Capacidad pro-apoptótica in vitro de Valeriana rígida y Valeriana decussata sobre una línea celular de cáncer de mama.
In vitro pro-apoptotic capacity of Valerian rigid and Valerian decussata in cancer cells.
Keywords:
Bcl-2, BAX, plant extract, Valeriana, breast cancer
Abstract
Cancer represents a worldwide public health problem, with high incidence and mortality rates in developed and undeveloped countries. Currently, therapeutic alternatives of natural origin are being evaluated with the purpose of establishing more efficient and less invasive treatments. Apoptosis is the type of programmed death cancer cells undergo during treatment with anti-neoplastic drugs. Therefore, the aim of this research was to evaluate in vitro the pro-apoptotic and cytotoxic capacity of valerian extracts on a breast cancer cell line (MCF-7). In this study, MCF7 cells were cultured and treated with different concentrations of the extracts of the root, leaves and stems of Valeriana rígida and Valeriana decussata. Cell viability was assessed by the MTT assay. Quantitative reverse transcription PCR assays were used for the determination of gene expression of anti- and proapoptotic proteins (Bax, Bcl-2, p53). Different concentrations of the extracts (10 –8 to 10 –1 mg/mL) decreased cell viability (proliferation) in a concentration-dependent manner. These extracts induced gene expression of Bax and Bcl-2 proteins but not of p53. The expression of Bax was higher than that of Bcl-2, causing an elevated Bax/Bcl-2 ratio (proapoptotic condition). In conclusion, it was determined that Valeriana decussata and Valeriana rígida extracts have a viability (proliferation) reducing effect on the MCF-7 breast cancer cell line, probably mediated by altering the ratio of Bax and Bcl-2 proteins linked to apoptosis.
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References
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Kakehashi A, Kato A, Ishii N, Wei M, Morimura K, Fukushima S, Wanibuchi H. Valerian inhibits rat hepatocarcinogenesis by activating GABA(A) receptor-mediated signaling. PLoS One 2014; 9(11):e113610. doi: 10.1371/journal.pone.0113610. 31.
Condori M, Oviedo M. Evaluación del rfecto antiproliferativo y apoptótico del extracto en acetato de rtilo de las hojas de Annona muricata (Guanábana) sobre células cancerígenas (pc-3) y células epiteliales sanas de próstata humana (HPrEC), Boston, MA. 2016. MOSAICO. Arequipa; s.n; 2017; 19-21.
GLOBOCAN. Informe de OMS [Internet]. 2018 Available from: https://www.redaccionmedica.ec/secciones/salud-publica/ecuador-registra-28-058-nuevos-casos-de-c-ncer-seg-n-informe-de-oms-92834.
Martínez A, Gómez L, Rodríguez C. La muerte celular: un proceso indispensable para la vida. Ciencia UANL 2018; 21(87). Disponible: http://cienciauanl.uanl.mx/?p=7517.
Mohan H. Patología. 6ª ed. Médica Panamericana 2012: 45–49. Pérez-Machado J, Lie-Concepción A. Apoptosis, mecanismo de acción. Medimay 2012;18(2):15 p. Disponible en: http:// revcmhabana.sld.cu/index.php/rcmh/arti- cle/view/572.
Murray M, Birdsall T, Pizzomo J, Reilly P. La Curación del Cáncer: Métodos Naturales - Michael Murray, Tim Birdsall, Joseph E. Pizzorno, Paul Reilly. Google Libros. 2004: 127–130 p. Disponible: https:// books.google.es/books?hl=es&lr=&id=2PKw5JFAvgC&oi=fnd&pg=PA9&dq=tratamientos+naturales+contra+el+cancer&ots=k_GQmqiKYZ&sig=wEc6G0-tQSKoL5E8e7mo4-L4gT8#v=onepage&q=tratamientosnaturalescontraelcancer&f=false
S.N Fundación Salud y Naturaleza. Libro Blanco de los herbolarios y las plantas medicinales 2007.
DEEL. DEEL - Diccionario Etimológico Español en Línea [Internet]. 2020 . Disponible: http://etimologias.dechile.net/
Kutschker A. Revisión del género Valeriana (Valerianaceae) en Sudamérica austral. Vol. 68, Gayana - Botanica. Universidad de Concepcion; 2011. 244–296.
Li X, Chen T, Lin S, Zhao J, Chen P, Ba Q, Guo H, Liu Y, Li J, Chu R, Shan L, Zhang W, Wang H. Valeriana jatamansi constituent IVHD-valtrate as a novel therapeutic agent to human ovarian cancer: in vitro and in vivo activities and mechanisms. Curr Cancer Drug Targets 2013;13(4):472-483. doi:10.2174/1568009611313040009.
Centro de Investigación del Cáncer. Nuevos tratamientos. Estrategias terapéuticas derivadas de la Biología Molecular. Centro de investigación del Cáncer - Comprehensive Cancer Center Research [Internet]. 2017. Disponible en: http://www.cicancer. org/es/nuevos-tratamientos-estrategias-terapeuticas-derivadas-de-la-biologia-mole- cular
Honma T, Shiratani N, Banno Y, Shiratani N, Banno Y, Kataoka T, Kimura R, Sato I, Endo Y, Kita K, T Suzuki T, Takayanag T. Seeds of Centranthus ruber and Valeriana officinalis contain conjugated linolenic acids with reported antitumor effects. J Oleo Sci 2019;68(5):481-491. doi:10.5650/ jos.ess19007
Han R, Nusbaum O, Chen X, Zhu Y. Valeric acid suppresses liver cancer development by acting as a novel HDAC inhibitor. Mol Ther Oncolytics 2020;19:8-18. Published 2020 Aug 29. doi:10.1016/j.omto.2020.08.017.
Chen L, Feng D, Qian Y, Cheng X, Song H, Zhang X, Wu Y, Huawei L, Liu Q, Cheng G, Yang B, Gu M. Valtrate as a novel therapeutic agent exhibits potent anti-pancreatic cancer activity by inhibiting Stat3 signaling. Phytomedicine 2021;85:153537. doi:10.1016/j.phymed.2021.153537.
Matsumoto T, Kitagawa T, Imahori D, Yoshikawa H, Okayama M, Kobayashi M, Kojima N, Yamashita M, Watanabe T. Cell death-inducing activities via 2Hsp inhibition of the sesquiterpenes isolated from Valeriana fauriei. J Nat Med 2021;75(4):942- 948. doi:10.1007/s11418-021-01543-9.
Shi F, Li Y, Han R, Alan F, Ronghua W, Olivia N, Qin Q, Xinyi C, Li H, Yong Z. Valerian and valeric acid inhibit growth of breast cancer cells possibly by mediating epigenetic modifications. Sci Rep 2021;11(1):2519. doi:10.1038/s41598-021-81620-x.
Farshori N, Saad E, Mohammad M, Musarrat J, Ali A, Ahmed M. Anticancer activity of Petroselinum sativum seed extracts on MCF-7 human breast cancer cells. Asian Pacific J Cancer Prev 2013; 14. doi:10.7314/ APJCP.2013.14.10.5719.
Purelink®. PureLink TM RNA Mini Kit. Cell [Internet]. 2010;(12183020):1–4. Disponible en: https://tools.thermofisher.com/content/sfs/manuals/purelink_rna_mini_ kit_man.pdf
Thermo ScientificTM. Maxima First Strand cDNA Synthesis Kit for RT-qPCR, with dsDNase. 2019. Disponible en: https:// www.thermofisher.com/order/catalog/product/K1671?SID=srch-srp-K1671#/ K1671?SID=srch-srp-K1671.
Vaddavalli PL, Schumacher B. The p53 network: cellular and systemic DNA damage responses in cancer and aging [published online ahead of print, 2022 Mar 25]. Trends Genet 2022;S0168-9525(22)00037-3. doi: 10.1016/j.tig.2022.02.010
Ugalde M, Reza V, González-Trujano ME, Avula B, Khan IA, Navarrete A. Isobolographic analysis of the sedative interaction between six central nervous system depressant drugs and Valeriana edulis hydroalcoholic extract in mice. J Pharm Pharmacol 2005;57(5):631- 639. doi:10. 1211/0022357056000.
Garrido J. Análisis por cromatografía líquida de alta resolución de ácido valerénico o sus derivados en extracto de hojas y raíz de valeriana (Valeriana prionophylla Standl.) Universidad de San Carlos de Guatemala. USAC.2007; 38p. Disponible en: http://biblioteca.usac.edu.gt/tesis/06/06_2611.pdf
Navarro E, Ginebra M. Desarrollo y Caracterización de Materiales Biodegradables para Regeneración Ósea [Internet]. Catalunya; 2005 Disponible en: https://upcommons.upc.edu/bitstream/ handle/2117/93360/05Mnt05de11.pdf? sequence=5&isAllowed=y
Amiri A, Namavari M, Rashidi M, Fahmidehkar MA, Seghatoleslam A. Inhibitory effects of Cyrtopodion scabrum extract on growth of human breast and colorectal cancer cells. Asian Pac J Cancer Prev 2015;16(2):565-570. doi:10.7314/ apjcp.2015.16.2.565.
Flores AMC, Martínez BM., Ruiz V, Reyes, Leyva. J. Evaluación in vitro de la actividad citotóxica y antitumoral de plantas medicinales en Cuetzalan del Progreso, Puebla, México. Polibotánica [Internet]. 2019;0(46):113–35.Disponible en: http:www.polibotanica.mx.
Páez-Hernández G, Espinosa-Andrews H, Castillo-Herrera G, Herrera-Rodríguez S. Uso de aceites esenciales como agentes quimiopreventivos contra el cáncer colorrectal. RevSalJal 2020; 6 (3):199-205. 27.
Chuaqui R, Cuello M, Emmert-Buck M. Inactivación de genes supresores de tumores en la carcinogénesis del cuello uterino. Rev Med Chil 1999.];127(12):1501– 12. Disponible en: https://scielo.coni cyt.cl/scielo.php?script=sci_arttext& pid=S003498871999001200014&l-ng=es&nrm=iso&tlng=es.
Hussain A, Sharma C, Khan S, Shah K, Haque S. Aloe vera inhibits proliferation of human breast and cervical cancer cells and acts synergistically with cisplatin. Asian Pac J Cancer Prev 2015;16(7):2939-2946. doi:10.7314/apjcp.2015.16.7.293.
Kale J, Osterlund EJ, Andrews DW. BCL-2 family proteins: changing partners in the dance towards death. Cell Death Differ 2018;25(1):65-80. doi:10.1038/cdd. 2017.186.
Kakehashi A, Kato A, Ishii N, Wei M, Morimura K, Fukushima S, Wanibuchi H. Valerian inhibits rat hepatocarcinogenesis by activating GABA(A) receptor-mediated signaling. PLoS One 2014; 9(11):e113610. doi: 10.1371/journal.pone.0113610. 31.
Condori M, Oviedo M. Evaluación del rfecto antiproliferativo y apoptótico del extracto en acetato de rtilo de las hojas de Annona muricata (Guanábana) sobre células cancerígenas (pc-3) y células epiteliales sanas de próstata humana (HPrEC), Boston, MA. 2016. MOSAICO. Arequipa; s.n; 2017; 19-21.
Published
2022-11-10
How to Cite
Williams Ibarra, J., Carrero, Y., Vargas, J. H., & Acosta , M. (2022). Capacidad pro-apoptótica in vitro de Valeriana rígida y Valeriana decussata sobre una línea celular de cáncer de mama.: In vitro pro-apoptotic capacity of Valerian rigid and Valerian decussata in cancer cells. Investigación Clínica, 63(4), 376-387. https://doi.org/10.54817/IC.v63n4a05
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Articles
