Efecto del propóleo sobre la actividad de la piruvato quinasa y la superóxido dismutasa en el daño tisular inducido por doxorrubicina: análisis de acoplamiento molecular

• Seval Yilmaz Firat University, Faculty of Veterinary Medicine, Department of Biochemistry. Elazig, Türkiye
• Emre Kaya Firat University, Faculty of Veterinary Medicine, Department of Biochemistry. Elazig, Türkiye
• Harun Yonar Selcuk University, Faculty of Veterinary Medicine, Deparment of Biostatistics. Konya, Türkiye
• Harun Uslu Firat University, Faculty of Pharmacy, Department of Pharmaceutical Professional Sciences, Department of Pharmaceutical Chemistry. Elazig, Türkiye

DOI: https://doi.org/10.52973/rcfcv-e34311

Palabras clave: Doxorrubicina, toxicidad, piruvato quinasa, superóxido dismutasa, acoplamiento molecular

Resumen

El estudio tuvo como objetivo, evaluar el efecto del propóleo sobre la piruvato quinasa (PK), que es una enzima clave en la glucólisis y la superóxido dismutasa (SOD), una enzima antioxidante sobre la toxicidad inducida por DOX en diferentes tejidos. Mediante el acoplamiento molecular, analizamos cómo afectaba el propóleo a las enzimas responsables de la glucólisis y el sistema antioxidante. No hubo solicitud en el primer grupo (control). El segundo grupo recibió 100 mg·kg^-1 día de propóleo por sonda gástrica durante 7 días, el tercer grupo recibió una dosis única de 20 mg·kg^-1 de DOX intraperitoneal y el cuarto grupo propóleo+DOX. Dos días antes de la administración de DOX, se inició la aplicación de propóleo, que duró siete días. Se determinaron las actividades de PK y SOD en tejidos de hígado, corazón, riñón y testículos, y se aplicó acoplamiento molecular para ratificar la actividad de algunos componentes del propóleo (éster fenetílico del ácido cafeico (CAPE) y quercetina) sobre las enzimas PK y SOD. Cuando se comparó el grupo DOX con el grupo de control, se encontró una disminución en las actividades de PK y SOD, y se encontró una diferencia significativa en las actividades de PK y SOD. La administración de DOX disminuyó las actividades de PK y SOD de los tejidos del hígado, el corazón, los riñones y los testículos. En conclusión, el presente estudio revela que DOX interrumpe la glucólisis en tejidos de rata. Se demostró que los compuestos CAPE y quercetina interactúan de manera similar con los ligandos cocristalinos de PK y SOD. Además, cuando se examinaron los tipos de interacción de estos compuestos, especialmente en PK, y las puntuaciones de acoplamiento obtenidas, se puede decir que muestran mayor afinidad que DOX.

Citas

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