Ruminal fistulation and cannulation of crossbred cows using the one-stage technic and plastisol ruminal cannulas

• Carlos David Moreno-Manresa Universidad Nacional Experimental del Táchira (UNET), Departamento de Ingeniería de Producción Animal. San Cristóbal, Táchira, Venezuela
• Néstor Enrique Hernández-Rodríguez Universidad Nacional Experimental del Táchira (UNET), Departamento de Ingeniería de Producción Animal. San Cristóbal, Táchira, Venezuela
• Robert Emilio Mora-Luna Universidad Nacional Experimental del Táchira (UNET), Decanato de Investigación, Coordinación de Investigación Agropecuaria. San Cristóbal, Táchira, Venezuela - Universidade Federal do Norte do Tocantins (UFNT), Posdoctorante en el Programa de Postgrado en Ciencia Animal Tropical. Araguaína, Tocantins, Brasil https://orcid.org/0000-0001-8439-784X
• Ana María Herrera-Angulo Universidad Nacional Experimental del Táchira (UNET), Decanato de Investigación, Coordinación de Investigación Agropecuaria. San Cristóbal, Táchira, Venezuela
• Pablo Herrera Universidad Nacional Experimental Simón Rodríguez (UNESR), Estación Experimental “La Iguana”. Santa María de Ipire, Guárico, Venezuela
• Norelys Rodríguez-Romero Universidad Nacional Experimental del Táchira (UNET), Decanato de Investigación, Coordinación de Investigación Agropecuaria. San Cristóbal, Táchira, Venezuela

DOI: https://doi.org/10.52973/rcfcv-e32104

Keywords: Cattle, ruminal cannula, surgery

Abstract

The aim of this study was to describe the process of ruminal fistulation and cannulation (RFC) using the one-stage technique and plastisol cannulas (PC) in crossbred cows (from ⅜Holstein × ⅝Zebu up to ⅝Holstein × ⅜Zebu), as well as to describe the post-operative period. Seven crossbred cows averaging 386 ± 32 kilograms of body weight were used. Prior to surgery, two animals were fasted for 24 hours (h) and five animals were fasted for 48 h. All surgeries were performed with animals restrained in the chute station. Approximately 18.8 centimeter incision in the center of the left paralumbar fossa was done. The skin and muscular planes incision in all animals was made from dorsal to ventral direction, while the rumenotomy was made from dorsal to ventral direction in five animals, and from cranial to caudal direction with a 45° angle in two animals. The average surgery time was 70 minutes. The 48-h fast decreased ruminal content, which reduced its flow over the wound at the time of surgery, compared to animals 24-h fasted. During the postoperative period, the use of intramuscular oxytetracycline was preferred, and wound cleaning was performed every 48 h. The restraint sutures were removed after 10 days. Modifying the orientation and angle of the rumenotomy incision (cranio-caudal) accelerated healing and facilitated the postoperative period. The healing time varied between 4 to 7 weeks depending on the direction of the ruminotomy and type of antibiotic. In crossbred cows, the RFC technic of one-stage and PC placement, is possible to perform safely and quickly with basic facilities to restrain the animal, and during the postoperative period, wound cleaning can be performed every 48 h.

References

AZIZI, S.; PIR-MOHAMMADI, R.; POUR-HASANI, F. A two stage rumen cannulation technique in sheep. J. Anim. Vet. Adv. 6: 29-32. 2007.

BALCH, C. C.; COWIE, A. T. Permanent rumen fistulae in cattle. Cornell Vet. 52: 206-214. 1962.

BALCH, C. C.; JOHNSON, V. W. A pneumatic cannula and bung for rumen fistulae in cattle. Vet. Rec. 60: 446. 1948.

BOTERO, R. Fistulación de bovinos a rumen cerrado. Past. Trop. 10: 38-40. 1988.

CASTILLO, C.; HERNÁNDEZ, J. Ruminal fistulation and cannulation: A necessary procedure for the advancement of biotechnological research in ruminants. Animals. 11 (7): 1-13. 2021. https://doi.org/htf7.

ELIZONDO-SALAZAR, J.A; MONGE-ROJAS, C.R. Fistulación en bovinos y uso de la técnica de degradabilidad ruminal para análisis de alimentos. Nutr. Anim. Trop. 14: 209-229. 2020. https://doi.org/htf8.

FONDO NACIONAL DE CIENCIA TECNOLOGIA E INNOVACION (FONACIT). Normas para el uso de animales en docencia e investigación. En: Código de Bioética y Bioseguridad. 2da. Ed. Fondo Nacional de Ciencia y Tecnología. Ministerio de Ciencia y Tecnología. Caracas, Venezuela. Pp 19-20. 2002.

GHAZY, A. One-stage technique for ruminal fistulation in rams. Alex. J. Vet. Sci. 52: 109-117. 2017. https://doi.org/htf9.

HOLMBAK-PETERSEN, R.; TOBIA, C.; ROSENDO, O.; DIAZ, M.; SALDIVIA, C.; CHACÓN, A.; VELÁSQUEZ, J. Experiencia con cánulas ruminales de plastisol para bovinos. Gac. Cien. Vet. 12: 67-71. 2007.

JOHNSON, R.R. Techniques and procedures for in vitro and in vivo rumen studies. J. Anim. Sci. 25: 855-875. 1966. https://doi.org/htgb.

LAFLIN, S.L.; GNAD, D.P. Rumen cannulation: procedure and use of a cannulated bovine. Vet. Clin. N. Am. Food Anim. Pract. 24: 335-340. 2008. https://doi.org/csqxv9.

LOZIER, J.W.; NIEHAUS, A.J. Surgery of the forestomach. Vet. Clin. Food Anim. 32: 617-628. 2016. https://doi.org/htgd.

MARTINEAU, R.; PROULX, J.G.; CÔRTES, C.; BRITO, A.F.; DUFFIELD, T.F. Two-stage rumen cannulation technique in dairy cows. Vet. Surg. 44: 551-556. 2015. https://doi.org/htgf.

McSWEENEY, C.S. Cannulation of the rumen in cattle and buffaloes. Aust. Vet. J. 66: 266-268. 1989. https://doi.org/bxhbpk.

MOATE, P.J.; WILLIAMS, S.R.O., DEIGHTON, M.H.; HANNAH, M.C.; RIBAUX, B.E.; MORRIS, G.L.; JACOBS, J.L.; HILL, J.; WALES, W.J. Effects of feeding wheat or corn and of rumen fistulation on milk production and methane emissions of dairy cows. Anim. Prod. Sci. 59: 891-905. 2018. https://doi.org/gh2d99.

MORA-LUNA, R.E.; CHICCO, C.F.; HERRERA-ANGULO, A.M.; GODOY, S.; GARMENDIA, J. Suplementación con fuentes de proteína degradable y no degradable en el rumen en vacas alimentadas con Urochloa humidicola. II. Fermentación ruminal, degradación de materia orgánica y química sanguínea en vacas mestizas. Rev. Científ. FCV-LUZ. XXV(1): 63-73. 2015.

MUZZI, L.A.L.; MUZZI, R.A.L.; GABELLINI, E.L.A. Técnica de fistulação e canulação do rúmen em bovinos e ovinos. Ciên. Agrotec. 33: 2059-2064. 2009. https://doi.org/crz9xb.

RAMIN, M.; HUHTANEN, P. Development of an in vitro method for determination of methane production kinetics using a fully automated in vitro gas system—A modelling approach. Anim. Feed Sci. Technol. 174: 190-200. 2012. https://doi.org/f23dq8.

ROCHA-FILHO, R.R.; SANTOS, D.C.; VÉRAS, A.S.C.; SIQUEIRA, M.C.B.; NOVAES, L.P.; MORA-LUNA, R.; MONTEIRO, C.C.F.; FERREIRA, M.A. Can spineless forage cactus be the queen of forage crops in dryland areas?. J. Arid Environ. 186: 104426. 2021. https://doi.org/htgj.

SIQUEIRA, M.; CHAGAS, J.; MONNERAT, J. P.; MONTEIRO, C.; MORA-LUNA, R.; DUBEUX JR, J.; DILORENZO, N.; RUIZ-MORENO, M.; FERREIRA, M. Nutritive value, in vitro fermentation, and methane production of cactus cladodes, sugarcane bagasse, and urea. Animals. 11: 1266. 2021. https://doi.org/gmphvq.

SIQUEIRA, M.C.B.; FERREIRA, M.A.; MONNERAT, J.P.I.S.; SILVA, J.L.; COSTA, C.T.F.; CONCEIÇÃO, M.G.; ANDRADE, R.P.X.; BARROS, L.J.A.; MELO, T.T.B. Optimizing the use of spineless cactus in the diets of cattle: Total and partial digestibility, fiber dynamics and ruminal parameters. Anim. Feed Sci. Technol. 226: 56-64. 2017. https://doi.org/htgk.

STODDAR, G.E.; ALLEN, N.N.; HALE, W.H.; POPE, A.L.; SORENSEN, D.K.; WINCHESTER, W.R.A Permanent rumen fistula cannula for cow and sheep. J. Anim. Sci. 10: 417-423. 1951. https://doi.org/htgm.