Regeneration of Rubus in vitro using forchlorfenuron (CPPU)

B. Millán Mendoza


The ability of N-(2-chloro-4-pyridyl)-N-phenylurea(CPPU),a synthetic compound with cytokinin-like activity, to induce regeneration of whole plants in Rubus was assessed. Regeneration was obtained from internodal segments of three tissue culture recalcitrant red raspberry cultivars Glen Prosen (32%), Glen Moy (38%) and Glen Magna (72%) using media containing low concentrations of CPPU. The ability of CPPU to induce regeneration from the cultivars Taybeny (38%) and Loch Ness (46%) was examined and the results compared with previous work. The regeneration protocol here tested provides the first step for future genetic transformation involving these three Rubus varieties.

Palabras clave

CPPU; rubus; organogenesis; regeneration

Texto completo:


Revista de la Facultad de Agronomía / Universidad del Zulia /Venezuela/ 

Correo:  / p-ISSN: 0378-7818 / e-ISSN: 2477-9407


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