Nuclear and cytoplasmic expressions of the receptor for advanced glycation end products (RAGE) in the rat central nervous system.

Expresión nuclear y citoplasmática del receptor para compuestos de glicosilación avanzada en el sistema nervioso central de la rata.

• Jesús Mosquera-Sulbarán Universidad del Zulia, Maracaibo, Venezuela. https://orcid.org/0000-0002-1496-5511
• Adriana Pedreáñez Universidad del Zulia, Maracaibo, Venezuela. https://orcid.org/0000-0002-3937-0469
• Yenddy Carrero Universidad del Zulia, Maracaibo, Venezuela. https://orcid.org/0000-0003-4050-4468
• Catherina Peña Universidad del Zulia, Maracaibo, Venezuela. https://orcid.org/0009-0009-2824-2566

DOI: https://doi.org/10.54817/IC.v64n4a7

Keywords: RAGE, ligands, nucleus, cerebral cortex, cerebellum

Abstract

The receptor for advanced glycation end products (RAGE) is a transmembrane protein involved in the induction of inflammatory processes and oxidative stress after interacting with its ligands on the cell surface. Localization on the cell surface is necessary for interaction with the ligands. This study aimed to determine the expression of RAGE in different parts of the normal rat brain and cerebellum using the immunofluorescence technique. Several cerebral cortex layers (molecular/granular layers: M/GL; pyramidal layer: PL) and the hypothalamus were analyzed, as well as the molecular layer (CML) and the granular layer (CGL) of the cerebellum. Cells with RAGE-positive nuclei were generally observed in the brain’s cerebral cortex and cerebellum. In the M/GL, cells with different degrees of positivity in the nucleus and cytoplasm accompanied by RAGE-positive material in the adjacent extracellular space were observed, and RAGE- positive material in the neuropile. Pyramidal neurons presenting various degrees of nuclear RAGE-positive material budding and cells with different degrees of nuclear and cytoplasmic positivity were observed in PL. The hypothalamus showed a high number of cells with RAGE-positive granules adjacent to the nucleus and in the cytoplasm; nuclei remained negative. Many positive nuclei were observed in CML; they were scarce in CGL. These data suggest the storage of RAGE at the nuclear and cytoplasmic levels in healthy rats and hypothesize the possible translocation of this molecule to the cell surface in pathological conditions.

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