Asociación de polimorfismos del gen Leptina con calidad seminal en toros raza Carora

  • Belkys J. Vásquez-Marín Instituto Nacional de Investigaciones Agrícolas, Laboratorio de Biotecnología Agrícola, Maracay, Aragua, Venezuela.
  • Saúl Salazar-Sequea Instituto Nacional de Investigaciones Agrícolas, Laboratorio de Reproducción Animal. Maracay, Aragua, Venezuela.
  • Oscar De La Rosa Instituto Nacional de Investigaciones Agrícolas (INIA), Centro Nacional de Investigaciones Agrícolas (CENIAP). Maracay, Aragua, Venezuela https://orcid.org/0000-0002-9550-2366
  • Omar Verde Universidad Central de Venezuela, Facultad de Ciencias Veterinarias, Cátedra de Genética. Maracay, Aragua, Venezuela.
  • Alexis F. Marques-Urdaneta Instituto Nacional de Investigaciones Agrícolas, Laboratorio de Biotecnología Agrícola, Maracay, Aragua, Venezuela.
  • Lourdes Tibisay Vilanova-Fernández Universidad Centroccidental Lisandro Alvarado, Facultad de Ciencias Veterinarias, Cátedra de Reproducción Animal. Barquisimeto, Lara, Venezuela.
Palabras clave: Alelos, marcadores moleculares, ADN, variación genética, semen

Resumen

La presencia de toros con características seminales deficientes ocasiona fallas considerables en la eficiencia reproductiva de las unidades de producción, debido a la disminución del número de vacas preñadas, siendo más marcado en sistemas bajo programas de inseminación artificial (IA). Los rasgos de calidad seminal (CS) pueden constituir un criterio importante para la selección de machos reproductores utilizados en IA. La identificación de marcadores moleculares asociados con CS en el toro, podría facilitar la selección para estos rasgos. Este estudio tuvo como objetivo evaluar la asociación de los polimorfismos del gen Leptina, sobre la CS de toros de la raza Carora. Se evaluaron las variables volumen de eyaculado (VE), motilidad masal (MM), motilidad individual (MI) y concentración espermática (CE) de 43 toros reproductores Carora, organizados en 8 grupos de edad. Los polimorfismos nucleotídicos simples (SNP) del gen Leptina evaluados fueron: rs29004487 (SNP1), rs29004488 (SNP2), rs29004501 (SNP3) y rs29004508 (SNP4). Se utilizó un análisis de varianza mediante un modelo lineal generalizado (GLM). El factor genotipo contó con 10 niveles y el factor edad, 8 niveles. No se observó efecto del SNP1 sobre ninguna de las variables evaluadas, mientras que el SNP3 tuvo un efecto significativo sobre la CE. Los SNP2 y SNP4 presentaron un efecto altamente significativo sobre la MI y CE. Finalmente, las variables VE y MM no fueron afectadas por ninguno de los SNP estudiados. Los resultados del presente estudio sugieren marcadores potenciales para la valoración y selección genética de bovinos reproductores.

Descargas

La descarga de datos todavía no está disponible.

Citas

ALASHAWKANY, A.; SHAHROUDI, F.; NASSIRY, M.; MOUSSAVI, A.; HEYDARPOUR, M.; SADEGHI, B. Association of SNP in the exon II of leptin gene with milk and reproduction traits in Holstein Iranian cows. Biotechnol. 7 (2): 347-350. 2008.

AL-SHUHAIB, M.B. A comprehensive in silico prediction of the most deleterious missense variants in the bovine LEP gene. Bio Tech. 100(4):429-439. 2019. En línea: https://doi. org/gtqh. 08-07-2021

AMANN, R.P.; DEJARNETTE, J.M. Impact of genomic selection of AI sires on their likely utilization and methods to estimate fertility: a paradigm shift. Theriogenol. 77(5): 795-817. 2012.

AMARAL, A.; CASTILLO, J.; ESTANYOL, J.M.; BALLESCA, J.L.; RAMALHO-SANTOS, J.; OLIVA, R. Human sperm tail proteome suggests new endogenous metabolic pathways. Mol. Cell Proteomics. 12(2): 330-342. 2013.

AQUILA, S.; GENTILE, M.; MIDDEA, E.; CATALANO, S.; MORELLI, C.; PEZZI, V.; ANDO, S. Leptin secretion by human ejaculated spermatozoa. J. Clin. Endocrinol. Metab. 90: 4753-4761. 2005.

AQUILA, S.; RAGO, V.; GUIDO, C.; ZUPO, S.; CASABURI, I.; CARPINO, A. Leptin and leptin receptor in pig spermatozoa: evidence of their involvement in sperm capacitation and survival. Reprod. 136(1): 23-32. 2008.

ASOCRICA. Raza Carora. En línea: https://www.razacarora. com.ve/. 20/08/20.

BAKHTIAR, R.; ABDOLMOHAMMADI, A.; HAJARIAN, H.; NOKOUSEFAT, Z.; KALANTAR-NEYESTANAKI, D. Identification of g.170G>A and g.332G>A mutations in exon 3 of leptin gene (Bcnl and Cail) and their association with semen quality and testicular dimensions in Sanjabi rams. Anim. Reprod. Sci. 179: 49-56. 2017.

BARTH, A.D.; BRITO, L.F.; KASTELIC, J.P. The effect of nutrition on sexual development of bulls. Theriogenol. 70: 485-494. 2008.

BIŽIENĖ, R.; MORKŪNIENĖ, K.; MIŠEIKIENĖ, R.; PEČIULAITIENĖ, N.; MAKŠTUTIENĖ, N.; ŠLYŽIUS, E. Effect of single nucleotide polymorphism markers on the carcass and fattening traits in different pig populations. J. Anim. Feed Sci. 27(3): 255-272. 2018.

BUCHANAN, F.; FITZSIMMONS, C.J.; VAN KESSEL, A.G.; THUE, T.D.; WINKELMA-SIM, D.C.; SCHMUTZ, S.M. Association of a missense mutation in the bovine leptin gene with carcass fat content and leptin mRNA levels. Genet. Selec. Evolut. 34:105-16. 2002.

CHEN, M.; MANLEY, J.L. Mechanisms of alternative splicing regulation: insights from molecular and genomics approaches. Nat. Rev. Mol. Cell Biol. 10:741-54. 2009

DEB, R.; KUMAR, S.; SINGH, U.; TYAGI, S.; MANDAL, D.K.; SENGAR, G.; SINGH, R.; KUMAR, M.; SHARMA, A. Evaluation of three bovine Y specific microsatellite loci as an alternative biomarkers for semen quality traits in crossbred bull. Anim. Reprod. Sci. 142(2): 121-125. 2013.

DEB, R.; SINGH, U.; RAJA, T.V.; KUMAR, S.; TYAGI, S.; ALYETHODI, R.R.; ALEX, R.; SENGER, G.; SHARMA, S. Designing of an artificial neural network model to evaluate the association of three combined Y specific microsatellite loci on the actual and predicted post thaw motility in crossbred bull semen. Theriogenol. 83(9): 1445-1450. 2015.

DERNER, J.D.; HUNT, L.; EUCLIDES FILHO, K.; RITTEN, J.; CAPPER, J.; HAN, G. Livestock production Systems. In: Rangeland Systems. Processes, management and challenges. Briske, D. (Ed.) Springer: Cham, Suiza. Pp. 367. 2017.

DHANOA, J.K.; MUKHOPADHYAYY, C.S.; ARORA, J.S.; KAUR, S. Expression profile of Leptin and Aromatase-P450 genes and association with sperm motility in fresh bovine ejaculates. Proc. Natl. Acad. Sci. India. 86: 611-616. 2016.

DILBAR, G.H.; QURESHI, Z.I.; BABAR, M.E.; JAMIL, H.; JAVED, M.T. R159Q polymorphism in Leptin gene and its correlation with semen quality parameters in Nili-Ravi buffalo bulls. Pak. Vet. J. 39(1): 61-65. 2019.

ENGELKEN, T.; TREJO, C.; VOSS, K. Reproductive health programs for beef herds: analysis of records for assessment of reproductive performance. In: Youngquist, R. S; Threlfall, W. R. (Eds.). Current therapy in large animal theriogenology. 2nd Ed. Saunders, Philadelphia. USA. Pp. 490-496. 2007.

FAIR, S.; LONERGAN, P. Review: understanding the causes of variation in reproductive wastage among bulls. Anim. 12(s1): 53-62. 2018.

FOOD AND AGRICULTURE ORGANIZATION OF THE UNITED NATIONS (FAO). How to feed the world: Global Agriculture Towards 2050. 2009: Roma. En línea: https://bit. ly/3BzRiyx. 14/08/20.

FOOTE, A.P.; TAIT JR, R.G.; KEISLER, D.H.; HALES, K.E.; FREETLY, H.C. Leptin concentrations in finishing beef steers and heifers and their association with dry matter intake, average daily gain, feed efficiency and body composition. Domest. Anim. Endocrinol. 55: 136-141. 2016.

FOOTE, R.H. Factors influencing the quantity and quality of semen harvested from bulls, rams, boars and stallions. J. Anim. Sci. 47: 1-11. 2014.

FORTES, M.R.; REVERTER, A.; HAWKEN, R.J.; BOLORMAA, S.; LEHNERT, S.A. Candidate genes associated with testicular development sperm quality and hormone levels of inhibin, luteinizing hormone and insulin-like growth factor 1 in Brahman bulls. Biol. Reprod. 87: 58. 2012. En línea: https://doi.org/gtqg. 12/08/20.

FORTES, M.R.; REVERTER, A.; KELLY, M.; MCCULLOCH, R.; LEHNERT, S.A. Genome-wide association study for inhibin, luteinizing hormone, insulin-like growth factor 1, testicular size and semen traits in bovine species. Androl. 1: 644-650. 2013.

HERING, D.M.; OLENSKI, K.; KAMINSKI, S. Genome-wide association study for poor sperm motility in Holstein-Friesian bulls. Anim. Reprod. Sci. 146: 89-97. 2014a.

HERING, D.M.; OLENSKI, K.; RU, S.A.; KAMINSKI, S. Genome wide association study for semen volume and total number of sperm in Holstein-Friesian bulls. Anim. Reprod. Sci. 151: 126-130. 2014b.

HODŽIĆ, A.; RISTANOVIĆ, M.; ZORN, B.; TULIĆ, C.; MAVER, A.; NOVAKOVIĆ, I.; PLASESKA-KARANFILSKA, D.; PETERLIN, B. Genetic variation in leptin and leptin receptor genes as a risk factor for idiopathic male infertility. Androl. 5(1): 70-74. 2016.

HUNT, S.; McLAREN, W.; GIL, L.; THORMANN, A.; SCHUILENBURG, H.; SHEPPARD, D.; PARTON, A.; ARMEAN, I.; TREVANION, S.; FLICEK, P.; CUNNINGHAM, F. Ensembl variation resources. Database. 18: 1-12. 2018. En línea: https://doi.org/gtqj

JECMINKOVA, K.; MÜLLER, U.; KYSELOVA, J.; SZTANKOOVA, Z.; ZAVADILOVA, L.; STIPKOVA, L.; MAJZLIK, I. Association of leptin, toll-like receptor 4 and chemokine receptor of interleukin 8 C-X-C motif single nucleotide polymorphisms with fertility traits in czech Fleckvieh cattle. Asian-Australas. J. Anim. Sci. 31(11): 1721-1728. 2018. En línea: https://doi.org/gdbgkp

JONAS, E.; MARTIN, G.B.; CELI, P.; SOATTIN, M.; THOMSON, P.C.; RAADSMA, H.W. Association of polymorphisms in leptin and leptin receptor genes with circulating leptin concentrations, production and efficiency traits in sheep. Small Rumin. Res. 136: 78-86. 2016. En línea: https://doi.org/f8ndw2

KANCHAN, K.; AMAN, K.; VISHAL, S.; ANUPAMA, K.; TRILOK, N. Leptin gene transcripts study in vitro capacitated spermatozoa of buffalo bull semen. Haryana. Vet. 53(1): 50-54. 2014.

KHAKI, A.; BATAVANI, R.A.; NAJAFI, G. The in vitro effect of leptin on semen quality of water buffalo (Bubalus bubalis) bulls. Vet. Res. Forum. 41(1): 7-12. 2013.

KHOSROPOUR, S.; HAMIDI, M.; FATTAHI, A.; KHODADATI, I.; KARAMI, M.; FAZILATI, M. Leptin and leptin-receptor polymorphisms in fertile and infertile men. Syst. Biol. Reprod. Med. 63(1): 7-14. 2016.

KMIEĆ, M.; KULIG, H.; KONIK, A. Preliminary reproduction performance traits of boars results on associations between leptin gene (LEP) and some reproduction performance traits of boars. Arch. Tierz. 46: 63-70. 2003.

KOMISAREK, J.; ANTKOWIAK, I. The relationship between leptin gene polymorphisms and reproductive traits in Jersey cows. Pol. J. Vet. Sci. 10 (4): 193-197. 2007.

KOMISAREK, J. Impact of LEP and LEPR gene polymorphisms on functional traits in Polish Holstein-Friesian cattle. Anim. Sci. Pap. Rep. 28:133-141. 2010.

KUL, A.; BALTACI, A.K.; MOGULKOC, R. Effect of testosterone supplementation on leptin release in rats after castration and/ or unilateral surrenalectomy. Endokrynol. Pol. 63(2): 119-124. 2012.

LAMPIAO, F.; DU PLESSIS, S.S. Insulin and leptin enhance human sperm motility, acrosome reaction and nitric oxide production. Asian J. Androl. 10(5): 799-807. 2008.

LIU, J.; SUN, Y.; YANG, C.; ZHANG, Y.; JIANG, Q.; HUANG, J.; JU, Z.; WANG, X.; ZHONG, J.; WANG, C. Functional SNPs of INCENP affect semen quality by alternative splicing mode and binding affinity with the target BTA-miR-378 in chinese Holstein bulls. PloS One. 11(9): e0162730. 2016.

LIU, X.; JU, Z.; WANG, L.; ZHANG, Y.; HUANG, J.; LI, Q.; LI, J.; ZHONG, J.; AN, L.; WANG, C. Six novel single nucleotide polymorphisms in SPAG11 gene and their association with sperm quality traits in Chinese Holstein bulls. Anim. Reprod. Sci. 129: 14-21. 2011.

LONE, S.A.; PARAY, A.R.; MIR, S.H.; GANAIE, B.A.; SINHA, R.; SINGH, P. Breeding soundness evaluation in bulls: A review. Biomed. J. Sci. Tech. Res. 1(5): 1-4. 2017.

MANDAL, D.K.; KUMAR, M.; TYAGI, S. Effect of age on spermiogram of Holstein Friesian x Sahiwal crossbred bulls. Anim. 4(4): 595-603. 2009.

MAŃKOWSKA, A.; BRYM, P.; PAUKSZTO, Ł.; JASTRZĘBSKI, J.P.; FRASER, L. Gene polymorphisms in boar spermatozoa and their associations with post-thaw semen quality. Int. J. Mol. Sci. 21(5): 1-30. 2020.

MARTÍNEZ, R.; ROCHA, J.F.; BEJARANO, D.; GÓMEZ, Y.; ABUABARA, Y.; GALLEGO, J. Identification of SNPs in growth related genes in colombian creole cattle. Genet. Mol. Res. 15(3): 1-16. 2016.

MARTINS, F.F.; AQUILA, M.B.; MANDARIM-DE-LACERDA, C.A. Impaired steroidogenesis in the testis of leptin-deficient mice (ob/ob-/-). Acta Histochem. 119(5): 508-515. 2017.

MENEGASSI, S.R.; PEREIRA, G.R.; AGUIAR, P.R.; PEREIRA, K.S.; KOETZ JUNIOR, C.; BRACCINI NETO, J.; PERIPOLLI, V.; BERLITZ, C.G.; BARCELLOS, J.O. Candidate genes related to reproductive traits of Hereford and Braford bulls. Sémin. Cien. Agrar. 39(3): 1335-1350. 2018.

MINITAB. Users Guide. 2010. En línea: www.minitab.com. 10/01/20.

MOTA, L.F.; BONAFÉ, C.M.; ALEXANDRE, P.A.; SANTANA, M.H.; NOVAIS, F.J.; TORIYAMA, E.; PIRES, A.V.; SILVA, S.; LEME, P.R.; FERRAZ, J.B.; FUKUMASUS, H. Circulating leptin and its muscle gene expression in Nellore cattle with divergent feed efficiency. J. Anim. Sci. Biotechnol. 8(71): 1-5 2017.

MURPHY, E.M.; KELLY, A.K.; O´MEARA, C.; EIVERS, B.; LONERGAN, P.; FAIR, S. Influence of bull age, ejaculate number, and season of collection on semen production and sperm motility parameters in Holstein Friesian bulls in a commercial artificial insemination center. J. Anim. Sci. 96(6): 2408-2418. 2018.

NIKBAKHT, G.; ALI MEHR, M.R.; BAGHBANZADEH, A.; TAJIK, P.; TAMANINI, C.; EMAM, M. Leptin receptor mRNA in bull ejaculated spermatozoa. Reprod. Domestic. Anim. 45: 237-242. 2010.

NIKBIN, S.; PANANDAM, J.M.; YAAKUB, H.; MURUGAIYAH, M.; SAZILI, A.Q. Novel SNPs in heat shock protein 70 gene and their association with sperm quality traits of Boer goats and Boer crosses. Anim. Reprod. Sci. 146(3-4): 176-181. 2014.

NOGUEIRA, É.; RODRIGUES, W.B.; SILVA, C.S.; COSTA E SILVA, V.; POTIENS, J.R.; SILVA, J.C.; SUTOVSKY, P. Identification of seminal parameters predictive of conception rates in Angus and Nelore bulls used in TAI. Proceedings of the International Bull Fertility Conference. Westport. 27-30/05. Irlanda. Pp. 324. 2018.

NOWROOZI-ASL, A.; AARABI, N.; ROWSHAN-GHASRODASHTI, A. Ghrelin and its correlation with leptin, energy related metabolites and thyroidal hormones in dairy cows in transitional period. Pol. J. Vet. Sci. 19(1): 197-204. 2016.

PEGOLO, S.; CECCHINATO, A.; MELE, M.; CONTE, G.; SCHIAVON, S.; BITTANTE, G. Effect of candidate gene polymorphisms on the detailed fatty acids profile determined by gas chromatography in bovine milk. J. Dairy Sci. 99: 4558-4573. 2016.

PERUCATTI, A.; DIMEO, G.P.; VALLINOTO, M.; KIERSTEIN, G.; SCHNEIDER, M.P.; INCARNATO, D.; CAPUTI, A.; MOHAMMADI, G.; VONGHIA, G.; SILVA, G.; BRENIG, B.; IANUZZI, L. FISH-mapping of LEP and SLC26A2 genes in sheep, goat and cattle R-banded chromosomes: comparison between bovine, ovine and caprine chromosome 4(BTA4/OAR4/ CHI4) and human chromosome 7 (HSA7). Cytogenet. Genome Res. 115: 7-9. 2006.

PERUMAL, P. Scrotal circumference and its relationship with testicular growth, age and body weight in Tho Tho (Bos indicus) bulls. Int. Sch. Res. Notices. 2014: 1-6. 2014.

PORKKA, M.; GERTEN, D.; SCHAPHOFF, S.; SIEBERT, S.; KUMMU, M. Causes and trends of water scarcity in food production. Environ. Res. Lett. 11(1): 1-13. 2016.

RAMÍREZ-BELLO, J.; VARGAS-ALARCÓN, G.; TOVILLAZÁRATE, C.; FRAGOSO, J.M. Polimorfismos de un solo nucleótido (SNP): implicaciones funcionales de los SNP reguladores (rSNP) y de los SNP-ARN estructurales (srSNP) en enfermedades complejas. Gac. Med. Mex. 149: 220-228. 2013.

RODRÍGUEZ-MARTÍNEZ, H. Semen evaluation technique and their relationship with fertility. Anim. Reprod. 10(3): 148-159. 2013.

SALAZAR, S.; DE LA ROSA, O.; MARQUES, A.; VILANOVA, L.; VÁSQUEZ, B. Caracterización de polimorfismos del gen Leptina en sementales de la raza Carora. Rev. Científ. FCV-LUZ. XXX(2): 32-43. 2020.

SENGUPTA, P.; BHATTACHARYA, K.; DUTTA, S. Leptin and male reproduction. Asian Pac. J. Reprod. 8(5):220-226. 2019.

SHOJAEI, H.A.; CLAUDE, R. Modern reproductive technologies and breed improvement. In: The genetics of cattle, Garrick, D; Ruvinsky, A. (Eds.). Iowa State University, Iowa, Estados Unidos. Pp. 284-317. 2015.

SILVA, D.B.; CRISPIM, B.A.; SILVA, L.E.; OLIVEIRA, J.A.; SIQUEIRA, F.; SENO, L.O.; GRISOLIA, A.B. Genetics variations in the leptin gene associated with growth and carcass traits in Nellore cattle. Genet. Mol. Res. 13(2): 3002-3012. 2014.

SNOJ, T.; KOBAL, S.; MAJDIC, G. Effects of season, age and breed on semen characteristics in different Bos taurus breeds in 31-year retrospective study. Theriogenol. 79:847-852. 2013.

THUNDATHIL, J.C.; DANCE, A.L.; KASTELIC, J.P. Fertility management of bulls to improve beef cattle productivity. Theriogenol. 86(1):397-405. 2016.

TIAN, J.; ZHAO, Z.; ZHANG, L.; ZHANG, Q.; YU, Z.; LI, J.; YANG, R. Association of the leptin gene E2-169T>C and E3-299T>A mutations with carcass and meat quality traits of the chinese Simmental-cross steers. Gene. 518: 443-448. 2013.

TRAKOVICKÁ, A.; MORAVČIKOVÁ, N.; KASARDA, R. Genetic polymorphisms of leptin and leptin receptor genes in relation with production and reproduction traits in cattle. Acta Biochim. Pol. 60 (4): 783-787. 2013

VALOUR, D.; MICHOT, P.; EOZENOU, C.; LEFEBVRE, R.; BONNET, A.; CAPITAN, S.; UZBEKOVA, E.; SELLEM, E.; PONSART, C.; SCHIBLER, L. Dairy cattle reproduction is a tightly regulated genetic process: Highlights on genes, pathways, and biological processes. Anim. Front. 5(1): 32-41. 2015.

WANG, C.; ZHANG, H.; NIU, L.; GUO, J.; JIA, X.; WANG, L.; LI, L.; ZHANG, H.; ZHONG, T. The novel SNPs of leptin gene and their associations with growth traits in chinese Nanjiang yellow coat. Gene. 572(1): 35-41. 2015.

WANG, X.; ZHANG, X.; HU, L.; LI, H. Exogenous leptin affects sperm parameters and impairs blood testis barrier integrity in adult male mice. Reprod. Biol. Endocrinol. 55(16): 1-11. 2018.

WATHES, D.C.; DISKIN, M.G., Reproduction, events and management: Mating management: Fertility. Reference Module in Food Science. 1-11 2016. En línea: https://doi.org/gnnk. 11/07/20.

YE, S.; DHILLON, S.; KE, X.; COLLINS, A.R.; DAY, I.N. An efficient procedure for genotyping single nucleotide polymorphisms. Nucleic Acids Res. 29(17): e88. 2001

Publicado
2021-09-22
Cómo citar
1.
Vásquez-Marín BJ, Salazar-Sequea S, De La Rosa O, Verde O, Marques-Urdaneta AF, Vilanova-Fernández LT. Asociación de polimorfismos del gen Leptina con calidad seminal en toros raza Carora. Rev. Cient. FCV-LUZ [Internet]. 22 de septiembre de 2021 [citado 29 de marzo de 2024];31(4):147-56. Disponible en: https://www.produccioncientificaluz.org/index.php/cientifica/article/view/36725
Sección
Producción Animal