Effects of cryopreservation on the spermeshead morphometry in goat semen
Efecto de criopreservación sobre la morfometría de la cabeza espermática en semen de caprinos
Keywords:
Morphometry, sperm, goat, subpopulation
Abstract
The aim of this study was to evaluate the effect of temperature changes during cryopreservation process on the sperm head morphometry in the goat semen. Fifty six ejaculates of 7 male goats of recognized fertility were evaluated by using the Sperm Morphology Analysis (ASMA) to determine the sperm head size and shape: Length (L), Width (W), Area (A), Perimeter (P), Ellipticity (E), Rugosity (R), Elongation (El), and Regularity (RE). FASTCLUS procedure was done to classify the spermatozoa (SPZ) of the data set into a reduced number of subpopulations (SP). Fresh: Subpopulation 1 (SP1) consisted of long and wide spermatozoa (35.12%), SP2 with SPZ of lower ellipticity (36.81%), SP3 in SPZ of average measurements (9.58%) and SP4 in small (18.48%). Cooling: SP1 (32.93%) are SPZ long, SP4 (18.54%) small, SP3 (12.88%) are similar to SP1, SP2 (35.66%) SPZ not hydrodynamic (very wide). Post thawing: SP2 (35.66%) wide SPZ, SP1 (30.92%) long and elliptical (hydrodynamic), SP3 (14.43%) similar to SP1, SP4 (21.53%) they are small cells. The minimal effects of the sperm cryopreservation process on the morphometry in goat semen were observed in the frequency of SPZ distribution in SPs and the characteristics of their forms for each subpopulation; the structure of the SP was maintained after the period of refrigeration and cold storage. In conclusion, cryopreservation did not modify specific parameters such as SPZ distribution within SPs (less than 5% of changes).
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References
ABOAGLA, E.; TERADA, T. Effects of egg yolk during the freezing step preservative on the viability of goat spermatozoa. Theriogenol. 62:1160-1172. 2004.
AGCA, Y.; GILMORE, J.; BYERS, M.; WOODS, E.; LIU, J.; CRITSER, J. Osmotic characteristics of mouse spermatozoa in the presence of extenders and sugars. Biol. Reprod. 67:1493-1501. 2002.
ÁLVAREZ, M.; GARCIA MACIAS, V.; MARTINEZ PASTOR, F.; MARTINEZ, F.; BORRAGAN, S.; MATA., M.; GARDE, JJ.;
ANEL, L.; DE PAZ, P. Effects of cryopreservation on head morphometry and its relation with chromatin status in brown bear (Ursus arctos) spermatozoa. Theriogenol. 70:1498-1506. 2008.
ARAGÃO, F. Otimização da criopreservação seminal e fertilização assistida em cyprinus carpio (Actinopterygii) no semiárido brasileiro. Universidade Estadual Do Ceará. Tese (Doctourado). 93 pp. 2016.
ARRUDA, RP.; BALL, BA.; GRAVANCE, CG.; GARCIA, RP.; LIU, IK. Effects of extenders and cryoprotectants on stallion sperm head morphometry. Theriogenol. 58:252-256. 2002.
ATTIA, S.; KATILA, T.; ANDERSSON, M. The effect of sperm morphology and sire fertility on calving rate of Finnish Ayrshire AI bulls. Reprod. Domest. Anim. 51:54-58. 2016.
BENSON, JD.; WOODS, EJ.; WALTERS, EM.; CRITSER, JK. The cryobiology of spermatozoa. Theriogenol. 78:1682-1699. 2012.
CASEY, PJ.; GRAVANCE, CG.; DAVIS, RO.; CHABOT, DD.; LIU, IK. Morphometric differences in sperm head dimensions of fertile and subfertile stallions. Theriogenol. 47:575-582. 1997.
CAVALCANTE, T. Plasma concentrations of testosterone and fertility of bucks of the Alpine and Boer breed during breeding and nonbreeding season. Faculty of Veterinary Medicine and Animal Science, UNESP, Jaboticabal-SP. Thesis of Grade. 77pp. 013.
CURRY, MR.; KLEINHANS, FW.; WATSON, PF. Measurement of the water permeability of the membranes of boar, ram, and rabbit spermatozoa using concentration dependent self-quenching of an entrapped fluorophore. Cryobiol. 41:167-73. 2000.
DORADO, J. Respuesta a la congelación descongelación del esperma de macho cabrío. Universidad de Córdoba. Facultad de Veterinaria. Tesis Doctoral. 203pp. 2003.
ESTESO, MC.; SOLER, AJ.; FERNÁNDEZ SANTOS, MR.; QUINTERO MORENO, A.; GARDE, JJ. Functional significance of the sperm head morphometric size and shape for determining freezability in Iberian red deer (Cervus elaphus hispanicus) epididymal sperm samples. J. Androl. 27:662-670. 2006.
ESTESO, M.; RODRÍGUEZ, E.; TOLEDANO DÍAZ, A.; CASTAÑO, C.; PRADIEE, J.; LÓPEZ SEBASTIÁN, A.; SANTIAGO MORENO, J. Descriptive analysis of sperm head morphometry in Iberian ibex (Capra pyrenaica): optimum sampling procedure and staining methods using Sperm Class Analyzer®. Anim. Reprod. Sci. 155:42-49. 2015.
GONZÁLEZ, D.; QUINTERO MORENO, A.; GARDE, J.; ESTESO, M.; FERNÁNDEZ SANTOS, M.; RUBIO GUILLÉN, J.; MEJÍA, W.; GONZÁLEZ, Y.; LEÓN, G.; BOHÓRQUEZ, R. Caracterización morfométrica de la cabeza del espermatozoide porcino mediante análisis computarizado (resultados preliminares). Rev. Cientif. FCV-LUZ. XVIII (5): 570-577. 2008.
GRAVANCE, C.; WHITE, C.; ROBERTSON, K.; CHAMPION, Z.; CASEY, P. The effects of cryopreservation on the morphometric dimensions of caprine sperm beads. Anim. Reprod. Sci. 49:37-43. 1997.
GRAVANCE, C.; VISHWANATH, R.; PITT, C.; GARNER, D.; CASEY, P. Effects of cryopreservation on bull sperm head morphometry. J. Androl. 19:704-709. 1998.
GRAVES, JE.; HIGDON, HL.; BOONE, WR.; BLACKHURST, DW. Developing techniques for determining sperm morphology in today’s andrology laboratory. J. Assisted Reprod. Genet. 22: 219-225. 2005.
HERNÁNDEZ CORREDOR, L.; NIVIA OSUNA, A.; HERNÁNDEZ VILLAMIZAR, D.; RUBIO PARADA, J.; QUINTERO MORENO, A. Evaluación de la movilidad espermática de semen caprino criopreservado bajo diferentes medios diluyentes a través del sistema CASA. Rev. Respuesta 2: 16-27. 2013.
HERNÁNDEZ CORREDOR, L.; DORADO, J.; QUINTERO MORENO, A.; ORTIZ, I.; BUZÓN A.; CORZO, M.; HIDALGO, M. Efecto de dos diluyentes a base de lecitina de soya sobre parámetros morfométricos en semen caprino. Rev. SENNOVA 1(1): 30-43. 2014.
HIDALGO, M.; RODRIGUEZ, I.; DORADO, J.M. The effect of cryopreservation on sperm head morphometry in Florida male goat related to sperm freezability. Anim. Reprod. Sci. 100: 61-72. 2001.
HIDALGO, M.; RODRÍGUEZ, I.; DORADO, J.M. Influence of staining and sampling procedures on gota sperm morphometry using the Sperm Class Analyzer. Theriogenol. 66: 996-1003. 2006.
HIDALGO, M.; RODRÍGUEZ, I.; DORADO, J. The effect of cryopreservation on sperm head morphometry in Florida male goat related to sperm freezability. Anim. Reprod. Sci. 100: 61-72. 2007.
JIMÉNEZ RABADÁN, P.; GARCÍA ÁLVAREZ, O.; VIDAL, A.; MAROTO MORALES, A.; INIESTA CUERDA, M.; RAMÓN, M.; DEL OLMO, E.; FERNÁNDEZ SANTOS, R.; GARDE, J.J.; SOLER, A.J. Effects of vitrification on ram spermatozoa using free-egg yolk extenders. Cryobiol. 71: 85-90. 2015.
LASIENE, K.; GEDRIMAS, V.; VITKUS, A.; GLINSKYTE, S.; LASYS, V.; VALANCIUTE, A.; SIENKIEWICZ, W. Evaluation of morphological criteria of sperm quality before in vitro fertilization and intracytoplasmic sperm injection. Pol. J. Vet. Sci. 16:773-785. 2013.
MARCO JIMENEZ, F.; VIUDES DE C, M.; BALASCH, S.; MOCE, E.; SILVESTRE, M.; GOMEZ, E.; VICENTE, J. Morphometric changes in goat sperm heads induced by cryopreservation. Cryobiol. 52:295-304. 2006.
MAROTO MORALES, A. Evaluación objetiva de la morfometría de los espermatozoides de ovino (Ovis aries). Relaciones con la fertilidad. Universidad de Castilla La Mancha. Instituto de Investigación en Recursos Cinegéticos. Tesis Doctoral. 112pp. 2012.
MAROTO MORALES, A.; RAMÓN, M.; GARCÍA ÁLVAREZ, O.; MONTORO, V.; SOLER, AJ.; FERNÁNDEZ SANTOS, MR.; ROLDAN, ERS.; PÉREZ GUZMÁN, MD.; GARDE, JJ. Sperm head phenotype and male fertility in ram semen. Theriogenol. 84:1536-1541. 2015.
MAROTO MORALES, A.; GARCIA ALVAREZ, O.; RAMÓN, M.; MARTINEZ PASTOR, F.; FERNANDEZ SANTOS, MR.; SOLER, J.; GARDE, JJ. Current status and potential of morphometric sperm analysis. Asian J. Androl. 18: 863–870. 2016.
MARTI, J.; APARICIO, I.; GARCÍA HERREROS, M. Head Morphometric changes in cryopreserved ram spermatozoa are related to sexual maturity. Theriogenol. 75: 473-481. 2011.
PEÑA, AI.; LUGILDE, LL.; BARRIO, M.; HERRADON, PG.; QUINTELA, LA. Effects of Equex from different sources on post thaw survival, longevity and intracellular Ca2+concentration of dog spermatozoa. Theriogenol. 59: 1725-1739. 2003.
PEÑA, F.; SARAVIA, F.; GARCÍA HERREROS, M.; NUÑEZ MARTÍNEZ, I.; TAPIA, J.; JOHANNISSON, A.; WALLGREN, M.; RODRIGUEZ MARTÍNEZ, H. Identification of sperm morphometric subpopulations in two different portions of the boar ejaculate and its relation to post thaw quality. J. Androl. 26(6): 716-723. 2005.
QUINTERO MORENO, A.; MIRÓ, J.; RIGAU, T.; RODRÍGUEZ GIL, JE. Identification of sperm subpopulations with specific motility characteristics in stallion ejaculates. Theriogenol. 59: 1973-1990. 2003.
QUINTERO MORENO, A.; RIGAU, T.; RODRÍGUEZ GIL, JE. Regression analyses and motile sperm subpopulation structure study as improving tools in boar semen quality analyses. Theriogenol. 61:673-690. 2004.
QUINTERO MORENO, A.; RIGAU, T.; RODRÍGUEZ GIL, JE. Multivariate cluster analysis regression procedures as tools to identify motile sperm subpopulations in rabbit semen and to predict semen fertility and litter size. Reprod. Domest. Anim. 42:312-319. 2007.
QUINTERO MORENO, A.; GONZÁLEZ, D.; GARDE, J.J.; ESTESO, M.; FERNÁNDEZ SANTOS, M.; CARVALHO, J.L.; MEJÍA, W.; LEÓN, G. Valoración morfométrica de la cabeza del espermatozoide del cerdo doméstico según su edad. Rev. Cientif. FCV-LUZ. XIX (2): 153-156. 2009.
QUINTERO MORENO, A.; RAMÍREZ, M.; NAVA TRUJILLO, H.; HIDALGO, M. Comparison of two histologic stains in the evaluation of sperm head morphometric measurements in frozen thawed bull semen. Acta Microscop. 24(2): 103-110. 2015.
RAMÓN, M.; MARTÍNEZ PASTOR, F.; GARCÍA ÁLVAREZ, O.; MAROTO MORALES, A.; SOLER, J.; JIMÉNEZ RABADÁN, P.; FERNÁNDEZ SANTOS, M.R.; BERNABÉU, R.; GARDE, J. Taking advantage of the use of supervised learning methods for characterization of sperm population structure related with freezabitlity in the Iberian red deer. Theriogenol. 77:1661-1672. 2012.
RAMÓN, M.; PÉREZ GUZMÁN, MD.; JIMÉNEZ RABADÁN, P.; ESTESO, MC.; GARCÍA ÁLVAREZ, O.; MAROTO MORALES, A; ANEL LÓPEZ, L.; SOLER, AJ.; FERNÁNDEZ SANTOS, R.; GARDE, JJ. Sperm Cell Population Dynamics in Ram Semen during the Cryopreservation Process. PLoS ONE 8(3): e59189. 2013a.
RAMÓN, M.; SOLER, A.; ORTIZ, J.; GARCÍA ÁLVAREZ, O.; MAROTO MORALES, A.; ROLDAN, E.; GARDE, J.J. Sperm population structure and male fertility: an intraespecific study of sperm design and velocity in red deer. Biol. Reprod. 89:110. 2013b.
RIJSSELAERE, T.; VAN SOOM, A.; HOFLACK, G.; MAES, D.; DE KRUIF, A. Automated sperm morphometry and morphology analysis of canine semen by the Hamilton Thorne analyzer. Theriogenol. 62: 1292-1306. 2004.
ROS SANTAELLA, JL.; DOMINGUEZ REBOLLEDO, AE.; GARDE, JJ. Sperm flagellum volume determines freezability in red deer spermatozoa. PLoS One 9: e112382. 2014.
RUBIO GUILLÉN, J.; GONZÁLEZ, D.; GARDE, J.J.; ESTESO, M.; FERNÁNDEZ SANTOS, M.; RODRÍGUEZ GIL, J.E.; MADRID BURY, N.; QUINTERO MORENO, A. Effects of cryopreservation on bull spermatozoa distribution in morphometricall y distinct subpopulations. Reprod. Domest. Anim. 42: 354-357. 2007.
SAILER, BL.; JOST, LK.; EVENSON, DP. Bull sperm head morphometry related to abnormal chromatin structure and fertility. Cytometry 24:167-173. 1996.
SINHA, C.; YADAV, S.; YADAV, B.; KUMAR, J.; SINGH, K. Objective assessment of sperm morphometry of Barbari Bucks. Ind. J. Small Rumin. 20(2): 129-131. 2014.
TABAREZ, A. Optimización del protocolo de crioconservación de semen caprino de la raza autóctona en peligro de extinción Blanca de Rasquera. Facultad de Veterinaria. Universidad Autónoma de Barcelona. Tesis Doctoral. 264 pp. 2014.
THOMAS, C.; GARNER, D.; DEJARNETTE, J.; MARSHALL, C. Fluorometric assessments of acrosomal integrity and viability in cryopreserved bovine spermatozoa. Biol. Reprod. 56: 991-998. 1997.
VICENTE FIEL, S.; PALACIN, I.; SANTOLARIA, P.; YANIZ, J. A comparative study of sperm morphometric subpopulations in cattle, goat, sheep and pigs using a computer assisted fluorescence method (CASMA-F). Anim. Reprod. Sci. 139: 182-189. 2013.
AGCA, Y.; GILMORE, J.; BYERS, M.; WOODS, E.; LIU, J.; CRITSER, J. Osmotic characteristics of mouse spermatozoa in the presence of extenders and sugars. Biol. Reprod. 67:1493-1501. 2002.
ÁLVAREZ, M.; GARCIA MACIAS, V.; MARTINEZ PASTOR, F.; MARTINEZ, F.; BORRAGAN, S.; MATA., M.; GARDE, JJ.;
ANEL, L.; DE PAZ, P. Effects of cryopreservation on head morphometry and its relation with chromatin status in brown bear (Ursus arctos) spermatozoa. Theriogenol. 70:1498-1506. 2008.
ARAGÃO, F. Otimização da criopreservação seminal e fertilização assistida em cyprinus carpio (Actinopterygii) no semiárido brasileiro. Universidade Estadual Do Ceará. Tese (Doctourado). 93 pp. 2016.
ARRUDA, RP.; BALL, BA.; GRAVANCE, CG.; GARCIA, RP.; LIU, IK. Effects of extenders and cryoprotectants on stallion sperm head morphometry. Theriogenol. 58:252-256. 2002.
ATTIA, S.; KATILA, T.; ANDERSSON, M. The effect of sperm morphology and sire fertility on calving rate of Finnish Ayrshire AI bulls. Reprod. Domest. Anim. 51:54-58. 2016.
BENSON, JD.; WOODS, EJ.; WALTERS, EM.; CRITSER, JK. The cryobiology of spermatozoa. Theriogenol. 78:1682-1699. 2012.
CASEY, PJ.; GRAVANCE, CG.; DAVIS, RO.; CHABOT, DD.; LIU, IK. Morphometric differences in sperm head dimensions of fertile and subfertile stallions. Theriogenol. 47:575-582. 1997.
CAVALCANTE, T. Plasma concentrations of testosterone and fertility of bucks of the Alpine and Boer breed during breeding and nonbreeding season. Faculty of Veterinary Medicine and Animal Science, UNESP, Jaboticabal-SP. Thesis of Grade. 77pp. 013.
CURRY, MR.; KLEINHANS, FW.; WATSON, PF. Measurement of the water permeability of the membranes of boar, ram, and rabbit spermatozoa using concentration dependent self-quenching of an entrapped fluorophore. Cryobiol. 41:167-73. 2000.
DORADO, J. Respuesta a la congelación descongelación del esperma de macho cabrío. Universidad de Córdoba. Facultad de Veterinaria. Tesis Doctoral. 203pp. 2003.
ESTESO, MC.; SOLER, AJ.; FERNÁNDEZ SANTOS, MR.; QUINTERO MORENO, A.; GARDE, JJ. Functional significance of the sperm head morphometric size and shape for determining freezability in Iberian red deer (Cervus elaphus hispanicus) epididymal sperm samples. J. Androl. 27:662-670. 2006.
ESTESO, M.; RODRÍGUEZ, E.; TOLEDANO DÍAZ, A.; CASTAÑO, C.; PRADIEE, J.; LÓPEZ SEBASTIÁN, A.; SANTIAGO MORENO, J. Descriptive analysis of sperm head morphometry in Iberian ibex (Capra pyrenaica): optimum sampling procedure and staining methods using Sperm Class Analyzer®. Anim. Reprod. Sci. 155:42-49. 2015.
GONZÁLEZ, D.; QUINTERO MORENO, A.; GARDE, J.; ESTESO, M.; FERNÁNDEZ SANTOS, M.; RUBIO GUILLÉN, J.; MEJÍA, W.; GONZÁLEZ, Y.; LEÓN, G.; BOHÓRQUEZ, R. Caracterización morfométrica de la cabeza del espermatozoide porcino mediante análisis computarizado (resultados preliminares). Rev. Cientif. FCV-LUZ. XVIII (5): 570-577. 2008.
GRAVANCE, C.; WHITE, C.; ROBERTSON, K.; CHAMPION, Z.; CASEY, P. The effects of cryopreservation on the morphometric dimensions of caprine sperm beads. Anim. Reprod. Sci. 49:37-43. 1997.
GRAVANCE, C.; VISHWANATH, R.; PITT, C.; GARNER, D.; CASEY, P. Effects of cryopreservation on bull sperm head morphometry. J. Androl. 19:704-709. 1998.
GRAVES, JE.; HIGDON, HL.; BOONE, WR.; BLACKHURST, DW. Developing techniques for determining sperm morphology in today’s andrology laboratory. J. Assisted Reprod. Genet. 22: 219-225. 2005.
HERNÁNDEZ CORREDOR, L.; NIVIA OSUNA, A.; HERNÁNDEZ VILLAMIZAR, D.; RUBIO PARADA, J.; QUINTERO MORENO, A. Evaluación de la movilidad espermática de semen caprino criopreservado bajo diferentes medios diluyentes a través del sistema CASA. Rev. Respuesta 2: 16-27. 2013.
HERNÁNDEZ CORREDOR, L.; DORADO, J.; QUINTERO MORENO, A.; ORTIZ, I.; BUZÓN A.; CORZO, M.; HIDALGO, M. Efecto de dos diluyentes a base de lecitina de soya sobre parámetros morfométricos en semen caprino. Rev. SENNOVA 1(1): 30-43. 2014.
HIDALGO, M.; RODRIGUEZ, I.; DORADO, J.M. The effect of cryopreservation on sperm head morphometry in Florida male goat related to sperm freezability. Anim. Reprod. Sci. 100: 61-72. 2001.
HIDALGO, M.; RODRÍGUEZ, I.; DORADO, J.M. Influence of staining and sampling procedures on gota sperm morphometry using the Sperm Class Analyzer. Theriogenol. 66: 996-1003. 2006.
HIDALGO, M.; RODRÍGUEZ, I.; DORADO, J. The effect of cryopreservation on sperm head morphometry in Florida male goat related to sperm freezability. Anim. Reprod. Sci. 100: 61-72. 2007.
JIMÉNEZ RABADÁN, P.; GARCÍA ÁLVAREZ, O.; VIDAL, A.; MAROTO MORALES, A.; INIESTA CUERDA, M.; RAMÓN, M.; DEL OLMO, E.; FERNÁNDEZ SANTOS, R.; GARDE, J.J.; SOLER, A.J. Effects of vitrification on ram spermatozoa using free-egg yolk extenders. Cryobiol. 71: 85-90. 2015.
LASIENE, K.; GEDRIMAS, V.; VITKUS, A.; GLINSKYTE, S.; LASYS, V.; VALANCIUTE, A.; SIENKIEWICZ, W. Evaluation of morphological criteria of sperm quality before in vitro fertilization and intracytoplasmic sperm injection. Pol. J. Vet. Sci. 16:773-785. 2013.
MARCO JIMENEZ, F.; VIUDES DE C, M.; BALASCH, S.; MOCE, E.; SILVESTRE, M.; GOMEZ, E.; VICENTE, J. Morphometric changes in goat sperm heads induced by cryopreservation. Cryobiol. 52:295-304. 2006.
MAROTO MORALES, A. Evaluación objetiva de la morfometría de los espermatozoides de ovino (Ovis aries). Relaciones con la fertilidad. Universidad de Castilla La Mancha. Instituto de Investigación en Recursos Cinegéticos. Tesis Doctoral. 112pp. 2012.
MAROTO MORALES, A.; RAMÓN, M.; GARCÍA ÁLVAREZ, O.; MONTORO, V.; SOLER, AJ.; FERNÁNDEZ SANTOS, MR.; ROLDAN, ERS.; PÉREZ GUZMÁN, MD.; GARDE, JJ. Sperm head phenotype and male fertility in ram semen. Theriogenol. 84:1536-1541. 2015.
MAROTO MORALES, A.; GARCIA ALVAREZ, O.; RAMÓN, M.; MARTINEZ PASTOR, F.; FERNANDEZ SANTOS, MR.; SOLER, J.; GARDE, JJ. Current status and potential of morphometric sperm analysis. Asian J. Androl. 18: 863–870. 2016.
MARTI, J.; APARICIO, I.; GARCÍA HERREROS, M. Head Morphometric changes in cryopreserved ram spermatozoa are related to sexual maturity. Theriogenol. 75: 473-481. 2011.
PEÑA, AI.; LUGILDE, LL.; BARRIO, M.; HERRADON, PG.; QUINTELA, LA. Effects of Equex from different sources on post thaw survival, longevity and intracellular Ca2+concentration of dog spermatozoa. Theriogenol. 59: 1725-1739. 2003.
PEÑA, F.; SARAVIA, F.; GARCÍA HERREROS, M.; NUÑEZ MARTÍNEZ, I.; TAPIA, J.; JOHANNISSON, A.; WALLGREN, M.; RODRIGUEZ MARTÍNEZ, H. Identification of sperm morphometric subpopulations in two different portions of the boar ejaculate and its relation to post thaw quality. J. Androl. 26(6): 716-723. 2005.
QUINTERO MORENO, A.; MIRÓ, J.; RIGAU, T.; RODRÍGUEZ GIL, JE. Identification of sperm subpopulations with specific motility characteristics in stallion ejaculates. Theriogenol. 59: 1973-1990. 2003.
QUINTERO MORENO, A.; RIGAU, T.; RODRÍGUEZ GIL, JE. Regression analyses and motile sperm subpopulation structure study as improving tools in boar semen quality analyses. Theriogenol. 61:673-690. 2004.
QUINTERO MORENO, A.; RIGAU, T.; RODRÍGUEZ GIL, JE. Multivariate cluster analysis regression procedures as tools to identify motile sperm subpopulations in rabbit semen and to predict semen fertility and litter size. Reprod. Domest. Anim. 42:312-319. 2007.
QUINTERO MORENO, A.; GONZÁLEZ, D.; GARDE, J.J.; ESTESO, M.; FERNÁNDEZ SANTOS, M.; CARVALHO, J.L.; MEJÍA, W.; LEÓN, G. Valoración morfométrica de la cabeza del espermatozoide del cerdo doméstico según su edad. Rev. Cientif. FCV-LUZ. XIX (2): 153-156. 2009.
QUINTERO MORENO, A.; RAMÍREZ, M.; NAVA TRUJILLO, H.; HIDALGO, M. Comparison of two histologic stains in the evaluation of sperm head morphometric measurements in frozen thawed bull semen. Acta Microscop. 24(2): 103-110. 2015.
RAMÓN, M.; MARTÍNEZ PASTOR, F.; GARCÍA ÁLVAREZ, O.; MAROTO MORALES, A.; SOLER, J.; JIMÉNEZ RABADÁN, P.; FERNÁNDEZ SANTOS, M.R.; BERNABÉU, R.; GARDE, J. Taking advantage of the use of supervised learning methods for characterization of sperm population structure related with freezabitlity in the Iberian red deer. Theriogenol. 77:1661-1672. 2012.
RAMÓN, M.; PÉREZ GUZMÁN, MD.; JIMÉNEZ RABADÁN, P.; ESTESO, MC.; GARCÍA ÁLVAREZ, O.; MAROTO MORALES, A; ANEL LÓPEZ, L.; SOLER, AJ.; FERNÁNDEZ SANTOS, R.; GARDE, JJ. Sperm Cell Population Dynamics in Ram Semen during the Cryopreservation Process. PLoS ONE 8(3): e59189. 2013a.
RAMÓN, M.; SOLER, A.; ORTIZ, J.; GARCÍA ÁLVAREZ, O.; MAROTO MORALES, A.; ROLDAN, E.; GARDE, J.J. Sperm population structure and male fertility: an intraespecific study of sperm design and velocity in red deer. Biol. Reprod. 89:110. 2013b.
RIJSSELAERE, T.; VAN SOOM, A.; HOFLACK, G.; MAES, D.; DE KRUIF, A. Automated sperm morphometry and morphology analysis of canine semen by the Hamilton Thorne analyzer. Theriogenol. 62: 1292-1306. 2004.
ROS SANTAELLA, JL.; DOMINGUEZ REBOLLEDO, AE.; GARDE, JJ. Sperm flagellum volume determines freezability in red deer spermatozoa. PLoS One 9: e112382. 2014.
RUBIO GUILLÉN, J.; GONZÁLEZ, D.; GARDE, J.J.; ESTESO, M.; FERNÁNDEZ SANTOS, M.; RODRÍGUEZ GIL, J.E.; MADRID BURY, N.; QUINTERO MORENO, A. Effects of cryopreservation on bull spermatozoa distribution in morphometricall y distinct subpopulations. Reprod. Domest. Anim. 42: 354-357. 2007.
SAILER, BL.; JOST, LK.; EVENSON, DP. Bull sperm head morphometry related to abnormal chromatin structure and fertility. Cytometry 24:167-173. 1996.
SINHA, C.; YADAV, S.; YADAV, B.; KUMAR, J.; SINGH, K. Objective assessment of sperm morphometry of Barbari Bucks. Ind. J. Small Rumin. 20(2): 129-131. 2014.
TABAREZ, A. Optimización del protocolo de crioconservación de semen caprino de la raza autóctona en peligro de extinción Blanca de Rasquera. Facultad de Veterinaria. Universidad Autónoma de Barcelona. Tesis Doctoral. 264 pp. 2014.
THOMAS, C.; GARNER, D.; DEJARNETTE, J.; MARSHALL, C. Fluorometric assessments of acrosomal integrity and viability in cryopreserved bovine spermatozoa. Biol. Reprod. 56: 991-998. 1997.
VICENTE FIEL, S.; PALACIN, I.; SANTOLARIA, P.; YANIZ, J. A comparative study of sperm morphometric subpopulations in cattle, goat, sheep and pigs using a computer assisted fluorescence method (CASMA-F). Anim. Reprod. Sci. 139: 182-189. 2013.
Published
2021-03-16
How to Cite
1.
Hernández-Corredor L, Rubio-Parada J, Quintero-Moreno A. Effects of cryopreservation on the spermeshead morphometry in goat semen: Efecto de criopreservación sobre la morfometría de la cabeza espermática en semen de caprinos. Rev. Cient. FCV-LUZ [Internet]. 2021Mar.16 [cited 2024Nov.1];30(1):27-3. Available from: https://www.produccioncientificaluz.org/index.php/cientifica/article/view/35541
Section
Veterinary Medicine
