Bioindicators of water quality with different riparian cover in the Guapara micro watershed, Ecuador
Keywords:
Aquatic macroinvertebrates, Land use, Biological indices
Abstract
Agricultural activities produce changes in the margins of the stream banks, causing disturbances in water quality, and in the assemblage of aquatic insect communities. The objective of this research is to evaluate the changes in the structure and composition of the aquatic macroinvertebrate community, and physicochemical variables in relation to the land use of the Guapara river, Cotopaxi province, Ecuador. Samplings were conducted during the rainy season (December to February 2018-2019) in three streams with different riparian cover: agricultural, native forest, and forest plantations, where samples of aquatic insects and physicochemical parameters were collected in situ. A total of 461 aquatic insects corresponding to 7 orders and 25 families were collected. In general, the highest abundance in order and family was presented by Ephemeroptera with 34 %, and Leptophlebiidae with 21 % respectively. According to the Ephemeroptera, Plecoptera Trichoptera (EPT) index, the highest ecological condition was found in streams with native forest cover, and forest plantations respectively, and decreased in the stream with agricultural cover; while BMWP-Cr indicated water contamination in the three streams. Hydrobiosidae (Trichoptera) were associated with high turbidity values; while Gerridae (Hemiptera) were associated with high dissolved oxygen concentrations; in addition, Ptilodactylidae (Coleoptera) preferred the Q1-CA, and Q3-CF conditions, while Leptophlebiidae (Ephemeroptera) were associated with the stream of native forest. A negative influence of agricultural activity on the physicochemical parameters of the water and on the structure of the aquatic insect community assemblage was evidenced.
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References
Alomía, J., Iannacone, J. A., Alvariño, L., & Ventura, K. (2017). Macroinvertebrados Bentónicos Para Evaluar La Calidad De Las Aguas De La Cuenca Alta Del Río Huallaga, Perú. The Biologist, 1(2), 65–84. https://doi.org/10.24039/rtb2017151144
Blanchet, F. G., Legendre, P., & Borcard, D. (2008). Forward selection of explanatory variables. Ecology, 89(9), 2623–2632. https://doi.org/10.1890/07-0986.1
Bojsen, B. H., and Jacobsen, D. (2003). Effects of deforestation on macroinvertebrate diversity and assemblage structure in Ecuadorian Amazon streams. Archiv Fur Hydrobiologie, 158(3), 317–342.
Bonada, N., Prat, N., Resh, V. H., & Statzner, B. (2006). Developments in aquatic insect biomonitoring: a comparative analysis of recent approaches. Annu. Rev. Entomol., 51, 495–523.
Capparelli, M. V., Molinero, J., Moulatlet, G. M., Barrado, M., Prado-Alcívar, S., Cabrera, M., Gimiliani, G., Ñacato, C., Pinos-Velez, V., & Cipriani-Avila, I. (2021). Microplastics in rivers and coastal waters of the province of Esmeraldas, Ecuador. Marine Pollution Bulletin, 173. https://doi.org/10.1016/j.marpolbul.2021.113067
Damanik-Ambarita, M. N., Lock, K., Boets, P., Everaert, G., Nguyen, T. H. T., Forio, M. A. E., Musonge, P. L. S., Suhareva, N., Bennetsen, E., Landuyt, D., Dominguez-Granda, L., & Goethals, P. L. M. (2016). Ecological water quality analysis of the Guayas river basin (Ecuador) based on macroinvertebrates indices. Limnologica, 57, 27–59. https://doi.org/10.1016/j.limno.2016.01.001
Deknock, A., De Troyer, N., Houbraken, M., Dominguez-Granda, L., Nolivos, I., Van Echelpoel, W., Forio, M. A. E., Spanoghe, P., & Goethals, P. (2019). Distribution of agricultural pesticides in the freshwater environment of the Guayas river basin (Ecuador). Science of The Total Environment, 646, 996–1008. https://doi.org/10.1016/j.scitotenv.2018.07.185
Domínguez, E., and Fernández, H. R. (2009). Macroinvertebrados bentónicos sudamericanos Sistemática y biología. Fundación Miguel Lillo.
Forio, M. A. E., Van Echelpoel, W., Dominguez-Granda, L., Mereta, S. T., Ambelu, A., Hoang, T. H., Boets, P., & Goethals, P. L. M. (2016). Analysing the effects of water quality on the occurrence of freshwater macroinvertebrate taxa among tropical river basins from different continents. Ai Communications, 29(6), 665–685.
Galeano-Rendón, E., and Mancera-Rodríguez, N. J. (2018). Efectos de la deforestación sobre la diversidad y la estructura del ensamblaje de macroinvertebrados en cuatro quebradas Andinas en Colombia. Revista de Biología Tropical, 66(4), 1721–1740.
Gozlan, R. E., Karimov, B. K., Zadereev, E., Kuznetsova, D., & Brucet, S. (2019). Status, trends, and future dynamics of freshwater ecosystems in Europe and Central Asia. Inland Waters, 9(1), 78–94. https://doi.org/10.1080/20442041.2018.1510271
Guerrero, N., Díaz, M., Urdánigo, J., Tayhing, C., Guerrero, V., & Yepez, Á. (2017). Uso de suelo y su influencia en la calidad del agua de la microcuenca El Sapanal, Ecuador. Revista Cubana de Ciencias Biológicas, 5(2), 1–11. http://www.rccb.uh.cu/index.php/RCCB/article/view/156/265
Gutiérrez-Fonseca, P. E., and Lorion, C. M. (2014). Application of the BMWP-Costa Rica biotic index in aquatic biomonitoring: Sensitivity to collection method and sampling intensity. Revista de Biologia Tropical, 62(April), 275–289. https://doi.org/10.15517/rbt.v62i0.15792
He, S., Soininen, J., Chen, K., & Wang, B. (2020). Environmental factors override dispersal-related factors in shaping diatom and macroinvertebrate communities within stream networks in China. Frontiers in Ecology and Evolution, 8, 141.
Hill, M. O., and Gauch, H. G. (1980). Detrended correspondence analysis: An improved ordination technique. Vegetatio, 42(1), 47–58. https://doi.org/10.1007/BF00048870
Holdridge, L. (1978). Ecología basada en zonas de vida. Instituto Interamericano de Ciencias Agrícolas (IICA). http://repositorio.iica.int/handle/11324/7936
Jandry, J., Brulin, M., Parinet, B., & Grandjean, F. (2014). Ephemeroptera communities as bioindicators of the suitability of headwater streams for restocking with white-clawed crayfish, Austropotamobius pallipes. Ecological Indicators, 46, 560–565.
Malaj, E., Von Der Ohe, P. C., Grote, M., Kühne, R., Mondy, C. P., Usseglio-Polatera, P., Brack, W., & Schäfer, R. B. (2014). Organic chemicals jeopardize the health of freshwater ecosystems on the continental scale. Proceedings of the National Academy of Sciences of the United States of America, 111(26), 9549–9554. https://doi.org/10.1073/pnas.1321082111
Mena-Rivera, L., Vásquez-Bolaños, O., Gómez-Castro, C., Fonseca-Sánchez, A., Rodríguez-Rodríguez, A., & Sánchez-Gutiérrez, R. (2018). Ecosystemic assessment of surface water quality in the Virilla river: towards sanitation processes in Costa Rica. Water, 10(7), 845.
Meza, M., Rubio, J., Días, A., & Walteros, L. (2012). Calidad de agua y composición de macroinvertebrados acuáticos en la subcuenca alta del río Chinchiná. Water quality and composition of aquatic macroinvertebrates in the subwatershed of river Chinchiná. Caldasia. Caldasia, 34(2), 443–456. http://www.icn.unal.edu.co/
MINAE. (2007). Reglamento para la Evaluación y Clasificación de la Calidad de los Cuerpos de Aguas Superficiales. 178. www.cimar.ucr.ac.cr/PDFS/Reglamento Evaluacion y Clasifica-cion_Calidad_Agua_Superficial.pdf
Morelli, E., and Verdi, A. (2014). Diversidad de macroinvertebrados acuáticos en cursos de agua dulce con vegetación ribereña nativa de Uruguay. Revista Mexicana de Biodiversidad, 85(4), 1160–1170. https://doi.org/10.7550/rmb.45419
Mosquera-Restrepo, D., and Peña-Salamanca, E. J. (2019). “Ensamblaje” de macroinvertebrados acuáticos y su relación con variables fisicoquímicas en un río de montaña en Colombia. Revista de Biología Tropical, 67(6), 1235–1246.
Murillo, S. A., Mendoza, A., Restrepo, E. S., & Rodríguez, M. Á. (2018). Utilización de macroinvertebrados acuáticos como herramienta para determinar la calidad del agua en la quebrada Santo Tomás, municipio de Pensilvania, Colombia. Revista de La Academia Colombiana de Ciencias Exactas, Físicas y Naturales, 42(164), 212–220. https://doi.org/10.18257/raccefyn.655
Nuñez, J. C., and Fragoso-Castilla, P. J. (2019). Uso de macroinvertebrados acuáticos como bioindicadores de contaminación del agua de la Ciénaga Mata de Palma (Colombia). Información Tecnológica, 30(5), 319–330.
Oksanen, J., Blanchet, F. G., Friendly, M., Kindt, R., Legendre, P., McGlinn, D., Minchin, P. R., O’Hara, R. B., Simpson, G. L., Solymos, P., Stevens, M. H. H., Szoecs, E., & Wagner, H. (2015). Vegan: Community Ecology Package (R package version 2. 3-0). https://cran.r-project.org/package=vegan
Pérez-Rodríguez, C., Manjarres-Pinzón, G., & Tamaris-Turizo, C. . (2021). Insectos acuáticos asociados a arroyos de la Serranía de La Macuira - La Guajira, Colombia. Revista U.D.C.A Actualidad & Divulgación Científica, 24(1). https://doi.org/10.31910/rudca.v24.n1.2021.1941
Pimentel, D., and Burgess, M. (2013). Soil erosion threatens food production. Agriculture (Switzerland), 3(3), 443–463. https://doi.org/10.3390/agriculture3030443
R Core Team. (2015). A language and environment for statistical computing. R Foundation for Statistical Computing.
Rojas-Múnera, D. M., Feijoo-Martínez, A., Molina-Rico, L. J., Zúñiga, M. C., & Quintero, H. (2021). Differential impact of altitude and a plantain cultivation system on soil macroinvertebrates in the Colombian Coffee Region. Applied Soil Ecology, 164, 103931. https://doi.org/https://doi.org/10.1016/j.apsoil.2021.103931
Roldán, G. (2003). Bioindicación de la calidad del agua en Colombia: propuesta para el uso del método BMWP Col (Universidad de Antioquia (ed.)). Editorial Universidad de Antioquia. https://books.google.com.ec/books?id=ZEjgIKZTF2UC
Rosado Yépez, Á., Bolívar Yépez, Á., Urdánigo Zambrano, J., Morales Cabezas, D. C., Guerrero Chuez, N. M., and Tayhing Cajas, C. C. (2017). Macroinvertebrados acuáticos como indicadores de calidad hídrica en áreas de descargas residuales al río Quevedo, Ecuador. Ciencias Tecn UTEQ, 10(1), 27–34.
Stark, J. D. (1998). SQMCI: A biotic index for freshwater macroinvertebrate coded‐abundance data. New Zealand Journal of Marine and Freshwater Research, 32(1), 55–66.
Stehle, S., and Schulz, R. (2015). Agricultural insecticides threaten surface waters at the global scale. Proceedings of the National Academy of Sciences of the United States of America, 112(18), 5750–5755. https://doi.org/10.1073/pnas.1500232112
Tampo, L., Kaboré, I., Alhassan, E. H., Ouéda, A., Bawa, L. M., & Djaneye-Boundjou, G. (2021). Benthic macroinvertebrates as ecological indicators: their sensitivity to the water quality and human disturbances in a tropical river. Frontiers in Water, 3, 662765.
Urdanigo, J. P., Díaz Ponce, M., Tay-Hing Cajas, C., Sánchez Fonseca, C., Yong Benitez, R., Armijo Albán, K., Guerrero Chúez, N., & Mancera-Rodríguez, N. J. (2019a). Diversidad de macroinvertebrados acuáticos en quebradas con diferente cobertura ribereña en del bosque Protector Murocomba, cantón Valencia, Ecuador. Revista de Biología Tropical, 67(4), 861–878. https://doi.org/10.15517/rbt.v67i4.35190
Urdanigo, J. P., Díaz Ponce, M., Tay-Hing Cajas, C., Sánchez Fonseca, C., Yong Benitez, R., Armijo Albán, K., Guerrero Chúez, N., & Mancera-Rodríguez, N. J. (2019b). Diversidad de macroinvertebrados acuáticos en quebradas con diferente cobertura ribereña en del bosque Protector Murocomba, cantón Valencia, Ecuador. Revista de Biología Tropical, 67(4), 861–878. https://doi.org/10.15517/rbt.v67i4.35190
Zapata, D., Oleas, N. H., Páez-Vacas, M., & Tobes, I. (2021). Water Quality Assessment of the Cutuchi River Basin (Ecuador): A Review of Technical Documents. IOP Conference Series: Earth and Environmental Science, 690(1), 12058. https://doi.org/10.1088/1755-1315/690/1/012058
Blanchet, F. G., Legendre, P., & Borcard, D. (2008). Forward selection of explanatory variables. Ecology, 89(9), 2623–2632. https://doi.org/10.1890/07-0986.1
Bojsen, B. H., and Jacobsen, D. (2003). Effects of deforestation on macroinvertebrate diversity and assemblage structure in Ecuadorian Amazon streams. Archiv Fur Hydrobiologie, 158(3), 317–342.
Bonada, N., Prat, N., Resh, V. H., & Statzner, B. (2006). Developments in aquatic insect biomonitoring: a comparative analysis of recent approaches. Annu. Rev. Entomol., 51, 495–523.
Capparelli, M. V., Molinero, J., Moulatlet, G. M., Barrado, M., Prado-Alcívar, S., Cabrera, M., Gimiliani, G., Ñacato, C., Pinos-Velez, V., & Cipriani-Avila, I. (2021). Microplastics in rivers and coastal waters of the province of Esmeraldas, Ecuador. Marine Pollution Bulletin, 173. https://doi.org/10.1016/j.marpolbul.2021.113067
Damanik-Ambarita, M. N., Lock, K., Boets, P., Everaert, G., Nguyen, T. H. T., Forio, M. A. E., Musonge, P. L. S., Suhareva, N., Bennetsen, E., Landuyt, D., Dominguez-Granda, L., & Goethals, P. L. M. (2016). Ecological water quality analysis of the Guayas river basin (Ecuador) based on macroinvertebrates indices. Limnologica, 57, 27–59. https://doi.org/10.1016/j.limno.2016.01.001
Deknock, A., De Troyer, N., Houbraken, M., Dominguez-Granda, L., Nolivos, I., Van Echelpoel, W., Forio, M. A. E., Spanoghe, P., & Goethals, P. (2019). Distribution of agricultural pesticides in the freshwater environment of the Guayas river basin (Ecuador). Science of The Total Environment, 646, 996–1008. https://doi.org/10.1016/j.scitotenv.2018.07.185
Domínguez, E., and Fernández, H. R. (2009). Macroinvertebrados bentónicos sudamericanos Sistemática y biología. Fundación Miguel Lillo.
Forio, M. A. E., Van Echelpoel, W., Dominguez-Granda, L., Mereta, S. T., Ambelu, A., Hoang, T. H., Boets, P., & Goethals, P. L. M. (2016). Analysing the effects of water quality on the occurrence of freshwater macroinvertebrate taxa among tropical river basins from different continents. Ai Communications, 29(6), 665–685.
Galeano-Rendón, E., and Mancera-Rodríguez, N. J. (2018). Efectos de la deforestación sobre la diversidad y la estructura del ensamblaje de macroinvertebrados en cuatro quebradas Andinas en Colombia. Revista de Biología Tropical, 66(4), 1721–1740.
Gozlan, R. E., Karimov, B. K., Zadereev, E., Kuznetsova, D., & Brucet, S. (2019). Status, trends, and future dynamics of freshwater ecosystems in Europe and Central Asia. Inland Waters, 9(1), 78–94. https://doi.org/10.1080/20442041.2018.1510271
Guerrero, N., Díaz, M., Urdánigo, J., Tayhing, C., Guerrero, V., & Yepez, Á. (2017). Uso de suelo y su influencia en la calidad del agua de la microcuenca El Sapanal, Ecuador. Revista Cubana de Ciencias Biológicas, 5(2), 1–11. http://www.rccb.uh.cu/index.php/RCCB/article/view/156/265
Gutiérrez-Fonseca, P. E., and Lorion, C. M. (2014). Application of the BMWP-Costa Rica biotic index in aquatic biomonitoring: Sensitivity to collection method and sampling intensity. Revista de Biologia Tropical, 62(April), 275–289. https://doi.org/10.15517/rbt.v62i0.15792
He, S., Soininen, J., Chen, K., & Wang, B. (2020). Environmental factors override dispersal-related factors in shaping diatom and macroinvertebrate communities within stream networks in China. Frontiers in Ecology and Evolution, 8, 141.
Hill, M. O., and Gauch, H. G. (1980). Detrended correspondence analysis: An improved ordination technique. Vegetatio, 42(1), 47–58. https://doi.org/10.1007/BF00048870
Holdridge, L. (1978). Ecología basada en zonas de vida. Instituto Interamericano de Ciencias Agrícolas (IICA). http://repositorio.iica.int/handle/11324/7936
Jandry, J., Brulin, M., Parinet, B., & Grandjean, F. (2014). Ephemeroptera communities as bioindicators of the suitability of headwater streams for restocking with white-clawed crayfish, Austropotamobius pallipes. Ecological Indicators, 46, 560–565.
Malaj, E., Von Der Ohe, P. C., Grote, M., Kühne, R., Mondy, C. P., Usseglio-Polatera, P., Brack, W., & Schäfer, R. B. (2014). Organic chemicals jeopardize the health of freshwater ecosystems on the continental scale. Proceedings of the National Academy of Sciences of the United States of America, 111(26), 9549–9554. https://doi.org/10.1073/pnas.1321082111
Mena-Rivera, L., Vásquez-Bolaños, O., Gómez-Castro, C., Fonseca-Sánchez, A., Rodríguez-Rodríguez, A., & Sánchez-Gutiérrez, R. (2018). Ecosystemic assessment of surface water quality in the Virilla river: towards sanitation processes in Costa Rica. Water, 10(7), 845.
Meza, M., Rubio, J., Días, A., & Walteros, L. (2012). Calidad de agua y composición de macroinvertebrados acuáticos en la subcuenca alta del río Chinchiná. Water quality and composition of aquatic macroinvertebrates in the subwatershed of river Chinchiná. Caldasia. Caldasia, 34(2), 443–456. http://www.icn.unal.edu.co/
MINAE. (2007). Reglamento para la Evaluación y Clasificación de la Calidad de los Cuerpos de Aguas Superficiales. 178. www.cimar.ucr.ac.cr/PDFS/Reglamento Evaluacion y Clasifica-cion_Calidad_Agua_Superficial.pdf
Morelli, E., and Verdi, A. (2014). Diversidad de macroinvertebrados acuáticos en cursos de agua dulce con vegetación ribereña nativa de Uruguay. Revista Mexicana de Biodiversidad, 85(4), 1160–1170. https://doi.org/10.7550/rmb.45419
Mosquera-Restrepo, D., and Peña-Salamanca, E. J. (2019). “Ensamblaje” de macroinvertebrados acuáticos y su relación con variables fisicoquímicas en un río de montaña en Colombia. Revista de Biología Tropical, 67(6), 1235–1246.
Murillo, S. A., Mendoza, A., Restrepo, E. S., & Rodríguez, M. Á. (2018). Utilización de macroinvertebrados acuáticos como herramienta para determinar la calidad del agua en la quebrada Santo Tomás, municipio de Pensilvania, Colombia. Revista de La Academia Colombiana de Ciencias Exactas, Físicas y Naturales, 42(164), 212–220. https://doi.org/10.18257/raccefyn.655
Nuñez, J. C., and Fragoso-Castilla, P. J. (2019). Uso de macroinvertebrados acuáticos como bioindicadores de contaminación del agua de la Ciénaga Mata de Palma (Colombia). Información Tecnológica, 30(5), 319–330.
Oksanen, J., Blanchet, F. G., Friendly, M., Kindt, R., Legendre, P., McGlinn, D., Minchin, P. R., O’Hara, R. B., Simpson, G. L., Solymos, P., Stevens, M. H. H., Szoecs, E., & Wagner, H. (2015). Vegan: Community Ecology Package (R package version 2. 3-0). https://cran.r-project.org/package=vegan
Pérez-Rodríguez, C., Manjarres-Pinzón, G., & Tamaris-Turizo, C. . (2021). Insectos acuáticos asociados a arroyos de la Serranía de La Macuira - La Guajira, Colombia. Revista U.D.C.A Actualidad & Divulgación Científica, 24(1). https://doi.org/10.31910/rudca.v24.n1.2021.1941
Pimentel, D., and Burgess, M. (2013). Soil erosion threatens food production. Agriculture (Switzerland), 3(3), 443–463. https://doi.org/10.3390/agriculture3030443
R Core Team. (2015). A language and environment for statistical computing. R Foundation for Statistical Computing.
Rojas-Múnera, D. M., Feijoo-Martínez, A., Molina-Rico, L. J., Zúñiga, M. C., & Quintero, H. (2021). Differential impact of altitude and a plantain cultivation system on soil macroinvertebrates in the Colombian Coffee Region. Applied Soil Ecology, 164, 103931. https://doi.org/https://doi.org/10.1016/j.apsoil.2021.103931
Roldán, G. (2003). Bioindicación de la calidad del agua en Colombia: propuesta para el uso del método BMWP Col (Universidad de Antioquia (ed.)). Editorial Universidad de Antioquia. https://books.google.com.ec/books?id=ZEjgIKZTF2UC
Rosado Yépez, Á., Bolívar Yépez, Á., Urdánigo Zambrano, J., Morales Cabezas, D. C., Guerrero Chuez, N. M., and Tayhing Cajas, C. C. (2017). Macroinvertebrados acuáticos como indicadores de calidad hídrica en áreas de descargas residuales al río Quevedo, Ecuador. Ciencias Tecn UTEQ, 10(1), 27–34.
Stark, J. D. (1998). SQMCI: A biotic index for freshwater macroinvertebrate coded‐abundance data. New Zealand Journal of Marine and Freshwater Research, 32(1), 55–66.
Stehle, S., and Schulz, R. (2015). Agricultural insecticides threaten surface waters at the global scale. Proceedings of the National Academy of Sciences of the United States of America, 112(18), 5750–5755. https://doi.org/10.1073/pnas.1500232112
Tampo, L., Kaboré, I., Alhassan, E. H., Ouéda, A., Bawa, L. M., & Djaneye-Boundjou, G. (2021). Benthic macroinvertebrates as ecological indicators: their sensitivity to the water quality and human disturbances in a tropical river. Frontiers in Water, 3, 662765.
Urdanigo, J. P., Díaz Ponce, M., Tay-Hing Cajas, C., Sánchez Fonseca, C., Yong Benitez, R., Armijo Albán, K., Guerrero Chúez, N., & Mancera-Rodríguez, N. J. (2019a). Diversidad de macroinvertebrados acuáticos en quebradas con diferente cobertura ribereña en del bosque Protector Murocomba, cantón Valencia, Ecuador. Revista de Biología Tropical, 67(4), 861–878. https://doi.org/10.15517/rbt.v67i4.35190
Urdanigo, J. P., Díaz Ponce, M., Tay-Hing Cajas, C., Sánchez Fonseca, C., Yong Benitez, R., Armijo Albán, K., Guerrero Chúez, N., & Mancera-Rodríguez, N. J. (2019b). Diversidad de macroinvertebrados acuáticos en quebradas con diferente cobertura ribereña en del bosque Protector Murocomba, cantón Valencia, Ecuador. Revista de Biología Tropical, 67(4), 861–878. https://doi.org/10.15517/rbt.v67i4.35190
Zapata, D., Oleas, N. H., Páez-Vacas, M., & Tobes, I. (2021). Water Quality Assessment of the Cutuchi River Basin (Ecuador): A Review of Technical Documents. IOP Conference Series: Earth and Environmental Science, 690(1), 12058. https://doi.org/10.1088/1755-1315/690/1/012058
Published
2022-11-24
How to Cite
Urdánigo , J., Pérez, K., Intriago, E., Arriaga, G., Zambrano, S., & Prieto, O. (2022). Bioindicators of water quality with different riparian cover in the Guapara micro watershed, Ecuador. Revista De La Facultad De Agronomía De La Universidad Del Zulia, 39(4), e223952. Retrieved from https://www.produccioncientificaluz.org/index.php/agronomia/article/view/39165
Issue
Section
Environment
Copyright (c) 2022 Juan Pablo Urdánigo Zambrano, Karla Micaela Pérez Anchundia, Elian Briohildo Intriago Giler, Gabriela Jazmín Arriaga Loor, Solange Anabeth Zambrano Ganchozo, Oscar Oswaldo Prieto Benavides
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
