Toxicidad subaguda reproductiva del herbicida Clodinafop Propargyl en conejos domésticos machos

• Samia Rebahi Mohamed Cherif Messadia University of Souk-Ahras, Laboratory of Science and Techniques for Living. B.P, Route de Annaba, Algeria. https://orcid.org/0009-0002-8074-8117
• Abdelouaheb Alouani Mohamed Cherif Messadia University of Souk-Ahras, Faculty of Sciences and Life. B.P, Route de Annaba, Algeria. https://orcid.org/0000-0001-6198-6684
• Mohamed Nadir Meguini Mohamed Cherif Messadia University of Souk-Ahras, Institute of Veterinary and Agronomic Sciences, Department of Veterinary Sciences. B.P, Route de Annaba, Algeria. https://orcid.org/0000-0002-7274-5840
• Tarek Khenenou Mohamed Cherif Messadia University of Souk-Ahras, Institute of Veterinary and Agronomic Sciences, Department of Veterinary Sciences. B.P, Route de Annaba, Algeria. https://orcid.org/0009-0000-0821-6779
• Hafiza Aidoudi Mohamed Cherif Messadia University of Souk-Ahras, Laboratory of Science and Techniques for Living. B.P, Route de Annaba, Algeria. https://orcid.org/0000-0003-2712-126X
• Djalel Eddine Rahmoun Mohamed Cherif Messadia University of Souk-Ahras, Institute of Veterinary and Agronomic Sciences, Department of Veterinary Sciences. B.P, Route de Annaba, Algeria. https://orcid.org/0000-0001-6723-4491

DOI: https://doi.org/10.52973/rcfcv-e362841

Palabras clave: Toxicidad reproductiva, Clodinafop-propargyl, morfometría, histopatología, conejos, reproducción, fibrosis, exposición a herbicida

Resumen

Varios estudios han evaluado la toxicidad reproductiva de los herbicidas. Sin embargo, los datos sobre los efectos reproductivos del clodinafop-propargil siguen siendo limitados. Por lo tanto, el presente estudio tiene como objetivo evaluar la toxicidad reproductiva del clodinafop-propargil mediante la evaluación de la toxicidad testicular subaguda en conejos machos adultos, utilizando parámetros morfométricos, histológicos y hormonales. Los animales, de 5 a 6 meses de edad, fueron asignados aleatoriamente a cuatro grupos experimentales. El grupo control mostró una ganancia de peso normal durante los 30 días del experimento, mientras que los grupos tratados presentaron retraso en el crecimiento dependiente de la dosis, especialmente en la dosis más alta. Los niveles séricos de hormonas, determinados mediante el ensayo de inmunoadsorción ligado a enzima, mostraron una disminución significativa y dependiente de la dosis en la concentración de testosterona: 11,14 ± 0,93 nmol·L–1 en el grupo control; 7,66 ± 0,67 nmol·L–1 en G1; 5,75 ± 0,81 nmol·L–1 en G2; y 0,95 ± 0,72 nmol·L–1 en G3 (P<0,05). El peso testicular también se redujo de manera significativa: 9,97 ± 0,88 g (control), 9,10 ± 4,73 g (G1), 7,83 ± 0,61 g (G2) y 1,03 ± 0,71 g (G3) (P<0,05). El examen histopatológico de testículos y epidídimos reveló lesiones graves y dependientes de la dosis en los grupos G2 y G3, incluyendo atrofia de los túbulos seminíferos, desorganización celular y vacuolización, sin alteraciones en los animales del grupo control. Estos resultados demuestran que el Clodinafop Propargyl induce una toxicidad reproductiva severa en conejos machos, afectando de forma significativa la estructura y función testicular, especialmente a dosis elevadas.

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