Sensitivity of Bacilloscopy, Culture and ELISA Methods to Diagnose Lung Tuberculosis, in Patients From Vargas State-Venezuela

  • J Betancourt Chair of Clinical Bacteriology. School of Sciences, Department of Bioanalysis. Oriente University.
  • N Ruiz Department of Bioanalysis. Oriente University.
  • P Cruces Department of Bioanalysis. Oriente University.
  • W Velásquez Physiology Chair. School of Sciences, Department of Bioanalysis. Oriente University.
Keywords: Tuberculosis, Mycobacterium tuberculosis, sensitivity, bacilloscopy, culture, ELISA

Abstract

This research was conducted with the objective of evaluating sensitivity in diagnosing lung tuberculosis, through bacilloscopy, culture and ELISA methods in respiratory symptomatic patients who attended the Neumonology  consultation Department from Sanitary District N° 6, La Guaira, Vargas State-Venezuela. To achieve this, two extended sputum samples were carried  out, which were stained by the Ziehl-Neelsen method (Bacilloscopy). Each sample was sown using the method proposed by Ogawa- Kudoh. A blood sample was taken from the patients with positive sputum samples by bacilloscopy and culture methods to detect antibodies for M. tuberculosis. Out of the 200 samples analyzed to diagnose lung tuberculosis, 20 (10,0%) proved positive through the culture method, and only 11 (5,5%) were serum-positive by the ELISA method. The culture, bacilloscopy, and ELISA methods showed a sensitivity of 100, 65 and 55 % respectively. These results suggest that the Ogawa-Kudoh culture method is much more sensitive; therefore, this method gives more reliable and accurate results, especially in samples with low bacillus density.

Author Biography

N Ruiz, Department of Bioanalysis. Oriente University.
Licenciados en Bioanálisis.
Published
2002-11-06
How to Cite
1.
Betancourt J, Ruiz N, Cruces P, Velásquez W. Sensitivity of Bacilloscopy, Culture and ELISA Methods to Diagnose Lung Tuberculosis, in Patients From Vargas State-Venezuela. Kasmera [Internet]. 2002Nov.6 [cited 2024May24];30(2):137-44. Available from: https://www.produccioncientificaluz.org/index.php/kasmera/article/view/4701
Issue
Vol. 30 No. 2 (2002)
Section
Original Articles

Copyright (c) 2002 J Betancourt, N Ruiz, P Cruces, W Velásquez

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