Phenotypic and Molecular Detection of Methicillin Resistance in S. aureus

  • Maribel Castellano González Chair of General Bacteriology. School of Bioanalysis. University of Zulia-Venezuela
  • Armindo Perozo Mena Chair of Professional Practice of Bacteriology. School of Bioanalysis. University of Zulia-Venezuela. Center of Bacteriological Reference University Hospital of Maracaibo. Venezuela.
  • Rosana Vivas Vega Escuela de Bioanálisis. Universidad del Zulia-Venezuela
Keywords: S. aureus, methicillin resistance, phenotypic methods, molecular methods

Abstract

Detecting methicillin resistance is complicated due to the heterogeneity of its phenotypic expression, making its detection difficult in the laboratory; this has led to the development of several techniques to increase its expression in vitro. Four techniques for detecting methicillin resistance were evaluated: the disk diffusion method with oxacillin (OX, 1 μg) and cephoxitin (FOX, 30 μg); the screening test, minimum inhibitory concentration (MIC) and detection of PBP2a, using the presence of mecA gen as a reference method; 286 strains of S. aureus, were processed. The sensibility (SEN), specificity (SPE), positive predictive value (PPV), negative predictive value (NPV) and efficiency (EFC) of each method were determined. A total of 50 oxacillin resistant, PBP2a positive (mecA positive) strains were obtained. Sensibility of the OX disk was 99.14%; and of the FOX disk was 100%. The SEN, PPV, NVP and EFC of the other methods were 100%. All the tests, except the OX disk diffusion method (99.14% of ESP), were 100% specific.

Published
2008-06-07
How to Cite
1.
Castellano González M, Perozo Mena A, Vivas Vega R. Phenotypic and Molecular Detection of Methicillin Resistance in S. aureus. Kasmera [Internet]. 2008Jun.7 [cited 2024May12];36(1):28-. Available from: https://www.produccioncientificaluz.org/index.php/kasmera/article/view/4820
Issue
Vol. 36 No. 1 (2008)
Section
Original Articles

Copyright (c) 2008 Maribel Castellano González, Armindo Perozo Mena, Rosana Vivas Vega

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