Effect of Propolis dye vs Chlorhexidine in the treatment of Periodontal Disease in domestic canines

• Diego Medina-Valarezo Grupo de Investigación de Estudios de Bienestar Animal, Facultad Ciencias de la Salud Eugenio Espejo. Universidad UTE, Sede Occidental, Quito, Ecuador
• Martha Camacho Hospital Veterinario Planeta Vida. Latacunga, Ecuador.
• Manuel García-Herreros Instituto Nacional de Investigação Agrária e Veterinária, I. P. (INIAV, I.P.). Santarém, Portugal
• Andrés Ortega-Ojeda Grupo de Investigación de Estudios de Bienestar Animal, Facultad Ciencias de la Salud Eugenio Espejo. Universidad UTE, Sede Occidental, Quito, Ecuador
• Francisco Angulo-Cubillán Departamento de Sanidad Animal, Facultad de Ciencias Veterinarias, Universidad del Zulia. Maracaibo, Venezuela.

DOI: https://doi.org/10.52973/rcfcv-luz313.art1

Keywords: Bactericidal effect, propolis, chlorhexidine, canine, periodontal disease

Abstract

In the present study the effect of propolis tincture (PT) vs chlorhexidine (C) as a treatment of canine periodontal disease (CPD) was analysed. A therapeutic application in 30 patients with different degrees of CPD was carried out (low, moderate and severe). Samples from the oral cavity were collected for culture and further identification of pathogenic microorganisms involved in the pathological process. Four pathogens of different genera were identified; Staphylococcus spp., Corynebacterium spp., Lactobacillus spp. and Enterobacterias. The cases that responded to treatment were 80 and 3.4 % for PT and C, respectively. C did not demonstrate any efficient action in the treatment of CPD. However, PT demonstrated to be the treatment of choice regardless the pathogen, pathogen associations or the degree of progression of CPD

References

AHN, M.R.; KUMAZAWA, S.; HAMASAKA, T.; BANG, K.S.; NAKAYAMA, T. Antioxidant activity and constituents of propolis collected in various areas of Korea. Agric. Food Chem. 52 (24):7286-92. 2004.

AN, H.J.; LEE, W.R.; KIM, K.H.; KIM, J.Y.; LEE, S.J.; HAN, S.M.; LEE, K.G.; LEE, C.K.; PARK, K.K. Inhibitory effects of bee venom on Propionibacterium acnes-induced inflammatory skin disease in an animal model. Int. J. Mol. Med. 34 (5):1341-8. 2014.

ALBUQUERQUE, C.; MORINHA, F.; REQUICHA, J.; MARTINS, T.; DIAS, I.; GUEDES-PINTO, H.; BASTOS, E.; VIEGAS, C. Canine periodontitis: the dog as an important model for periodontal studies. Vet. J. 191 (3):299-305. 2012.

DAVIS, I.J.; WALLIS, C.; DEUSCH, O.; COLYER, A.; MILELLA, L.; LOMAN, N.; HARRIS, S. A cross-sectional survey of bacterial species in plaque from client owned dogs with healthy gingiva, gingivitis or mild periodontitis. PLoS One. 8 (12): 1-12. 2013.

DAHLÉN, G.; CHARALAMPAKIS, G.; ABRAHAMSSON, I.; BENGTSSON, L.; FALSEN, G. Predominant bacterial species in subgingival plaque in dogs. J. Periodontal Res. 47 (3): 354-64. 2012.

GIL, M.; PERELLI, A.; ALVARADO, R.; ARIAS, Y.; BLUMENTHAL, E. Actividad bacteriostática y bactericida de la tintura de propóleos sobre bacterias enteropatógenas. Rev. Fac. Cs. Salud. 16 (3): 21-25. 2012.

HOLMSTROM, S.E. Veterinary dentistry in senior canines and felines. Vet. Clin. North Am. Small Anim. Pract. 42 (4): 793-808. 2012.

HOTWANI, K.; BALIGA, S.; SHARMA, K. Phytodentistry: use of medicinal plants. J. Complement Integr. Med. 11 (4): 233-51. 2014.

INSTITUTO NACIONAL DE METEOROLOGÍA E HIDROLOGÍA (INAMHI), Ecuador. 2014. Anuario Meteorológico, Nº 51-2011. En Línea: https://www.gob.ec/inamhi 27-05-2021.

KIRAN, M.; ARPAK, N.; UNSAL, E.; ERDOGAN, M.F. The effect of improved periodontal healthon metabolic control in type 2 diabetes mellitus. J. Clin. Periodontol. 32: 266-272. 2005.

KUMAZAWA, S.; BONVEHÍ, J.S.; TORRES, C.; MOK-RYEON, A.; BERMEJO, F.J. Chemical and functional characterisation of propolis collected from East Andalusia (southern Spain). Phytochem. Anal. 24 (6): 608-15. 2013.

LAPENNA, M.; MEDINA, G.; DÍAZ, L.; AGUILLÓN, K.; MARÍN, H. actividad bactericida y fungicida de algunas plantas utilizadas en la medicina tradicional venezolana. Rev. Inst. Nac. Hig. Rafael Rangel. 34 (1): 6-9. 2003.

LEVI, J.R.; BRODY, R.M.; MCKEE-COLE, K.; PRIBITKIN, E.; O’REILLY, R. Complementary and alternative medicine for pediatric otitis media. Int. J. Pediatr. Otorhinolaryngol.77 (6): 926-31. 2013.

LOW, S.B.; PEAK, R.M.; SMITHSON, C.W.; PERRONE, J.; GADDIS, B.; KONTOGIORGOS, E. Evaluation of a topical gel containing a novel combination of essential oils and antioxidants for reducing oral malodor in dogs. Am. J. Vet. Res. 75 (7): 653-7. 2014.

MARSHALL, M.D.; WALLIS, C.V.; MILELLA, L.; COLYER, A.; TWEEDIE, A.D.; HARRIS, S. A longitudinal assessment of periodontal disease in 52 Miniature Schnauzers. BMC Vet. Res. 10 (1):166. 2014.

MUMMOLO, S.; TIERI, M.; TECCO, S.; MATTEI, A.; ABANI, F.; GIUCA, M.; MARZO, G. Clinical evatution of salivary indices and levels Streptococcus mutans and Lactobacillusin patients treated with Occlus Guide. Europ. J. Paedriat. Dent. 15: 367-370. 2014.

PREMOLI, G.; LAGUADO, P.; DÍAZ, N.; ROMERO, C.; VILLARREAL, J.; GONZÁLEZ, A. Uso del propóleo en odontología. A. Odont. Vzlana. 48 (2): 1-13. 2010.[18] RESTREPO, A.I; VELASCO, S.C.; FRANCO, L. Evolution of Explanatory Models of Periodontal Disease Pathogenesis. Rev. Estomat. 17 (2): 52-59. 2009.

SCHMIDLIN, P.R.; ENGLISH, H.; DUNCAN, W.; BELIBASAKIS, G.N.; THUMHEER, T. Antibacterial potential of Manuka honey against three oral bacteria in vitro. Swiss Dent. J. 124 (9): 922-924. 2014.

STATISTICAL PACKAGE FOR THE SOCIAL SCIENCES (SPSS). SPSS Inc. Headquarters, 733 S. Wacker Drive, 11th floor. Chicago, USA. 2005