Study of synergistic effect of combined application of tebuconazole with two biocontrol agents for management of Fusarium crown rot in durum wheat
Keywords:
Bacillus, combined control, fungicide, Fusarium culmorum, wheat seedlings
Abstract
The in vitro and growth chamber, tests were conducted in order to assess the effects of B. amyloliquefaciens B18 and B. subtilis S8 strains each alone and in combination with tebuconazole against F. culmorum (FC) isolate responsible of Fusarium crown rot (FCR) in durum wheat. The in vitro growth of B18 and S8 strains was unaffected by 30 µg.mL-1 tebuconazole. The Bacillus strains (at 106 CFU.mL-1) and tebuconazole, each alone, reduced the mycelial growth, this effect was significantly improved when they were combined (inhibition of more than 92 %). In growth chamber experiments, efficacy against FCR was significantly higher when integrating Bacillus strains and tebuconazole than by either alone; control efficacy of tebuconazole at 30 µg.mL-1 in combination with S8 and B18 strains reached 90.91 and 95.45 %, respectively. The obtained results indicated that combination of tebuconazole with the biocontrol agents B18 and S8 synergistically improved control efficiency of the fungicide against FCR of wheat.
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References
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Huang, H., Zhao, Y., Fan, L., Jin, Q., Yang, G., & Xu Z. (2020). Improvement o manganese phytoremediation by Broussonetia papyrifera with two plant growth promoting (PGP) Bacillus species. Chemosphere, 127614. https://doi.org/10.1016/j.chemosphere.2020.127614
Ji, X., Li, J., Meng, Z., Zhang, S., Dong, B., & Qiao K. (2019). Synergistic effect of combined application of a new fungicide fluopimomide with a biocontrol agent Bacillus methylotrophicus TA-1 for management of gray mold in Tomato. Plant Diseases, 103(8), 1991-1997. https://doi.org/10.1094/PDIS-01-19-0143-RE
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Khedher, S. B., Mejdoub-Trabelsi, B., & Tounsi S. (2020). Biological potential of Bacillus subtilis V26 for the control of Fusarium wilt and tuber dry rot on potato caused by Fusarium species and the promotion of plant growth. Biological Control, 104, 104444. https://doi.org/10.1016/j.biocontrol.2020.104444
Lee, T., Park, D., Kim, K., Lim, S. M., Yu, N.H., Kim, S., & Kim J. C. (2017). Characterization of Bacillus amyloliquefaciens DA12 showing potent antifungal activity against mycotoxigenic Fusarium species. The Plant Pathology Journal, 33(5), 499-507. https://doi.org/10.5423/PPJ.FT.06.2017.0126
Lugtenberg, B., and& Kamilova, F. (2009). Plant‐growth‐promoting rhizo‐bacteria. Annual Review of Microbiology, 63, 541-556. https://doi.org/10.1146/ annurev.micro.62.081307.162918
McKinney, H.H. (1923). Influence of soil temperature and moisture on infection of wheat seedlings by Helminthosporium sativum. Journal of Agricultural Research, 26, 195-217.
Odds, F. C., Brown, A. J. P., & Gow N. A. R. (2003). Antifungal agents: mechanisms of action. Trends in Microbiology, 11, 272-279. https://doi.org/10.1016/S0966-842X(03)00117-3
Palmieri, D., Laniri, G., Del Grosso, C., Barone, G., De Curtis, F., Castoria, R., & Lima G. (2022). Advances and perspectives in the use of biocontrol agents against fungal plant diseases. Horticulturae, 8, 577. https://doi.org/10.3390/horticulturae8070577
Rotolo, C., De Miccolis Angelini, R. M., Dongiovanni, C., Pollastro, S., Fumarola, G., Di Carolo, M., Perrelli, D., Natale, P., & Faretra F. (2018). Use of biocontrol agents and botanicals in integrated management of Botrytis cinerea in table-grape vineyards. Journal of Agriculture and Horticulture Research, 74, 715-725. https://doi.org/10.1002/ps.4767
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Vincent J. M. (1947). Distortion of fungal hyphae in the presence of certain inhibitors. Nature, 159:850. https://doi.org/10.1038/159850b0
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Yin, Y. N., Liu, X., Li, B., & Ma Z. H. (2009). Characterization of sterol demethylation inhibitor-resistant isolates of Fusarium asiaticum and F.graminearum collected from wheat in China. Phytopathology, 99, 487-497 https://doi.org/10.1094/PHYTO-99-5-0487
Yu, Y., Jiang, C., Wang, C., Chen, L., Li, H., Xu, Q., & Guo J. (2017). An improved strategy for stable biocontrol agents selecting to control rice sheath blight caused by Rhizoctonia solani. Microbiological Research, 203, 1-9. https://doi.org/10.1016/j.micres.2017.05.006
Zhang, L. G., Jia, X. J., Chen, C. J., & Zhou M. G. (2013). College of characterization of carbendazim sensitivity and trichothecene chemotypes of Fusarium graminearum in Jiangsu Province of China. Physiological and Molecular Plant Pathology, 84, 53–60 https://doi.org/10.1016/j.pmpp.2013.07.005
Zhao, P., Quan, C., Wang, Y., Wang, J., & Fan S. (2013). Bacillus amyloliquefaciens Q-426 as a potential biocontrol agent against Fusarium oxysporum f. sp. spinaciae. Journal of Basic Microbiology, 54(5), 448-456. https://doi.org/10.1002/jobm.201200414
Zhu, J., Tan, T., Shen, A., Yang, X., Yu, Y., Gao, C., & Zeng L. (2020). Biocontrol potential of Bacillus subtilis IBFCBF-4 against Fusarium wilt of watermelon. Journal of Plant Pathology, 102, 433–441. https://doi.org/10.1007/s42161-019-00457-6
Bellout, A., Bryant, Ch., & Abdellah G. (2020). The role of delivery of the use of agricultural techniques and extension services in increasing the capacity of wheat production to achieve food security in Algeria. Journal of Agriculture and Horticulture Research, 3(1), 1-9. https://www.opastpublishers.com/open-access-articles/the-role-of-delivery-of-the-use-of-agricultural-techniques-and-extension-services-in-increasing-the-capacity-of-wheat-pr.pdf
Bencheikh, A., Meziti, H., Daichi, B.M., Gharzouli, A., Belkadi, K., & Rouag N. (2022). Efficiency of durum wheat seeds biopriming by rhizobacteria in the biocontrol of Fusarium culmorum and Fusarium chlamydosporum infecting durum wheat in Algeria. Archives of Phytopathology and Plant Protection, 55(6), 653-675. https://doi.org/10.1080/03235408.2021.2025006
Bouanaka, H., Bellil, I., & Khelifi D. (2021). Multiple methods for varietal resistance assessment of durum wheat cultivars against Fusarium culmorum the causal agent of Fusarium head blight and crown rot in Algeria. Physiological and Molecular Plant Pathology, 115, 101683. https://doi.org/10.1016/j.pmpp.2021.101683
Brzezinska, M. S., Kalwasińska, A., Świątczak, J., Żero, K., & Jankiewicz U. (2020). Exploring the properties of chitinolytic Bacillus isolates for the pathogens biological control. Microbial Pathogenesis, 104462. https://doi.org/10.1016/j.micpath.2020.104462
Ceiro-Catasú, W. G., Rueda-Puente, E. O., Rondón-Fonseca, R., Sosa-Sánchez, O., & Holguín-Peña, R. J. (2022). Growth inhibition of Fusarium oxysporum f. sp. nicotianae by two in vitro poisoning methods with fungicides of different toxicological groups. Revista de la Facultad de Agronomía de la Universidad del Zulia, 39(3), e223944. https://doi.org/10.47280/RevFacAgron(LUZ).v39.n3.10
Chekali, S., Gargouri, S., Berraies, S., Nicol, M. J., & Nasraoui B. (2013). Impact of Fusarium foot and root rot on yield of cereals in Tunisia. Tunisian Journal of Plant Protection, 8(2), 75–86. http://www.iresa.agrinet.tn/tjpp/SiteWeb/PreviousIssues/TJPP8-2/2Chekali.pdf
Colby S. R. (1967). Calculating synergistic and antagonistic responses of herbicide combinations. Weeds, 15(1), 20-22. https://doi.org/10.2307/4041058
De Chaves, M.A., Reginatto, P., da Costa, B.S., de Paschoal, R.I., Teixeira, M.L., & Fuentefria A.M. (2022). Fungicide Resistance in Fusarium graminearum Species Complex. Current Microbiology, 79(62). http://doi.org/10.1007/s00284-021-02759-4
Desmyttere, H., Deweer, C., Muchembled, J., Sahmer, K., Jacquin, J., Coutte, F., & Jacques P. (2019). Antifungal activities of Bacillus subtilis lipopeptides to two Venturia inaequalis strains possessing different tebuconazole sensitivity. Frontiers in Microbiology, 10, 2327. https://doi.org/10.3389/fmicb.2019.02327
Fira, D., Dimkić, I., Berić, T., Lozo, J., & Stanković S. (2018). Biological control of plant pathogens by Bacillus species. Journal of Biotechnology, 285, 44-55. https://doi.org/10.1016/j.jbiotec.2018.07.044
Food and Agriculture Organization. (2018). Crop Prospects and Food Situation - Quarterly Global Report n°. 4, December. Rome. http://www.fao.org/faostat/en/#data/QC
Food and Agriculture Organization. (2019). Crop Prospects and Food Situation - Quarterly Global Report n°. 4, December. Rome. http://www.fao.org/faostat/en/#data/QC
Grey, W. E., and Mathre D. E. (1984). Reaction of spring barleys to common root rot and its effect on yield components. Canadian Journal of Plant Science, 64, 245-253. https://cdnsciencepub.com/doi/abs/10.4141/cjps84-038
Hellin, P., King, R., Urban, M., Hammond-Kosack, K.E., & Legrève A. (2018). The adaptation of Fusarium culmorum to DMI fungicides Is mediated by major transcriptome modifications in response to azole fungicide, including the overexpression of a PDR transporter (FcABC1). Frontiers in Microbiology, 9, 1385. https://doi.org/10.3389/fmicb.2018.01385
Hollaway, G. J., Evans, M. L., Wallwork, H., Dyson, C. B., & Mc Kay A. C. (2013). Yield loss in cereals, caused by Fusarium culmorum and F. pseudograminearum, is related to fungal DNA in soil prior to planting, rainfall, and cereal type. Plant Disease, 97, 977-982. https://doi.org/10.1094/PDIS-09-12-0867-RE
Huang, H., Zhao, Y., Fan, L., Jin, Q., Yang, G., & Xu Z. (2020). Improvement o manganese phytoremediation by Broussonetia papyrifera with two plant growth promoting (PGP) Bacillus species. Chemosphere, 127614. https://doi.org/10.1016/j.chemosphere.2020.127614
Ji, X., Li, J., Meng, Z., Zhang, S., Dong, B., & Qiao K. (2019). Synergistic effect of combined application of a new fungicide fluopimomide with a biocontrol agent Bacillus methylotrophicus TA-1 for management of gray mold in Tomato. Plant Diseases, 103(8), 1991-1997. https://doi.org/10.1094/PDIS-01-19-0143-RE
Kalam, S., Basu, A., & Podile A. R. (2020). Functional and molecular characterization of plant growth promoting Bacillus isolates from tomato rhizosphere. Heliyon, 6(8), e04734. https://doi.org/10.1016/j.heliyon.2020.e04734
Khedher, S. B., Mejdoub-Trabelsi, B., & Tounsi S. (2020). Biological potential of Bacillus subtilis V26 for the control of Fusarium wilt and tuber dry rot on potato caused by Fusarium species and the promotion of plant growth. Biological Control, 104, 104444. https://doi.org/10.1016/j.biocontrol.2020.104444
Lee, T., Park, D., Kim, K., Lim, S. M., Yu, N.H., Kim, S., & Kim J. C. (2017). Characterization of Bacillus amyloliquefaciens DA12 showing potent antifungal activity against mycotoxigenic Fusarium species. The Plant Pathology Journal, 33(5), 499-507. https://doi.org/10.5423/PPJ.FT.06.2017.0126
Lugtenberg, B., and& Kamilova, F. (2009). Plant‐growth‐promoting rhizo‐bacteria. Annual Review of Microbiology, 63, 541-556. https://doi.org/10.1146/ annurev.micro.62.081307.162918
McKinney, H.H. (1923). Influence of soil temperature and moisture on infection of wheat seedlings by Helminthosporium sativum. Journal of Agricultural Research, 26, 195-217.
Odds, F. C., Brown, A. J. P., & Gow N. A. R. (2003). Antifungal agents: mechanisms of action. Trends in Microbiology, 11, 272-279. https://doi.org/10.1016/S0966-842X(03)00117-3
Palmieri, D., Laniri, G., Del Grosso, C., Barone, G., De Curtis, F., Castoria, R., & Lima G. (2022). Advances and perspectives in the use of biocontrol agents against fungal plant diseases. Horticulturae, 8, 577. https://doi.org/10.3390/horticulturae8070577
Rotolo, C., De Miccolis Angelini, R. M., Dongiovanni, C., Pollastro, S., Fumarola, G., Di Carolo, M., Perrelli, D., Natale, P., & Faretra F. (2018). Use of biocontrol agents and botanicals in integrated management of Botrytis cinerea in table-grape vineyards. Journal of Agriculture and Horticulture Research, 74, 715-725. https://doi.org/10.1002/ps.4767
Shishatskaya, E., Menzianova, N., Zhila, N., Prudnikova, S., Volova, T., & Thomas S. (2018). Toxic effects of the fungicide tebuconazole on the root system of Fusarium-infected wheat plants. Plant Physiology and Biochemistry, 132, 400-407. https://doi.org/10.1016/j.plaphy.2018.09.025
Sun, H. Y., Zhu, Y. F., Liu, Y. Y., Deng, Y. Y., Li, W., Zhang, A. X., & Chen H. G. (2014). Evaluation of tebuconazole for the management of Fusarium head blight in China. Australasian Plant Pathology, 43, 631-638. https://doi.org/10.1007/s13313-014-0309-4
Vincent J. M. (1947). Distortion of fungal hyphae in the presence of certain inhibitors. Nature, 159:850. https://doi.org/10.1038/159850b0
Wang, B., Shen, Z., Zhang, F., Raza, W., Yuan, J., Huang, R., Ruan, Y., LI, R., & Shen Q. (2016). Bacillus amyloliquefaciens strain W19 can promote growth and yield and suppress Fusarium wilt in banana under greenhouse and field conditions. Pedosphere, 26(5), 733-744. https://doi.org/10.1016/S1002-0160(15)60083-2
Yanti, Y., Hamid, H., & Yaherwandi A. (2021). Biochemical characterizations of selected indigenous endophytic bacteria potential as growth promoters and biocontrol agents on tomato. IOP Conference Series: Earth and Environmental Science, 757, 012002. https://doi.org/10.1088/1755-1315/757/1/012002
Yin, Y. N., Liu, X., Li, B., & Ma Z. H. (2009). Characterization of sterol demethylation inhibitor-resistant isolates of Fusarium asiaticum and F.graminearum collected from wheat in China. Phytopathology, 99, 487-497 https://doi.org/10.1094/PHYTO-99-5-0487
Yu, Y., Jiang, C., Wang, C., Chen, L., Li, H., Xu, Q., & Guo J. (2017). An improved strategy for stable biocontrol agents selecting to control rice sheath blight caused by Rhizoctonia solani. Microbiological Research, 203, 1-9. https://doi.org/10.1016/j.micres.2017.05.006
Zhang, L. G., Jia, X. J., Chen, C. J., & Zhou M. G. (2013). College of characterization of carbendazim sensitivity and trichothecene chemotypes of Fusarium graminearum in Jiangsu Province of China. Physiological and Molecular Plant Pathology, 84, 53–60 https://doi.org/10.1016/j.pmpp.2013.07.005
Zhao, P., Quan, C., Wang, Y., Wang, J., & Fan S. (2013). Bacillus amyloliquefaciens Q-426 as a potential biocontrol agent against Fusarium oxysporum f. sp. spinaciae. Journal of Basic Microbiology, 54(5), 448-456. https://doi.org/10.1002/jobm.201200414
Zhu, J., Tan, T., Shen, A., Yang, X., Yu, Y., Gao, C., & Zeng L. (2020). Biocontrol potential of Bacillus subtilis IBFCBF-4 against Fusarium wilt of watermelon. Journal of Plant Pathology, 102, 433–441. https://doi.org/10.1007/s42161-019-00457-6
Published
2023-07-18
How to Cite
Oulmi, A., Bencheikh, A., Mamache, W., Gharzouli, A., Barkahoum Daichi, M., & Rouag, N. (2023). Study of synergistic effect of combined application of tebuconazole with two biocontrol agents for management of Fusarium crown rot in durum wheat. Revista De La Facultad De Agronomía De La Universidad Del Zulia, 40(3), e234025. Retrieved from https://www.produccioncientificaluz.org/index.php/agronomia/article/view/40560
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Section
Crop Production
Copyright (c) 2023 Abdelmalek Oulmi, Amor Bencheikh, Walid Mamache, Asma Gharzouli, Meriem Barkahoum Daichi, Noureddine Rouag
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
