Lipid Peroxidation, Proline Content and Soluble Sugars as indicators of Oxidative Stress Tolerance in Some Advanced Durum Wheat Lines (Triticum durum Desf.).
Keywords:
membrane damage, glyphosate, reactive oxygen species, tolerance
Abstract
Oxidative stress induced by glyphosate is a complex phenomenon caused by an imbalance between reactive oxygen species (ROS) and antioxidants in plants cells. The present research was carried out at the field crops institute, Agricultural Experimental Station of Setif (ITGC-AES), to assess the response of some durum wheat (Triticum Durum Desf.) lines exposed to oxidative stress induced by glyphosate herbicide. In the heading stage, a solution of 5 Mm of glyphosate was sprayed on flag leaves, and each measurement was taken 48 hours after the glyphosate application. Lipid peroxidation, free proline and soluble sugars were determined. The results indicated that oxidative stress increased the content of lipid peroxidation, proline, and soluble sugars in flag leaves. Analysis of variance revealed significant differences among the genotypes tested, the increase in the level of lipid peroxidation is much higher in advanced lines G5 and G3, in which lipid peroxidation and membrane damage are greater. Oxidative damage also increased the proline content in lines G3 and G4, and soluble sugars in line G5, which were showing a high tolerance to the oxidative stress induced.
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References
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Hanif, S., Saleem, M.F., Sarwar, M., Irshad, M., Shakoor, A., Wahid, M.A., Zaman Khan, H. (2021). Biochemically triggered heat and drought stress tolerance in rice by proline application. Journal of Plant Growth Regulation, 40, 305–312. https://doi.org/10.1007/s00344-020-10095-3.
Hemaprabha, G., Swapna, S., Leena Lavanya, D., Sajitha, B., Venkataramana S. (2013). Evaluation of drought tolerance potential of elite genotypes and progenies of sugarcane (Saccharum sp. hybrids). Sugar technology, (15)1, 9-16.https://doi.org/10.1007/s12355-012-0182-9.
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Karabulut, F., and Canakcı, S. (2021). Effects of Glyphosate Herbicide on Photosynthetic Pigments and Antioxidant Enzyme Activities in Corn (Zea mays L.) And Wheat (Triticuma estivum L.) Varieties. Journal of Physical Chemistry and Functional Materials, 4(2), 61-66. https://doi.org/10.54565/jphcfum.1004433.
Kavi Kishor, P.B., Suravajhala, P., Rathnagiri, P., and Sreenivasulu, N. (2022). Intriguing Role of Proline in Redox Potential Conferring High Temperature Stress Tolerance. Frontiers Plant Science, 13, 867531. https://doi.org/10.3389/fpls.2022.867531.
Ladoui, K., Mefti, M., and Benkherbache, N. (2020). Selection of drought tolerant genotypes of barley (Hordeum vulgare L.) through stress tolerance indices. Agrobiologia, 10, 1805-12. http://agrobiologia.net/online/selection-de-genotypes-dorge-hordeum-vulgare-l-tolerants-au-stress-hydrique-par-les-indices-de-tolerance-a-la-secheresse/
Langaro, A., Agostinetto, D., Ruchel, Q., Rodrigues Garcia, J., and TessariPerboni, L. (2020). Oxidative stress caused by the use of preemergent herbicides in rice crops. Revista CiênciaAgronômica, (48)2, 358-364. https://doi.org/10.5935/1806-6690.20170041.
Monneveux, P., Nemmar, M. (1986). Contribution to the study of drought resistance in soft wheat (Triticum aestivum L.) and durum wheat (Triticum durum Desf.): study of the accumulation of proline during the during the development cycle. Agronomy, 6 (6), 583-590. https://doi.org/10.1051/agro:19860611
Morales, M., and Munné-Bosch, S. (2019). Malondialdehyde: Facts and Artifacts. Plant Physiology, 180(3), 1246–1250. https://doi.org/10.1104/pp.19.00405.
Rajametov, S.N., Yang, E.Y., Cho, M.C., Chae, S.Y., Jeong, H.B. (2021). Heat-tolerant hot pepper exhibits constant photosynthesis via increased transpiration rate, high proline content and fast recovery in heat stress condition. Scientific Report, 12; 11(1), 14328. .https://doi.org/10.1038/s41598-021-93697-5.
Sami, F., Yusuf, M., Faizan, M., Faraz, A., Hayat, S. (2016). Role of sugars under abiotic stress. Plant Physiology et Biochemistry, 109, 54-61. https://doi.org/10.1016/j.plaphy.2016.09.005
Sergiev, I., Todorova, D., Shopova, E., Brankova, L., Jankauskienė, J., Jurkonienė, S., Gavelienė, V., and Mockeviciute., R. (2020). Assessment of synthetic auxin type compounds as potential modulators of herbicide action in Pisum sativum L. Biologia,75,1845–1853. https://doi.org/10.3390/agronomy10060793.
Sharma, A., Shahzad, B., Kumar, V., Kohli, S.K., Sidhu, G.P.S., Shreeya Bali, A., Handa, N., Kapoor, D., Bhardwaj, R., and Zheng, B. (2019). Phytohormones Regulate Accumulation of Osmolytes Under Abiotic Stress. Biomolecules, 9(7), 285. https://doi.org/10.3390/biom9070285.
Sharma, P., Jha, A.B., Dubey, R.S. (2016). Oxidative stress and antioxidative defense systems in plants growing under abiotic stresses. Handbook of Plant and Crop Stress, p 109–158. https://doi.org/10.3390/antiox9080681.
Shopova, E., Brankova, L., Katerova, Z., Dimitrova, L., Todorova, D., Sergiev, I., and Talaat, N.B. (2021). Salicylic Acid Pretreatment Modulates Wheat Responses to Glyphosate. Crops, 1(2), 88–96. https://doi.org/10.3390/crops1020009.
Singh, R., Rathore, D. (2017). Oxidative stress defence responses of wheat (Triticuma estivum L.) and chilli (Capsicum annum L.) cultivars grown under textile effluent fertilization, Plant Physiology and Biochemistry. 123, 342-358. https://doi.org/10.1016/j.plaphy.2017.12.027.
Soares, C., Pereira, R., Spormann, S., Fidalgo, F. (2019). Is soil contamination by a glyphosate commercial formulation truly harmless to non-target plants-Evaluation of oxidative damage and antioxidant responses in tomato. Environnemental Pollution., 247, 256–265. https://doi.org/10.1016/j.envpol.2019.01.063.
Spormann, S., Soares, C., Fidalgo, F. (2019). Salicylic acid alleviates glyphosate-induced oxidative stress in Hordeum vulgare L. Journal of Environmental Management, 241, 226–23. https://doi.org/10.3390/crops1020009.
Staub, A.M. (1963). Extraction, identification and assays of carbohydrates in organ extracts and bacterial bodies. In: Laboratory techniques, volumes 1 and 2, Masson, Paris, 1307-1366.
Tulkova, E., Kabashinikova, L. (2021). Malondialdehyde content in the leaves of smallleaved linden tiliacordata and Norway maple acer platanoides under the influence of volatile organic compounds. Plant Biosystems, 156(2), 619-627 https://doi.org/10.1080/11263504.2021.1897701.
Xynias, I., Mylonas, I., Korpetis, G., Ninou, E., Tsaballa, A., Avdikos, I., and Mavromatis, G. (2020). Durum Wheat Breeding in the Mediterranean Region: Current Status and Future Prospects. Agronomy, 10, 432. https://doi.org/10.3390/agronomy10030432.
Zhang, L., Chen, L., Zhang, H., Ren, Z., and Luo, P. (2013). Effects of paraquat-induced oxidative stress on antioxidants and chlorophyll fluorescence in Stay-green wheat (Triticum aestivum L.) Flag leaves. Bangladesh Journal of Botany, 42(2), 239-245. https://doi.org/10.3329/bjb.v42i2.18025.
Zhao, L., Xie, L., Huang, J., Su, Y., and Zhang, C. (2020). Proper Glyphosate Application at Post-anthesis Lowers Grain Moisture Content at Harvest and Reallocates Non-structural Carbohydrates in Maize. Frontiers in plant sciences, 11: 580883. https://doi.org/10.3389/fpls.2020.580883.
Bao, A.K., Wang, S.M., Wu, G.Q., Xi, J.J., Zhang, J.L., Wang, C.M. (2009). Overexpression of the Arabidopsis H+-PPase enhanced resistance to salt and drought stress in transgenic alfalfa (Medicago sativa L.). Plant Science, 176, 232–240. https://doi.org/10.1016/j.plantsci.2008.10.009.
Ben M’Barek, S., Laribi, M., Kouki, H., Castillo, D., Araar, C., Nefzaoui, M., Ammar, K., Saint-Pierre, C., Yahyaoui, A.H. (2022). Phenotyping Mediterranean Durum Wheat Landraces for Resistance to Zymoseptoria tritici in Tunisia. Genes, 13, 355. https://doi.org/10.3390/genes13020355
Bouchemal, K., Bouldjadj, R., Belbekri, M.N., Ykhlef, N., and Djekoun, A. (2016). Differences in antioxidant enzyme activities and oxidative markers in ten wheat (Triticumdurum Desf.) genotypes in response to drought, heat and paraquat stress. Archives of Agronomy and Soil Science, (63) 5. https://doi.org/10.1080/03650340.2016.1235267.
Chaki, M., Begara-Morales, J.C., Barroso, J.B. (2020). Oxidative Stress in Plants. Antioxidants, 9, 481. https://doi.org/10.3390/antiox9060481.
Chauhan, J., Srivastava, J.P., KumarSinghal, R., Soufan, W., KumarDadarwal, B. (2022). Alterations of Oxidative Stress Indicators, Antioxidant Enzymes, Soluble Sugars, and Amino Acids in Mustard [Brassica juncea (L.) Czern and Coss.] in Response to Varying Sowing Time, and Field Temperature. Frontiers in plant science,13: 875009. https://doi.org/10.3389/fpls.2022.875009.
Elewa, T.A., Sadak, M.S., Saad, A.M. (2017). Proline treatment improves physiological responses in quinoa plants under drought stress. Bioscience Research, 14:21–33.
Fernández-Escalada, M., González, A., Monreal, M., Royuela, M., and Zabalza, A. (2019). Physiological performance of glyphosate and imazamox mixtures on Amaranthuspalmeri sensitive and resistant to glyphosate. Scientific Reports, 9,18225. https://doi.org/10.1038/s41598-019-54642-9.
Gomes, M.P., Le Manach, S.G., Hénault-Ethier, L., Labrecque, M., and Juneau, P. (2017). Glyphosate-Dependent Inhibition of Photosynthesis in Willow. Frontiers in plant science, 8, 207. https://doi.org/10.3389/fpls.2017.00207.
Gomes, M.P., Smedbol, E., Chalifour, A., Hénault-Ethier, L., Labrecque, M., Lepage, L., Lucotte, M., and Juneau, P. (2014). Alteration of plant physiology by glyphosate and its by-product aminomethylphosphonic acid: an overview. Journal of Experimental Botany, (65)17. https://doi.org/10.1093/jxb/eru269.
Hanif, S., Saleem, M.F., Sarwar, M., Irshad, M., Shakoor, A., Wahid, M.A., Zaman Khan, H. (2021). Biochemically triggered heat and drought stress tolerance in rice by proline application. Journal of Plant Growth Regulation, 40, 305–312. https://doi.org/10.1007/s00344-020-10095-3.
Hemaprabha, G., Swapna, S., Leena Lavanya, D., Sajitha, B., Venkataramana S. (2013). Evaluation of drought tolerance potential of elite genotypes and progenies of sugarcane (Saccharum sp. hybrids). Sugar technology, (15)1, 9-16.https://doi.org/10.1007/s12355-012-0182-9.
Hosseinifard, M., Stefaniak, S., GhorbaniJavid, M., Soltani, E., Wojtyla, L., and Garnczarska, M. (2022). Contribution of Exogenous Proline to Abiotic Stresses Tolerance in Plants: A Review. International journal of Molecular science, 23(9), 5186. https://doi.org/10.3390/ijms23095186.
Karabulut, F., and Canakcı, S. (2021). Effects of Glyphosate Herbicide on Photosynthetic Pigments and Antioxidant Enzyme Activities in Corn (Zea mays L.) And Wheat (Triticuma estivum L.) Varieties. Journal of Physical Chemistry and Functional Materials, 4(2), 61-66. https://doi.org/10.54565/jphcfum.1004433.
Kavi Kishor, P.B., Suravajhala, P., Rathnagiri, P., and Sreenivasulu, N. (2022). Intriguing Role of Proline in Redox Potential Conferring High Temperature Stress Tolerance. Frontiers Plant Science, 13, 867531. https://doi.org/10.3389/fpls.2022.867531.
Ladoui, K., Mefti, M., and Benkherbache, N. (2020). Selection of drought tolerant genotypes of barley (Hordeum vulgare L.) through stress tolerance indices. Agrobiologia, 10, 1805-12. http://agrobiologia.net/online/selection-de-genotypes-dorge-hordeum-vulgare-l-tolerants-au-stress-hydrique-par-les-indices-de-tolerance-a-la-secheresse/
Langaro, A., Agostinetto, D., Ruchel, Q., Rodrigues Garcia, J., and TessariPerboni, L. (2020). Oxidative stress caused by the use of preemergent herbicides in rice crops. Revista CiênciaAgronômica, (48)2, 358-364. https://doi.org/10.5935/1806-6690.20170041.
Monneveux, P., Nemmar, M. (1986). Contribution to the study of drought resistance in soft wheat (Triticum aestivum L.) and durum wheat (Triticum durum Desf.): study of the accumulation of proline during the during the development cycle. Agronomy, 6 (6), 583-590. https://doi.org/10.1051/agro:19860611
Morales, M., and Munné-Bosch, S. (2019). Malondialdehyde: Facts and Artifacts. Plant Physiology, 180(3), 1246–1250. https://doi.org/10.1104/pp.19.00405.
Rajametov, S.N., Yang, E.Y., Cho, M.C., Chae, S.Y., Jeong, H.B. (2021). Heat-tolerant hot pepper exhibits constant photosynthesis via increased transpiration rate, high proline content and fast recovery in heat stress condition. Scientific Report, 12; 11(1), 14328. .https://doi.org/10.1038/s41598-021-93697-5.
Sami, F., Yusuf, M., Faizan, M., Faraz, A., Hayat, S. (2016). Role of sugars under abiotic stress. Plant Physiology et Biochemistry, 109, 54-61. https://doi.org/10.1016/j.plaphy.2016.09.005
Sergiev, I., Todorova, D., Shopova, E., Brankova, L., Jankauskienė, J., Jurkonienė, S., Gavelienė, V., and Mockeviciute., R. (2020). Assessment of synthetic auxin type compounds as potential modulators of herbicide action in Pisum sativum L. Biologia,75,1845–1853. https://doi.org/10.3390/agronomy10060793.
Sharma, A., Shahzad, B., Kumar, V., Kohli, S.K., Sidhu, G.P.S., Shreeya Bali, A., Handa, N., Kapoor, D., Bhardwaj, R., and Zheng, B. (2019). Phytohormones Regulate Accumulation of Osmolytes Under Abiotic Stress. Biomolecules, 9(7), 285. https://doi.org/10.3390/biom9070285.
Sharma, P., Jha, A.B., Dubey, R.S. (2016). Oxidative stress and antioxidative defense systems in plants growing under abiotic stresses. Handbook of Plant and Crop Stress, p 109–158. https://doi.org/10.3390/antiox9080681.
Shopova, E., Brankova, L., Katerova, Z., Dimitrova, L., Todorova, D., Sergiev, I., and Talaat, N.B. (2021). Salicylic Acid Pretreatment Modulates Wheat Responses to Glyphosate. Crops, 1(2), 88–96. https://doi.org/10.3390/crops1020009.
Singh, R., Rathore, D. (2017). Oxidative stress defence responses of wheat (Triticuma estivum L.) and chilli (Capsicum annum L.) cultivars grown under textile effluent fertilization, Plant Physiology and Biochemistry. 123, 342-358. https://doi.org/10.1016/j.plaphy.2017.12.027.
Soares, C., Pereira, R., Spormann, S., Fidalgo, F. (2019). Is soil contamination by a glyphosate commercial formulation truly harmless to non-target plants-Evaluation of oxidative damage and antioxidant responses in tomato. Environnemental Pollution., 247, 256–265. https://doi.org/10.1016/j.envpol.2019.01.063.
Spormann, S., Soares, C., Fidalgo, F. (2019). Salicylic acid alleviates glyphosate-induced oxidative stress in Hordeum vulgare L. Journal of Environmental Management, 241, 226–23. https://doi.org/10.3390/crops1020009.
Staub, A.M. (1963). Extraction, identification and assays of carbohydrates in organ extracts and bacterial bodies. In: Laboratory techniques, volumes 1 and 2, Masson, Paris, 1307-1366.
Tulkova, E., Kabashinikova, L. (2021). Malondialdehyde content in the leaves of smallleaved linden tiliacordata and Norway maple acer platanoides under the influence of volatile organic compounds. Plant Biosystems, 156(2), 619-627 https://doi.org/10.1080/11263504.2021.1897701.
Xynias, I., Mylonas, I., Korpetis, G., Ninou, E., Tsaballa, A., Avdikos, I., and Mavromatis, G. (2020). Durum Wheat Breeding in the Mediterranean Region: Current Status and Future Prospects. Agronomy, 10, 432. https://doi.org/10.3390/agronomy10030432.
Zhang, L., Chen, L., Zhang, H., Ren, Z., and Luo, P. (2013). Effects of paraquat-induced oxidative stress on antioxidants and chlorophyll fluorescence in Stay-green wheat (Triticum aestivum L.) Flag leaves. Bangladesh Journal of Botany, 42(2), 239-245. https://doi.org/10.3329/bjb.v42i2.18025.
Zhao, L., Xie, L., Huang, J., Su, Y., and Zhang, C. (2020). Proper Glyphosate Application at Post-anthesis Lowers Grain Moisture Content at Harvest and Reallocates Non-structural Carbohydrates in Maize. Frontiers in plant sciences, 11: 580883. https://doi.org/10.3389/fpls.2020.580883.
Published
2023-05-23
How to Cite
Oulmi, A., Benkadja, S., Guendouz, A., Frih, B., Mehanni, A., & Selloum, S. (2023). Lipid Peroxidation, Proline Content and Soluble Sugars as indicators of Oxidative Stress Tolerance in Some Advanced Durum Wheat Lines (Triticum durum Desf.). Revista De La Facultad De Agronomía De La Universidad Del Zulia, 40(2), e234018. Retrieved from https://www.produccioncientificaluz.org/index.php/agronomia/article/view/40196
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Section
Crop Production
Copyright (c) 2023 Abdelmalek Oulmi, Sarah Benkadja, Ali Guendouz, Benalia Frih, Amor Mehanni and Samir Selloum
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
