Bovine Herpesvirus-1 antibodies levels and associated Risk Factors in unvaccinated Dairy Herds from tropical wet weather, Ecuador

• Hitler Cedeño-Sánchez Universidad Laica Eloy Alfaro de Manabí, Extensión Chone, carrera de Ingeniería Agropecuaria. Chone, Manabí, Ecuador
• Brinner Burgos-García Universidad Laica Eloy Alfaro de Manabí, Extensión Chone, carrera de Ingeniería Agropecuaria. Chone, Manabí, Ecuador
• Jhoana Zambrano-Aveiga Universidad Laica Eloy Alfaro de Manabí, Extensión Chone, carrera de Ingeniería Agropecuaria. Chone, Manabí, Ecuador
• Marcia Jurado-Hidalgo Universidad Laica Eloy Alfaro de Manabí, Extensión Chone, carrera de Ingeniería Agropecuaria. Chone, Manabí, Ecuador
• Patricia Zambrano-Moreira Universidad Laica Eloy Alfaro de Manabí, Extensión Chone, carrera de Ingeniería Agropecuaria. Chone, Manabí, Ecuador
• María Lugo-Almarza Clínica Veterinaria Bulldog. Santo Domingo, Santo Domingo de los Tsáchilas, Ecuador
• María Gabriela Farías Universidad Laica Eloy Alfaro de Manabí, Extensión Chone, carrera de Ingeniería Agropecuaria. Chone, Manabí, Ecuador
• Francisco Angulo-Cubillán Universidad UTE, Sede Santo Domingo, Facultad de Ciencias de la Salud Eugenio Espejo, carrera de Medicina Veterinaria, Veterinary Science Research Group. Santo Domingo, Santo Domingo de los Tsáchilas, Ecuador - Universidad del Zulia, Facultad de Ciencias Veterinarias, Departamento de Sanidad Animal. Maracaibo, Zulia, Venezuela

DOI: https://doi.org/10.52973/rcfcv-e32088

Keywords: Dairy herds, BHV-1, seroprevalence, risk factors, unvaccinated

Abstract

In order to determine the antibodies levels against Bovine Herpesvirus type 1 (BHV-1) and associated risk factors in unvaccinated dairy herds of tropical wet weather of Ecuador, an investigation was carried out in thirteen production units (PU), in the Chone Canton, Province of Manabí. The sample size was calculate by cluster sampling and the variables to be evaluated were antibodies levels frequency, age, sex, ocular or vulvovaginal lesions, breed and PU. One hundred eighty three blood samples were taken from the jugular or caudal vein and labeled for transfer to the laboratory. The tubes were centrifuged at 3,220 g for 15 minutes and the sera were transferred to Eppendorf tubes that once labeled were stored at –20°C until processing. Antibodies levels against HBV-1 were measured through of competitive ELISA technique, and calculated with simples statistical. The risk factors were estimated through the Odds ratio and relative risk, and the significance level through the Chi-square. The general antibodies levels frequency was 58.47 %. According age, 28.57 % in animals under two years old (yr.old) and 67.38 % for cattle over two yr.old. According to the sex, males showed 18.18 % and females 82.61 %. The determined risk factors (P<0.05) were age and sex, with a significant relative risk (P<0.05) of 3.31 and 2.32, respectively. The unvaccinated dairy herds of Western lowlands of Ecuador, have high antibodies levels frequency against BHV-1, with females over two yr.old at the highest risk of presenting infectious bovine rhinotracheitis (IBR).

References

ACKERMANN, M.; ENGELS, M. Pro and contra IBR-eradication. Vet. Microbiol. 113 (3-4): 293-302. 2006.

AL-HAMMADI, M.; HEMIDA, M. Sero-prevalence of common bovine respiratory viral diseases in Saudi Arabia. Assiut Vet. Med. J. 60: 76-81. 2014.

BETANCUR-HURTADO, C.; CASTAÑEDA-TERNERA, J.; GONZÁLEZ-TOUS, M. Immunopathology of bovine respiratory complex in calves in the Municipality of Monteria, Colombia. Rev. Científ. FCV-LUZ. XXVII (2): 95–102. 2017.

BLICKENSTORFER, S.; ENGELS, M.; GUERDAT, C.; SAUCY, C.; REIST, M.; SCHWERMER, H.; PERLER, L. Rhino-trachéite infectieuse bovine (IBR) dans le canton du Jura: enquête épidémiologique. Schweiz. Arch. Tierheilk. 152 (12): 555-560. 2010.

CALLABY, R.; TOYE, P.; JENNINGS, A.; THUMBI, S.; COETZER, J.; VAN WYK, I.; HANOTTE, O.; MBOLE-KARIUKI, M.; BRONSVOORT, B.; KRUUK, L.; WHOOLHOUSE, M.; KIARA, H. Seroprevalence of respiratory viral pathogens in indigenous calves in Western Kenya. Res. Vet. Sci. 108: 120-124. 2016.

CAMACHO, R.; CASTELLANOS-DOMINGUEZ, Y.; DÍAZ, W.; VÁSQUEZ, M. Presence of IgG antibodies against reproductive infections in breeding bulls of Magdalena Medio, Colombia. Rev. Colomb. Cien. Pec. 28: 323-330. 2015.

CARBONERO, A.; SAA, L.; JARA, D.; GARCÍA-BOCANEGRA, I.; ARENAS, A.; BORGE, C.; PEREA, A. Seroprevalence and risk factors associated to Bovine Herpesvirus-1 (BHV-1) infection in non-vaccinated dairy and dual purpose cattle herds in Ecuador. Prev. Vet. Med. 100(1): 84-88. 2011.

CÓRDOVA-IZQUIERDO, A.; CÓRDOVA-JIMÉNEZ, C.; CÓRDOVA-JIMÉNEZ, M.; SALTIJERAL-OAXACA, J.; RUIZ-LANG, C.; XOLALPA-CAMPOS, V.; CORTÉS-SUÁREZ, S.; GUERRA-LIERA, J. Seroprevalencia de enfermedades causantes de aborto bovino en el trópico húmedo mexicano. Rev. Vet. 18(2): 139–142. 2007.

FERREIRA L.; PAJEÚ, B.; MELO, J.; BALTAZAR, J.; ALVES, A.; BEZERRA, B.; ALVES, S.; PINHEIRO, J. Occurrence of Bovine Viral Diarrhea (BVDV) and Bovine Infectious Rhinotracheitis (IBR) Virus Infections in Buffaloes in Pernambuco State – Brazil. Act. Sci. Vet. 45: 1459: 1-8. 2017.

GIANGASPERO, M.; SAVINI, G.; ORUSA, O.; OSAWA, T.; HARASAWA, R. Prevalence of antibodies against Parainfluenza virus type 3, Respiratory syncitial virus and bovine Herpesvirus type 1 in sheep from Northern Prefectures of Japan. Vet. Ital. 49(3): 285-289. 2013.

INSTITUTO NACIONAL DE METEOROLOGÍA E HIDROLOGÍA (INAMHI), Ecuador. Anuarios Meteorológicos . Nº 51-2011. Cod Estación M0005, Portoviejo-Manabí. En línea: http://www.inamhi.gob.ec/. 27-05-2021.

JUNQUEIRA, J.; FREITAS, J.; ALFIERI, A.; ALFIERI, A. Reproductive performance evaluation of a beef cattle herd naturally infected with the BHV-1, BVDV and Leptospira hardjo. Cien. Agrar. 27(3): 471-480. 2006.

KNOPF, L.; SCHWERMER, H.; STÄRK, K. A stochastic simulation model to determine the sample size of repeated national surveys to document freedom from bovine herpesvirus-1 (BHV-1) infection. BMC Vet. Res. 3:10. 2007.

LOI, I.; THIRY, J.; ATTILI, A.; PREZIUSO, S.; CUTERI, V. Control Strategies for Infectious Bovine Rhinotracheitis (IBR) in Italy. XX International Congress of Mediterranean Federation of Health and Production of Ruminants, Assiut University, Al Fath, Assiut, 19-22 February 2013. Egypt. Pp. 115-120. 2013.

LUCCHESE, L.; BENKIRANE, A.; HAKIMI, I.; EL IDRISSI, A.; NATALE, A. Seroprevalence study of the main causes of abortion in dairy cattle in Morocco. Vet. Ital. 52(1): 13-19. 2016.

MORE, S. A case for increased private sector involvement in Ireland’s national animal health services. Irish Vet. J. 61(2): 92-100. 2008.

NORWEGIAN NATIONAL RESEARCH ETHICS COMMITTEES. Ethical guidelines for the use of animals in research. Kongens gate 14, 0153 Oslo. Pp. 1. 2018.

NUOTIO, L.; NEUVONEN, E.; HYYTIÄINEN, M. Epidemiology and eradication of infectious bovine rhinotracheitis/infectious pustular vulvovaginitis (IBR/IPV) virus in Finland. Act. Vet. Scandinav. 49: 3. 2007.

OFFICE INTERNATIONAL DES EPIZZOTIES (OIE) – WORLD ORGANISATION FOR ANIMAL HEALTH (WOAH). Infectious bovine rhinotracheitis/infectious pustular vulvovaginitis. Terrestrial Manual. 2.4.13. Pp. 1139-1157. 2010.

RAAPERI, K.; BOUGEARD, S.; ALEKSEJEV, A.; ORRO, T.; VILTROP, A. Association of herd BRSV and BHV-1 seroprevalence with respiratory disease and reproductive performance in adult dairy cattle. Act. Vet. Scandinav. 54: 4. 2012.

RÍOS-UTRERA, A.; ROSETE-FERNÁNDEZ, J.; ZÁRATE-MARTÍNEZ, J.; FRAGOSO-ISLAS, A.; OLAZARÁN-JENKINS, S.; GRANADOS-ZURITA, L.; BANDA-RUIZ, V.; SOCCI-ESCATELL, G. Infectious bovine rhinotracheitis: Determination of the prevalence of antibodies in mexican nonvaccinated cows in the States of Tabasco, Puebla and Veracruz. Rev. Científ. FVC-LUZ. XXVIII(5): 349–359. 2018.

ROLA, J.; LARSKA, M.; SOCHA, W.; ROLA, J.; MATERNIAK, M.; URBAN-CHMIEL, R.; THIRY, E.; ŻMUDZIŃSKI, J. Seroprevalence of bovine herpesvirus-1 related alphaherpesvirus infections in free-living and captive cervids in Poland. Vet. Microbiol. 204: 77-83. 2017.

STATISTIC ANALYSIS SYSTEM INSTITUTE (SAS). Cary, NC 27513, USA. Version 6. 1996.

SARAVANAJAYAM, M.; KUMANAN, K.; BALASUBRAMANIAM, A. Seroepidemiology of infectious bovine rhinotracheitis infection in unvaccinated cattle. Vet. World. 8(12): 1416-1419. 2015.

SCHUNICHT, O.; BOOKER, C.; JIM, K.; GUICHON, T.; WILDMAN, B.; HILL, B. Comparison of a multivalent viral vaccine program versus a univalent viral vaccine program on animal health, feedlot performance and carcass characteristics of feedlot calves. Can. Vet. J. 44: 43–50. 2003.

TURIN, L.; RUSSO, S.; POLI, G. BHV-1: New Molecular Approaches to Control a Common and Widespread Infection. Mol. Med. 5: 261-284. 1999.

URDANETA-FERNÁNDEZ, M.; URDANETA, A.; PARRA, A.; CHACÍN, E.; RAMÍREZ-BARRIOS, R.; ANGULO-CUBILLÁN, F. Prevalencia y grado de infección de helmintos gastrointestinales en rebaños bovinos doble propósito del municipio Miranda del estado Zulia, Venezuela. Rev. Univ. Zulia. 2(2): 184–193. 2011.

VAN DRUNEN, S.; VAN DEN HURK, L.; MYERS, D.; DOIG, P.; KARVONEN, B.; HABERMEHL, M.; BABIUK, L.; JELINSKI, M.; VAN DONKERSGOED, J.; SCHLESINGER, K.; RINEHART, C. Identification of a mutant bovine herpesvirus-1 (BHV-1) in post arrival outbreaks of IBR in feedlot calves and protection with conventional vaccination. Can. J. Vet. Res. 65: 81-88. 2001.

WOODBINE, K.; MEDLEY, G.; MOORE, S.; RAMÍREZ-VILLAESCUSA, A.; MASON, S.; GREEN, L. A four-year longitudinal sero-epidemiological study of bovine herpesvirus type-1 (BHV-1) in adult cattle in 107 unvaccinated herds in South West England. BMC Vet. Res. 5: 5. 2009.

ZAPATA-OSSA, H.; CUBIDES-MUNÉVAR, A.; LÓPEZ, M.; PINZÓN-GÓMEZ, E.; FILIGRANA-VILLEGAS, P.; CASSIANI-MIRANDA, C. Cluster sampling in health surveys. Rev. Salud Púb. 13(1): 141-151. 2011.

ZHANG, J.; YU, K. What is the relative risk? J. Am. Med. Assoc. 280: 1690-1691. 1998.