016-0578-4
Dossa, K., Wei, X., Zhang, Y., Fonceka, D., Yang, W., Diouf, D., Liao, B., Cissé, N., & Zhang, X. (2016). Analysis of genetic diversity and population structure of sesame accessions from Africa and Asia as major centers of its cultivation. Genes, 7(4), 1-13. https://doi.org/10.3390/genes7040014
Gediya, L., Patel, D., Kumar, S., Kumar, P., Parmar, D., & Patel, S. (2019). Phenotypic variability, path analysis and molecular diversity analysis in chickpea (Cicer arietinum L.). Vegetos, 32(2), 167-180. https://doi.org/10.1007/s42535-019-00020-9
González, A., & Causarano, H. (2014). Destino del nitrógeno aplicado en un cultivo de sésamo (Sesamum indicum L.) en un suelo degradado de Paraguay. Acta Agronómica, 63(3), 253-261. https://doi.org/10.15446/acag.v63n3.42259
Laurentin, H., & Karlovsky, P. (2006). Genetic relationship and diversity in a sesame (Sesamum indicum L.) germplasm collection using amplified fragment length polymorphism (AFLP). BMC Genetics, 7(1), 1–10. https://doi.org/10.1186/1471-2156-7-10
Martínez-López, O. R. (2017). El Pampa Chaqueño: raza bovina local del Paraguay. Argumentos técnicos y científicos que sustentan su reconocimiento como raza local. Artes Gráficas Visual. https://isbn.bibliotecanacional.gov.py/catalogo.php?mode=detalle&nt=11326
Martínez-López, O. R., Barbosa, S., Martínez, A. M, Delgado, J. V., & Landi, V. (2019a). Distancias genéticas entre dos poblaciones de bovinos criollos paraguayos. AICA, 14,126-136. https://www.aicarevista.com/n%C3%BAmeros/vol%C3%BAmen-14-2019/
Martínez-López, O. R., Barbosa, S., Martínez, A. M., Delgado, J. V., & Landi, V. (2019b). Perfil genético de dos poblaciones bovinas criollas de Paraguay. AICA, 14, 141-153. https://www.aicarevista.com/n%C3%BAmeros/vol%C3%BAmen-14-2019/
Melgarejo, M., Galeano, A., Amarilla, D., Maidana, E., Bogado, M., Franco, R., Mendoza, M., Colman, P., Silvero, O., Lugo, W., & Da Silva, M. (2020). Efecto de diferentes densidades de siembra sobre las características agronómicas del ajonjolí (Sesamum indicum L.) en el distrito de Curuguaty. Idesia (Arica), 38(3), 107-112. https://dx.doi.org/10.4067/S0718-34292020000300107
Nei, M., & Takezaki, N. (1996). The root of the phylogenetic tree of human populations. Molecular Biology and Evolution, 13(1), 170-177. https://doi.org/10.1093/oxfordjournals.molbev.a025553
Nyongesa, B., Were, B., Gudu, S., Dangasuk, O., & Onkware, A. (2013). Genetic diversity in cultivated sesame (Sesamum indicum L.) and related wild species in East Africa. Journal of Crop Science and Biotechnology, 16(1), 9-15. https://doi.org/10.1007/s12892-012-0114-y
Pandey, S., Das, A., Rai, P., & Dasgupta, T. (2015). Morphological and genetic diversity assessment of sesame (Sesamum indicum L.) accessions differing in origin. Physiology and Molecular Biology of Plants, 21(4), 519-529. https://doi.org/10.1007/s12298-015-0322-2
Pham, T. (2011). Analyses of Genetic Diversity and Desirable Traits in Sesame (Sesamum indicum L., Pedaliaceae): Implication for Breeding and Conservation [Doctoral Thesis, Swedish University of Agricultural Sciences]. https://res.slu.se/id/publ/33897
Rabery, S., Enciso, V., Franco, R., & Lezcano, J. (2020). Incidencia del riego complementario en el rendimiento de granos de sésamo (Sesamun indicum L.). Investigación Agraria, 22(2), 75-81. https://doi.org/10.18004/investig.agrar.2020.diciembre.2202642
Suazo, T., Miranda, S., Rivers, E., Lacayo, M., & Tenorio, D. (2020). Evaluación de metodologías de extracción de ADN de plantas recalcitrantes. Revista Torreón Universitario, 9(24), 45-57. https://doi.org/10.5377/torreon.v9i24.9723
Surapaneni, M., Yepuri, V., Vemireddy, R., Ghanta, A., & Siddiq, E. (2014). Development and characterization of microsatellite markers in Indian sesame (Sesamum indicum L.). Molecular Breeding, 34(3), 1185-1200. https://doi.org/10.1007/s11032-014-0109-0
Teklu, D., Shimelis, H., Tesfaye, A., Mashilo, J., Zhang, X., Zhang, Y., Dossa, K., & Shayanowako, A. (2021). Genetic variability and population structure of Ethiopian sesame (Sesamum indicum L.) germplasm assessed through phenotypic traits and simple sequence repeats markers. Plants, 10(6), 1129. https://doi.org/10.3390/plants10061129
Teklu, D., Shimelis, H., Tesfaye, A., & Shayanowako, A. (2022). Analyses of genetic diversity and population structure of sesame (Sesamum indicum L.) germplasm collections through seed oil and fatty acid compositions and SSR markers. Journal of Food Composition and Analysis, 110(104545),1-12. https://doi.org/10.1016/j.jfca.2022.104545
Zhang, J., Yang, J., Lv, Y., Zhang, X., Xia, C., Zhao, H., & Wen, C. (2023). Genetic diversity analysis and variety identification using SSR and SNP markers in melon. BMC Plant Biology, 23(39), 2-10. https://doi.org/10.1186/s12870-023-04056-7
Published
2024-02-09
How to Cite
Martínez-López, R., Esfrain Pereira, W., Arrua, A., Fernández , D., & Centurión, L. (2024). Molecular study of the “Escoba Blanca” variety of Sesamum indicum L. used in Paraguay. Revista De La Facultad De Agronomía De La Universidad Del Zulia, 41(1), e244104. Retrieved from https://www.produccioncientificaluz.org/index.php/agronomia/article/view/41585
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Crop Production
Copyright (c) 2024 Roberto Martínez-López, Walter Esfrain Pereira, Andrea Alejandra Arrua, Danilo Fernández Ríos, Liz Mariela Centurión
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