Development of a method for detection and quantification of tetracyclineresidues in cheese by high-performance liquid chromatography (HPLC)
Desarrollo de un método de detección y cuantificación de residuos de tetraciclina en quesos por cromatografía liquida de alta eficiencia (HPLC)
Keywords:
C r o m a t o g r a p h y h i g h p r e s s u r e l i q u i d ( H P L C ), c h e e s e, tetracycline, anti-bacterial agents
Abstract
The aim of this study was to develop a high-precision methodology that would allow the detection and quantification of tetracycline residues in cheeses using the Liquid Chromatography High Efficiency (HPLC) analytical technique. As a result, a method was generated in which tetracycline residues were detected in complex biological matrices such as cheese. Conditions of temperature (30 ° C), wave length (265 nanometres (nm)), volume (20 microlitres (μL) and injection flow (1 millilitres mL /minutes (min)) were established, as well as the mobile phase used with methanol: acetonitrile: oxalic acid (74 : 13: 13% volume/ volume (v/v)) for the assay on the HPLC machine. Calibration curves with coefficient of determination (R2) close to 1 were obtained, with concentrations of standard solutions of 2.0 parts per million (ppm), 1.5 ppm, 1.0 ppm, 0.8 ppm, 0.6 ppm, 0.5 ppm, for tetracycline. The method presented quantification limits for tetracycline of 0.1 milligrams/Litter (mg/L) and detection limit of 0.02 mg/L, values that are below the maximum limits allowed by the Colombian standard for milk. On the other hand, conditions were established to carry out the extraction process of the antibiotic residues in complex biological matrices, such as buffer, centrifugation time and temperature, type of cartridge for purification and recovery reagent, among others. It is concluded that the recovery percentage was adequate, and it is evident that the method allows the detection of antibiotic residues from biological samples.
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References
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SENYUVA, H; OZDEN, T; SARICA, DY. High-performance liquid chromatographic determination of oxytetracycline residue in cured. Turk .J. Chem. 4: 395-400. 2000.
STOLKER, AAM; BRINKMAN, UAT. Analytical strategies for residue analysis of veterinary drugs and growth- promoting agents in food-producing animals—a review. J. Chromatogr.A. 1067: 15-53.2015.
TORRES, M. Determinación de niveles de tetraciclina y oxitetraciclina en leche cruda en la Asociación Copla (corporación productora de leche de Alóag) de la parroquia Alóag del cantón Mejía. Univ. Central del Ecuador. Tesis de Grado .82 pp. 2015.
BRICEÑO FEREIRA, E; DEL C, BRITO; ECHENIQUE, R.; DÍAZ RIVERA, H; Y, COLINA MARTINEZ; MANIGLIA MÉRIDA, G. C; & ARRIETA MENDOZA, D. 2019. Determinación de residuos de cloranfenicol y sulfamidas en leches de larga duración, en la ciudad de Maracay , Venezuela. Revista Científica De La Facultad De Ciencias Veterinarias De La Universidad Del Zulia, 28(2), 121 - 128. Recuperado a partir de https://produccioncientificaluz.org/ index.php/cientifica/article/view/29200
VIÑAS, P; BALSALOBRE, N; LÓPEZ-ERROZ, C; HERNÁNDEZ-CÓRDOBA, M. Liquid chromatography with ultraviolet absorbance detection for the analysis of tetracycline residues in honey. J .Chromatogr. A. 1022: 125-129. 2004.
WILSON, JS; OTSUKI, T; MAJUMDSAR, B; Balancing food safety and risk: do drug residue limits affect international trade in beef J .Int. Trade. Econ. Dev. 2: 37-402. 2003.
ZHOU, J; XUE, X; LI, Y; ZHANG, J; CHEN, F; WU, L; CHEN, L; ZHAO, J. Multiresidue determination of tetracycline antibiotics in propolis by using HPLC-UV detection with ultrasonic-assisted extraction and two-step solid phase extraction.Food Chem.115: 1074-1080. 2009.
AGUILERA, C; HERRERA, C; PONCE, J. Determinación de oxitetraciclina en salmonídeos Implementación, validación y aplicación de un nuevo método para la determinación de oxitetraciclina por HPLC en tejido muscular de salmonídeos. J. Aquat. Res. 38: 227-233.2010.
ANDRADE-EIROA, A; CANLE, M; LEROY-CANCELLIERI, V; CERDÀ, V; Solid-phase extraction of organic compounds: a critical review. part I. trac-Trend. Anal. Chem. 80: 641- 654. 2016.
BOULTIF, L; ZEGHILET, N; CHEBIRA, B; AGABOU, A; MEKROUD, A. Validation of a high performance liquid chromatography (HPLC) method for the determination of oxytetracycline residues in milk. Adv. Anim. Vet .Sci. 2: 574-581. 2014.
BRANDSTETEROVA, E; KUBALEC, P; BOVANOVA, L; SIMKO, P; BEDNARIKOVA, A; MACHACKOVA, L. SPE and MSPD as pre-separation techniques for HPLC of tetracyclines in meat, milk and cheese. Z. Lebensm. Unters. F A. 205: 311-315. 1997.
DENOBILE, M; NASCIMIENTO, E. Validação de método para determinação de resíduos dos antibióticos oxitetraciclina, tetraciclina, clortetraciclina e doxiciclina em leite por cromatografia líquida de alta eficiência. Rev. Bras. Cien. Farm. 40: 209-218. 2004.
DLUHOŠOVÁ, S; KANIOVÁ, L; BORKOVCOVÁ, I; VORLOVÁ, L. A study of changes in the concentrations of tetracycline antibiotics in honey. J. Food Sci. Tech .Mys. 1: 25-27. 2016.
FRANCO, J; ROMERO, M; TABORDA, G. Determinación de niveles residuales de tetraciclina en canales bovinas por la técnica de ELISA en el frigorífico Friogan (La Dorada). Biosalud. 7: 47-55. 2008.
FREITAS, A; BARBOSA, J; RAMOS, F. Multidetection of antibiotics in liver tissue by ultra-high-pressure- liquid-chromatography–tandem mass spectrometry. J. Chromatogr. B. 22: 49-54. 2015.
FURUSAWA, N. Rapid liquid chromatographic determination of oxytetracycline in milk. J. Chromatogr. A. 839: 247-251. 1999.
FURUSAWA, N. Simplified liquid-chromatographic determination of residues of tetracycline antibiotics in eggs. J .Chromatogr. 53: 47-50. 2001.
INTERNACIONAL BUSSINES MACHINES (IBM) CORP. Released 2015. IBM SPSS Statistics for Windows, Version 23.0. Armonk, NY: IBM Corp.
KUMAR, V; GUPTA, J. Synthesis of policies among stakeholders to combat antimicrobial resistance in livestock animals: Indian perspective. J. Pharmacol. Phytoch. 7: 3649-3653. 2018.
MEINERTZ, JR. Liquid chromatographic determination of oxytetracycline in edible fish fillets from six species of fish. JAOAC. Int. 81: 702-708. 1998.
MINISTERIO DE SALUD Y PROTECCIÓN SOCIAL. Resolución N.° 00001382 de 2 de mayo de 2013. En Línea : https://www.minsalud.gov.co/sites/hrid/Lists/ BibliotecaDigital/RIDE/DE/DIJ/resolucion-1382-de-2013. pdf. 22 Julio 2020.
MIRZAEI, R; YUNESIAN, M; NASSERI, S; GHOLAMI, M; JALILZADEH, E; SHOEIBI, S; BIDSHAHI, HS. An optimized SPE-LC-MS/MS method for antibiotics residue analysis in ground, surface and treated water samples by response surface methodology- central composite design. J. Environ. Health Sci. 15: 21. 2017.
NIETO, LE; GIRALDO, R. Tendencies of biotechnology, innovation and development in Colombia. Luna Azul. 41:348-364.2015.
OKA, H; ITO, Y; MATSUMOTO, H. Chromatographic analysis of tetracycline antibiotics in foods. J. Chromatogr. A. 82: 109-133.2000.
ORWA, JD; MATOFARI, JW; MULIRO, PS; LAMUKA, P. Assessment of sulphonamides and tetracyclines antibiotic residue contaminants in rural and peri urban dairy value chains in Kenya. Int. J. Food Contaminat. 4: 5. 2017.
PRADO, CK; FERREIRA, FD; BANDO, E; MACHINSKI, M. Oxytetracycline, tetracycline, chlortetracycline and doxycycline in pasteurised cow’s milk commercialised in Brazil. Food Addit. Contam .B. 8: 81-84. 2015.
RAMIREZ - LOPEZ, C. Physical properties of foods View project Physical properties of dairy products View project. 2012. Universidad de las Américas Puebla. On Line: https:// www.researchgate.net/publication/303959697. 8 Julio 2020.
SENYUVA, H; OZDEN, T; SARICA, DY. High-performance liquid chromatographic determination of oxytetracycline residue in cured. Turk .J. Chem. 4: 395-400. 2000.
STOLKER, AAM; BRINKMAN, UAT. Analytical strategies for residue analysis of veterinary drugs and growth- promoting agents in food-producing animals—a review. J. Chromatogr.A. 1067: 15-53.2015.
TORRES, M. Determinación de niveles de tetraciclina y oxitetraciclina en leche cruda en la Asociación Copla (corporación productora de leche de Alóag) de la parroquia Alóag del cantón Mejía. Univ. Central del Ecuador. Tesis de Grado .82 pp. 2015.
BRICEÑO FEREIRA, E; DEL C, BRITO; ECHENIQUE, R.; DÍAZ RIVERA, H; Y, COLINA MARTINEZ; MANIGLIA MÉRIDA, G. C; & ARRIETA MENDOZA, D. 2019. Determinación de residuos de cloranfenicol y sulfamidas en leches de larga duración, en la ciudad de Maracay , Venezuela. Revista Científica De La Facultad De Ciencias Veterinarias De La Universidad Del Zulia, 28(2), 121 - 128. Recuperado a partir de https://produccioncientificaluz.org/ index.php/cientifica/article/view/29200
VIÑAS, P; BALSALOBRE, N; LÓPEZ-ERROZ, C; HERNÁNDEZ-CÓRDOBA, M. Liquid chromatography with ultraviolet absorbance detection for the analysis of tetracycline residues in honey. J .Chromatogr. A. 1022: 125-129. 2004.
WILSON, JS; OTSUKI, T; MAJUMDSAR, B; Balancing food safety and risk: do drug residue limits affect international trade in beef J .Int. Trade. Econ. Dev. 2: 37-402. 2003.
ZHOU, J; XUE, X; LI, Y; ZHANG, J; CHEN, F; WU, L; CHEN, L; ZHAO, J. Multiresidue determination of tetracycline antibiotics in propolis by using HPLC-UV detection with ultrasonic-assisted extraction and two-step solid phase extraction.Food Chem.115: 1074-1080. 2009.
Published
2021-05-18
How to Cite
1.
Merchán-Castellanos NA, Aguilera-Becerra AM, Manrique WE. Development of a method for detection and quantification of tetracyclineresidues in cheese by high-performance liquid chromatography (HPLC): Desarrollo de un método de detección y cuantificación de residuos de tetraciclina en quesos por cromatografía liquida de alta eficiencia (HPLC). Rev. Cient. FCV-LUZ [Internet]. 2021May18 [cited 2024Nov.1];30(4):180-5. Available from: https://www.produccioncientificaluz.org/index.php/cientifica/article/view/35976
Section
Veterinary Medicine
