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Recibido el 19-02-2020 . Aceptado el 13-10-2020 * Autor de correspondencia. Correo electrónico: kolimapena@gamil.com Technical note / Nota Técnica Respuesta agroproductiva de Zea mayz L. con la aplicación foliar de VIUSID agro ® Agroproductive response of Zea mayz L. with the foliar application of VIUSID agro ® Resposta agroprodutiva de Zea mayz L. com a aplicação foliar de VIUSID agro ® Kolima Peña 1,2* , Alexander Calero-Hurtado 2 , Dilier Olivera- Viciedo 2 , Juan Carlos Rodríguez 1 , Thiago Fernandes 2 , Rainel García 1 y Gabriela Ajila 2 1 Universidad de Sancti Spíritus José Martí Pérez. Avenida de los Mártires 360, Sancti iVBORw0KGgoAAAANSUhEUgAAABAAAAAQCAIAAACQkWg2AAABL0lEQVR4nGP5//8/Awzce7f1 /tvtr76cg4uI8RgpCnsqCXnDRVgg1Ndfzw/fq3j//TYDKgBqBqJbr1bZKnVws0lCNQBV77gR /+vvl0jDY1dezN1/s1eETwBZG9AgoAIPjYVAPSANQLOBqhnwAqACoDKgHhagu+EumXxQC00d GzMP3CygMqBiFqAv4dK59tcgTgK6DS745OOhkw9bINqAilmQwwQZAG0D+gQYShby1UYyBSce tkDCgAW/04EqTjxsdVadAtHAAA9W/HqQuYQ1AF2FogHIx+UNiGqgH+6/2wbnsgBjHqsGYIhB GMBQOvdkAoQNVMwCTCfAmIdExfLzVkASGDgQBhoQ5FQFKgb5AZhOIEkDj0+AMQhUxgDxNDCF AOMca+KDm42S+OB6iEneAKkQp/ZDwx7EAAAAAElFTkSuQmCC Spíritus, Cuba. Correos electrónicos: (KP) kolimapena@gamil.com ; (JR) jcarlos@uniss. iVBORw0KGgoAAAANSUhEUgAAABAAAAAQCAIAAACQkWg2AAABL0lEQVR4nGP5//8/Awzce7f1 /tvtr76cg4uI8RgpCnsqCXnDRVgg1Ndfzw/fq3j//TYDKgBqBqJbr1bZKnVws0lCNQBV77gR /+vvl0jDY1dezN1/s1eETwBZG9AgoAIPjYVAPSANQLOBqhnwAqACoDKgHhagu+EumXxQC00d GzMP3CygMqBiFqAv4dK59tcgTgK6DS745OOhkw9bINqAilmQwwQZAG0D+gQYShby1UYyBSce tkDCgAW/04EqTjxsdVadAtHAAA9W/HqQuYQ1AF2FogHIx+UNiGqgH+6/2wbnsgBjHqsGYIhB GMBQOvdkAoQNVMwCTCfAmIdExfLzVkASGDgQBhoQ5FQFKgb5AZhOIEkDj0+AMQhUxgDxNDCF AOMca+KDm42S+OB6iEneAKkQp/ZDwx7EAAAAAElFTkSuQmCC edu.cu ; (RG) rainel@uniss.edu.cu iVBORw0KGgoAAAANSUhEUgAAABAAAAAQCAIAAACQkWg2AAABL0lEQVR4nGP5//8/Awzce7f1 /tvtr76cg4uI8RgpCnsqCXnDRVgg1Ndfzw/fq3j//TYDKgBqBqJbr1bZKnVws0lCNQBV77gR /+vvl0jDY1dezN1/s1eETwBZG9AgoAIPjYVAPSANQLOBqhnwAqACoDKgHhagu+EumXxQC00d GzMP3CygMqBiFqAv4dK59tcgTgK6DS745OOhkw9bINqAilmQwwQZAG0D+gQYShby1UYyBSce tkDCgAW/04EqTjxsdVadAtHAAA9W/HqQuYQ1AF2FogHIx+UNiGqgH+6/2wbnsgBjHqsGYIhB GMBQOvdkAoQNVMwCTCfAmIdExfLzVkASGDgQBhoQ5FQFKgb5AZhOIEkDj0+AMQhUxgDxNDCF AOMca+KDm42S+OB6iEneAKkQp/ZDwx7EAAAAAElFTkSuQmCC . 2 Universidad São Paulo (UNESP), Escuela de Agricultura y Ciencias Veterinarias, Jaboticabal, SP− Brasil. Correos electrónicos: (AC) iVBORw0KGgoAAAANSUhEUgAAABAAAAAQCAIAAACQkWg2AAABL0lEQVR4nGP5//8/Awzce7f1 /tvtr76cg4uI8RgpCnsqCXnDRVgg1Ndfzw/fq3j//TYDKgBqBqJbr1bZKnVws0lCNQBV77gR /+vvl0jDY1dezN1/s1eETwBZG9AgoAIPjYVAPSANQLOBqhnwAqACoDKgHhagu+EumXxQC00d GzMP3CygMqBiFqAv4dK59tcgTgK6DS745OOhkw9bINqAilmQwwQZAG0D+gQYShby1UYyBSce tkDCgAW/04EqTjxsdVadAtHAAA9W/HqQuYQ1AF2FogHIx+UNiGqgH+6/2wbnsgBjHqsGYIhB GMBQOvdkAoQNVMwCTCfAmIdExfLzVkASGDgQBhoQ5FQFKgb5AZhOIEkDj0+AMQhUxgDxNDCF AOMca+KDm42S+OB6iEneAKkQp/ZDwx7EAAAAAElFTkSuQmCC alexcalero34@gmail.com iVBORw0KGgoAAAANSUhEUgAAABAAAAAQCAIAAACQkWg2AAABL0lEQVR4nGP5//8/Awzce7f1 /tvtr76cg4uI8RgpCnsqCXnDRVgg1Ndfzw/fq3j//TYDKgBqBqJbr1bZKnVws0lCNQBV77gR /+vvl0jDY1dezN1/s1eETwBZG9AgoAIPjYVAPSANQLOBqhnwAqACoDKgHhagu+EumXxQC00d GzMP3CygMqBiFqAv4dK59tcgTgK6DS745OOhkw9bINqAilmQwwQZAG0D+gQYShby1UYyBSce tkDCgAW/04EqTjxsdVadAtHAAA9W/HqQuYQ1AF2FogHIx+UNiGqgH+6/2wbnsgBjHqsGYIhB GMBQOvdkAoQNVMwCTCfAmIdExfLzVkASGDgQBhoQ5FQFKgb5AZhOIEkDj0+AMQhUxgDxNDCF AOMca+KDm42S+OB6iEneAKkQp/ZDwx7EAAAAAElFTkSuQmCC , (DO) dilierolvi@gmail.com, , (TF) thiagofeliph@hotmail. iVBORw0KGgoAAAANSUhEUgAAABAAAAAQCAIAAACQkWg2AAABL0lEQVR4nGP5//8/Awzce7f1 /tvtr76cg4uI8RgpCnsqCXnDRVgg1Ndfzw/fq3j//TYDKgBqBqJbr1bZKnVws0lCNQBV77gR /+vvl0jDY1dezN1/s1eETwBZG9AgoAIPjYVAPSANQLOBqhnwAqACoDKgHhagu+EumXxQC00d GzMP3CygMqBiFqAv4dK59tcgTgK6DS745OOhkw9bINqAilmQwwQZAG0D+gQYShby1UYyBSce tkDCgAW/04EqTjxsdVadAtHAAA9W/HqQuYQ1AF2FogHIx+UNiGqgH+6/2wbnsgBjHqsGYIhB GMBQOvdkAoQNVMwCTCfAmIdExfLzVkASGDgQBhoQ5FQFKgb5AZhOIEkDj0+AMQhUxgDxNDCF AOMca+KDm42S+OB6iEneAKkQp/ZDwx7EAAAAAElFTkSuQmCC com iVBORw0KGgoAAAANSUhEUgAAABAAAAAQCAIAAACQkWg2AAABL0lEQVR4nGP5//8/Awzce7f1 /tvtr76cg4uI8RgpCnsqCXnDRVgg1Ndfzw/fq3j//TYDKgBqBqJbr1bZKnVws0lCNQBV77gR /+vvl0jDY1dezN1/s1eETwBZG9AgoAIPjYVAPSANQLOBqhnwAqACoDKgHhagu+EumXxQC00d GzMP3CygMqBiFqAv4dK59tcgTgK6DS745OOhkw9bINqAilmQwwQZAG0D+gQYShby1UYyBSce tkDCgAW/04EqTjxsdVadAtHAAA9W/HqQuYQ1AF2FogHIx+UNiGqgH+6/2wbnsgBjHqsGYIhB GMBQOvdkAoQNVMwCTCfAmIdExfLzVkASGDgQBhoQ5FQFKgb5AZhOIEkDj0+AMQhUxgDxNDCF AOMca+KDm42S+OB6iEneAKkQp/ZDwx7EAAAAAElFTkSuQmCC (GA) gabita31_89@hotmail.com . Resumen Los promotores del crecimiento pueden ser una alternativa para incrementar el crecimiento y la producción de los cultivos de importancia agrícola como el maíz. Para determinar el efecto del VIUSID agro ® en la respuesta agroproductiva del maíz, se diseñaron dos experimentos en años consecutivos. Se realizó un diseño experimental en bloques al azar para evaluar cuatro tratamientos, VIUSID agro ® (0,1; 0,3 y 0,5 L.ha -1 ) y un control, con tres repeticiones. Se evaluaron diferentes variables de crecimiento y desarrollo del cultivo incluido el rendimiento agrícola. Las dosis de mejor comportamiento en la mayoría de las variables en los dos experimentos fueron 0,3 y 0,5 L.ha -1 . En el rendimiento agrícola en ambos experimentos los tratamientos de mejor comportamiento fueron las dosis 0,3 y 0,5 L.ha -1 con un incremento promedio respecto al control de 24,55 y 45,06 %, respectivamente. Por lo que el VIUSID agro ® favoreció la respuesta agroproductiva del maíz. Palabras claves: aminoácido, maíz, granos, promotor del crecimiento. 574 Esta publicación científica en formato digital es continuación de la Revista Impresa: Depósito legal pp 196802ZU42, ISSN 0378-7818. Rev. Fac. Agron. (LUZ). 2021, 38(3): 573-584. Julio-Septiembre. Peña et al. ISSN 2477-9407 Abstract Growth promoters can be an alternative to increase the growth and production of crops of agricultural importance such as corn. To determine the effect of VIUSID agro ® on the agroproductive response of corn, two experiments were designed in consecutive years. In both, a randomized block experimental design was carried out to evaluate four treatments, VIUSID agro ® (0.1, 0.3 and 0.5 L.ha −1 ) and a control, with three replications. Different variables of the growth and development of the crop, including agricultural yield were evaluated. The best performance doses in the several variables of two experiments were 0.3 and 0.5 L.ha −1 . The treatments with the best performance in the agricultural yield in both experiments were the doses 0.3 and 0.5 L.ha −1 with an average increase compared to the control of 24.55 and 45.06 % respectively. Therefore, VIUSID agro ® favored the agro−productive response of corn. Keywords: amino acid, corn, grains, growth promoter. Resumo Os promotores de crescimento podem ser uma alternativa para aumentar o crescimento e a produção de culturas de importância agrícola como o milho. Para determinar o efeito do VIUSID agro ® na resposta agroprodutiva do milho, dois experimentos foram realizados em anos consecutivos. O delineamento experimental foi em blocos casualizados para avaliar quatro tratamentos, VIUSID agro ® (0,1; 0,3 e 0,5 L.ha -1 ) e uma testemunha, com três repetições. Foram avaliadas diferentes variáveis de crescimento e desenvolvimento da cultura, incluindo a produtividade agrícola. As doses com melhor desempenho na maioria das variáveis nos dois experimentos foram 0,3 e 0,5 L.ha -1 . Na produtividade agrícola em ambos os experimentos os tratamentos com melhor desempenho foram as doses 0,3 e 0,5 L.ha -1 com um aumento médio em relação ao controle de 24,55 e 45,06 %, respectivamente. Portanto, VIUSID agro ® favoreceu a resposta agroprodutiva do milho. Palavras-chave: aminoácido, milho, grãos, promotor de crescimento. Introducción El maíz (Zea mays L.) es uno de los cultivos predominantes en los mercados internacionales, es el alimento básico para más de 200 millones de personas y se estima que este número crezca a medida que la población mundial alcance los 8,1 miles de millones de Introduction Corn (Zea mays L.) is one of the predominant crops in international markets, it is the staple food for more than 200 million people and it is estimated that this number will grow as the world population reaches 8.1 thousand million 575 Esta publicación científica en formato digital es continuación de la Revista Impresa: Depósito legal pp 196802ZU42, ISSN 0378-7818. Rev. Fac. Agron. (LUZ). 2021, 38(3): 573-584. Julio-Septiembre. Peña et al. ISSN 2477-9407 habitantes en 2025 (United Nation, 2017). Según Faure et al. (2014), en Cuba dentro de las producciones de cultivos varios, el maíz constituye una de las más importantes. Según el anuario estadístico de Cuba (ONE, 2016) se siembran anualmente 185.922 ha de maíz de estas 7.286 ha pertenecen al sector estatal y 178.636 ha al sector privado. La producción total obtenida en esta área es de 427.295 t de ellas 13.882 t de sector estatal y 413.413 t del privado. El rendimiento promedio es de 2,30 t.ha -1 entre los dos sectores, por lo que la producción es insuficiente e inversa a la demanda. El VIUSID agro ® pudiera ser una alternativa al incremento de la producción de maíz sin afectar el medio ambiente. En países como Cuba (Meléndrez et al. 2016) y Egipto (Atta et al., 2017, Abdel-Lattif et al., 2018;) se han reportados resultados satisfactorios con el uso de este producto. Sin embargo, aún las investigaciones son insuficientes partiendo de que las réplicas con diferentes especies, variedades y tratamientos en el tiempo, son un requisito en la investigación agropecuaria. Por lo que el objetivo de la investigación fue determinar el efecto de la aplicación foliar de dosis de VIUSID agro ® en el comportamiento agroproductivo del cultivo de maíz (Zea mays L.). Materiales y métodos Se realizaron dos experimentos en la Unidad Básica de Producción Cooperativa (UBPC) Sopimpa, Fomento, Sancti Spíritus, Cuba. La inhabitants in 2025 (United Nations, 2017). According to Faure et al. (2014), in Cuba within the productions of various crops, corn is one of the most important. According to the statistical yearbook of Cuba (ONE, 2016) 185,922 ha of corn are sown annually, of these 7,286 ha belong to state sector and 178,636 ha to private sector. The total production obtained in this area is 427,295 t, of which 13,882 t from state sector and 413,413 t from private sector. The average yield is 2.30 t.ha -1 between the two sectors, so production is insufficient and inverse to demand. VIUSID agro ® could be an alternative to increasing corn production without affecting the environment. In countries such as Cuba (Meléndrez et al. 2016) and Egypt (Atta et al., 2017, Abdel-Lattif et al., 2018;) satisfactory results have been reported with the use of this product. However, research is still insufficient, based on the fact that replications with different species, varieties and treatments over time are a requirement in agricultural research. Therefore, the objective of the research was to determine the effect of the foliar application of VIUSID agro ® doses on the agroproductive behavior of the corn (Zea mays L.) crop. Materials and methods Two experiments were carried out in the Unidad Básica de Producción Cooperativa (UBPC) Sopimpa, Fomento, Sancti Spíritus, Cuba. The variety of corn that was used was the 576 Esta publicación científica en formato digital es continuación de la Revista Impresa: Depósito legal pp 196802ZU42, ISSN 0378-7818. Rev. Fac. Agron. (LUZ). 2021, 38(3): 573-584. Julio-Septiembre. Peña et al. ISSN 2477-9407 variedad de maíz que se usó fue la “Criollo” y el tipo de suelo fue Fluvisol según la Base de Referencia Mundial para los Recursos de la Tierra, siglas en inglés, (WRB, 2014). La siembra del experimento 1 fue el 20 de abril de 2016 y la cosecha 122 días después de la siembra. Las variables climáticas durante el experimento fueron registradas por la Estación Provincial de Sancti Spíritus. La temperatura media diaria fue de 25,97 º C, la humedad relativa media diaria 77,36 % y la precipitación pluvial acumulada de 56,7 mm. La fecha de siembra del experimento 2 fue el 22 de mayo de 2017 y la cosecha 123 días después de la siembra. Las variables climáticas fueron: temperatura media diaria 25,97 º C, la humedad relativa media diaria 79,0 % y la precipitación pluvial acumulada de 71,12 mm. Ambos experimentos fueron plantados a inicios de la época lluviosa que es la etapa considerada óptima para las condiciones de Cuba. Las semillas para la siembra fueron proporcionadas por el propio productor y proveniente del año anterior. Para la selección del área, la preparación de suelo, siembra, riego y el control de plagas se siguieron las normas técnicas del cultivo del maíz (MINAG, 2017). El diseño experimental en ambos experimentos fue bloques al azar con 4 tratamientos y tres réplicas. Las parcelas fueron de 30 m 2 para un área experimental de 360 m 2 , la defensa interna por parcelas fue de 0,5 m 2 y el área de cálculo de 20 m 2 . Se evaluaron 10 plantas por parcelas, 30 por tratamiento, escogidas al azar en la superficie de cálculo. “Criollo” and the soil type was Fluvisol according to the World Reference Base for Land Resources, (WRB, 2014). The sowing of experiment 1 was on April 20, 2016 and the harvest 122 days after sowing. The climatic variables during the experiment were registered by the Estación Provincial de Sancti Spíritus. The average daily temperature was 25.97 ºC, the average daily relative humidity was 77.36 % and the accumulated rainfall was 56.7 mm. The sowing date of experiment 2 was May 22, 2017 and the harvest was 123 days after sowing. The climatic variables were: Average daily temperature 25.97 ºC, average daily relative humidity 79.0 % and accumulated rainfall of 71.12 mm. Both experiments were planted at the beginning of the rainy season, which is the stage considered optimal for Cuban conditions. The seeds for sowing were provided by the producer himself and from the previous year. For the selection of the area, soil preparation, planting, irrigation and pest control, the technical standards for corn cultivation were followed (MINAG, 2017). The experimental design in both experiments was randomized blocks with 4 treatments and three replications. The plots were 30 m 2 for an experimental area of 360 m 2 , the internal defense per plot was 0.5 m 2 and the calculation area was 20 m 2 . 10 plants were evaluated per plots, 30 per treatment, random chosen on the calculation surface. The foliar application in both experiments was in the morning 577 Esta publicación científica en formato digital es continuación de la Revista Impresa: Depósito legal pp 196802ZU42, ISSN 0378-7818. Rev. Fac. Agron. (LUZ). 2021, 38(3): 573-584. Julio-Septiembre. Peña et al. ISSN 2477-9407 La aplicación foliar en ambos experimentos fue en horas de la mañana, se usó un aspersor foliar de espalda con capacidad de 16 litros. Se realizaron tres aplicaciones a los 15, 30 y 45 días después de la germinación. Los tratamientos en ambos experimentos fueron: Un control, cero aplicaciones de VIUSID agro ® y 0,1; 0,3 y 0,5 L.ha - 1 VIUSID agro ® la composición del producto se encuentra detallada según (Peña et al., 2018c). Se realizaron dos evaluaciones de las variables y se tuvo en cuenta aspectos del descriptor varietal de maíz propuesto por el Centro Internacional de Agricultura Tropical (CIAT) (Muñoz et al., 2004). La primera evaluación fue a los 90 días después de la siembra. Las variables evaluadas fueron: longitud de la planta (cm), diámetro del tallo (cm), área foliar (cm 2 ). En el momento de la cosecha se evaluó: altura de inserción de la mazorca (cm), longitud de la mazorca (cm), diámetro de la mazorca (cm), masa de la mazorca (g), número de hileras por mazorca, número de granos por mazorca, masa de los granos por mazorca (g), masa de 1000 granos (g) y rendimiento agrícola (t.ha -1 ). Para la longitud de la planta se usó una cinta métrica y la planta se midió desde el cuello de la raíz hasta el ápice. El diámetro del tallo se midió a los 100 cm de longitud con la ayuda de un calibre o venier. La longitud de la mazorca y la altura de inserción de la misma, se determinó con el usó de la cinta métrica. Para el área foliar se midió el largo y ancho de las hojas y se calculó con la siguiente fórmula AF= (L x A) F donde AF es el área foliar, L es el largo de la hoja, A es el ancho y F es el factor según el hours, a back-mounted foliar sprinkler with a capacity of 16 liters was used. Three applications were made at 15, 30 and 45 days after germination. The treatments in both experiments were: A control, zero applications of VIUSID agro® and 0.1; 0.3 and 0.5 L.ha -1 VIUSID agro® the composition of the product is detailed according to Peña et al. (2018c). Two evaluations of the variables were carried out and aspects of the maize varietal descriptor proposed by the Centro Internacional de Agricultura Tropical (CIAT) (Muñoz et al., 2004) were taken into account. The first evaluation was 90 days after sowing. The variables evaluated were: plant length (cm), stem diameter (cm), leaf area (cm 2 ). At the time of harvest, the following were evaluated: ear insertion height (cm), ear length (cm), ear diameter (cm), ear mass (g), number of rows per ear, number of grains per ear, mass of grains per ear (g), mass of 1000 grains (g) and agricultural yield (t.ha -1 ). For length of the plant a tape measure was used and the plant was measured from the root neck to the apex. The diameter of the stem was measured at 100 cm in length with the help of a caliper or venier. The length of the ear and its height of insertion, was determined with the use of the measuring tape. For the leaf area, the length and width of the leaves were measured and it was calculated with the following formula AF = (L x A) F where AF is the leaf area, L is the length of the leaf, A is the width and F is the factor according to the crop. The number of rows and kernels 578 Esta publicación científica en formato digital es continuación de la Revista Impresa: Depósito legal pp 196802ZU42, ISSN 0378-7818. Rev. Fac. Agron. (LUZ). 2021, 38(3): 573-584. Julio-Septiembre. Peña et al. ISSN 2477-9407 cultivo. El número de hileras y de granos por mazorca fue contado manualmente. Con la balanza del tipo digital Sartorius, con una precisión de 0,01 g se determinó la masa de la mazorca y granos, el rendimiento se estimó a través del método indirecto. Los datos se procesaron con el paquete estadístico SPSS v. 15.1.0 para el Windows (SPSS, 2006) para Windows. Para la normalidad se realizó la prueba de Kolmogorov – Smirnov y la dócima de Levene para la homogeneidad. Cuando existió normalidad y homogeneidad se realizó un análisis de varianza de clasificación simple (ANOVA) y la prueba de rangos múltiples de Tukey cuando (p<0,05). La prueba de Kruskal – Wallis y prueba U de Mann – Whitney se aplicó cuando no existía normalidad de los datos. Resultados y discusión El ANOVA reveló diferencias significativas (p<0,05) entre los tratamientos en todas las variables evaluadas (cuadro 1). Se observó el efecto de los tratamientos en la LP a los 90 días después de la siembra (dds). El comportamiento más favorable en el experimento 1 fue alcanzado con el tratamiento 0,5 L.ha -1 de VIUSID agro ® el que difirió significativamente del control y del resto de los tratamientos con el producto. El incremento respecto al no tratado fue de 11,61 %. En el experimento 2 (cuadro 1) en la LP, todos los tratamientos con el VIUSID agro ® difirieron significativamente del control excepto la dosis menor. Obteniéndose los mayores valores para los tratamientos 0,3 y 0,5 L.ha -1 con incrementos de 10,08 y 16,47 % en comparación con el control. per ear was counted manually. With the Sartorius digital scale, with a precision of 0.01 g, the mass of the cob and grains was determined, the yield was estimated through the indirect method. Data were processed with the statistical package SPSS v. 15.1.0 for Windows (SPSS, 2006) for Windows. For normality, the Kolmogorov - Smirnov test and the Levene test for homogeneity were performed. When normality and homogeneity existed, a simple classification analysis of variance (ANOVA) was performed and the Tukey multiple range test when p <0.05. The Kruskal - Wallis test and the Mann - Whitney U test were applied when there was no normality of the data. Results and discussion The ANOVA revealed significant differences (p <0.05) between the treatments in all the variables evaluated (table 1). The effect of the treatments on the LP can be seen at 90 days after sowing (dds). The most favorable behavior in experiment 1 was achieved with the treatment 0.5 L.ha -1 of VIUSID agro®, which differed significantly from the control and from the rest of the treatments with the product. The increase compared to the untreated was 11.61 %. In experiment 2 (table 1) in the LP, all the treatments with VIUSID agro ® differed significantly from the control except the lower dose. Obtaining the highest values for the treatments 0.3 and 0.5 L.ha -1 with increments of 10.08 and 16.47 % compared to the control. 579 Esta publicación científica en formato digital es continuación de la Revista Impresa: Depósito legal pp 196802ZU42, ISSN 0378-7818. Rev. Fac. Agron. (LUZ). 2021, 38(3): 573-584. Julio-Septiembre. Peña et al. ISSN 2477-9407 Cuadro 1. Efecto de los tratamientos de VIUSID agro ® en la longitud de la planta, el diámetro del tallo, el área foliar, altura de la inserción de la mazorca, en el número de hileras por mazorca, granos por hilera, granos por mazorca, masa de los granos, masa de 1000 granos y el rendimiento agrícola. Unidad Básica de Producción Cooperativa (UBPC) Sopimpa, Fomento, Sancti Spíritus, Cuba. Table 1. Effect of VIUSID agro ® treatments on plant length, stem diameter, leaf area, ear insertion height, number of rows per ear, grains per row, grains per ear, mass of grains, mass of 1000 grains and agricultural yield. Unidad Básica de Producción Cooperativa (UBPC) Sopimpa, Fomento, Sancti Spiritus, Cuba. Tratamientos LP DT AF AI NH GM MG M1000 R (L.ha −1 ) (cm) (cm) (cm 2 ) (cm) - - (g) (g) (t.ha −1 ) Experimento 1 Control 259,63±2,49 b 1,17±0,002 b 803,00±8,62 a 163,87±2,73 b 14,47±0,25 b 487,40±11,77 c 151,54±5,64 c 310,76±8,33 c 5,61±0,23 c 0,1 270,13±2,76 b 1,37±0,002 a 779,00±7,59 a 167,13±2,57 ab 14,60±0,31 b 527,20±13,85 b 171,77±5,84 b 328,52±10,35 b 6,36±0,24 b 0,3 271,60±2,57 b 1,42±0,002 a 800,00±8,34 a 170,45±3,56 a 15,60±0,35 a 594,33±14,32 a 196,65±6,36 a 331,52±8,63 b 7,28±0,27 a 0,5 289,77±1,68 a 1,40±0,002 a 842,00±8,19 a 174,23±1,73 a 16,33±0,36 a 617,80±15,70 a 210,83±6,78 a 343,19±9,86 a 7,80±0,28 a CV(%) 5,01 7,22 11,75 9,03 12,39 16,53 22,15 15,77 22,21 Experimento 2 Control 182,17±1,84 c 1,11±0,001 c 529,90±9,40 b 85,00±1,09 b 13,20±0,32 a 422,74±13,01 b 155,30±5,87 c 368,29±10,14 b 4,19 ±0,15 c 0,1 193,63±2,31 bc 1,56±0,002 b 576,30±8,39 ab 91,93±1,55 b 12,80±0,28 a 453,37±14,07 b 159,13±3,65 c 358,15±10,85 b 4,30 ±0,09 c 0,3 200,53±3,08 ab 1,62±0,002 a 611,75±9.01 a 100,57±1,75 a 13,27±0,31 a 512,18±15,44 a 185,00±3,54 b 367,66±10,31 b 5,00 ±0,09 b 0,5 212,20±3,11 a 1,66±0,003 a 706,75±9,22 a 99,50±4,26 a 13,40±0,27 a 537,72±12,67 a 234,60±5,41 a 441,87±12,67 a 6,33 ±0,14 a CV (%) 9,13 16,78 17,01 15,80 11,93 18,23 22,23 17,87 22,17 Valores representados por las medias de tres réplicas ± Error estándar (E.E.). Medias con letras desiguales en la misma columna difieren para (P<0,05) según prueba de rangos múltiples de Tukey. LP: longitud de las plantas; DT: diámetro del tallo; LHD: longitud de la hoja bandera; AHB: ancho de la hoja bandera; AI: Altura de inserción de la mazorca; NH: número de hileras; GM: granos por mazorca; MG: masa de los granos; M1000: masa de mil granos; R: rendimiento agrícola. Values represented by the means of three replications ± Standard error (S.E.). Means with unequal letters in the same column differ for P <0.05 according to Tukey’s multiple range test. LP: length of the plants; DT: stem diameter; LHD: length of the flag sheet; AHB: width of the flag leaf; AI: Height of ear insertion; NH: number of rows; GM: grains per ear; MG: mass of the grains; M1000: mass of thousand grains; A: agricultural yield. 580 Esta publicación científica en formato digital es continuación de la Revista Impresa: Depósito legal pp 196802ZU42, ISSN 0378-7818. Rev. Fac. Agron. (LUZ). 2021, 38(3): 573-584. Julio-Septiembre. Peña et al. ISSN 2477-9407 Para el diámetro del tallo, en el experimento 1 cuadro 1, los comportamientos más favorables fueron de los tratamientos con VIUSID agro ® que no difirieron entre ellos, pero superaron significativamente al control en 17,09; 21,37 y 19,66 %. En el experimento 2 cuadro 1 el diámetro de tallo fue significativamente superior cuando se realizaron aplicaciones foliares de VIUSID agro ® . Los tratamientos de mejor comportamiento fueron las dosis 0,3 y 0,5 L.ha -1 de VIUSID agro ® con incrementos en relación al control de 45,95 y 49,55 %, respectivamente. En el área foliar solo se observaron diferencias estadísticas significativas en el experimento 2 para la dosis 0,5 L.ha -1 de VIUSID que difirió del control y lo superó en 33,74 %. En la altura de inserción de la mazorca (AI) solamente existieron diferencias significativas en el experimento 2 en los tratamientos con dosis de 0,3 y 0,5 L.ha -1 de VIUSID con un incremento en relación al control de un 18,32 y 17,06 % (cuadro 1). En el experimento 1, se observó que en el número de hileras (NH) los tratamientos de mejor comportamiento fueron las dosis 0,3 y 0,5 L.ha -1 de VIUSID y superaron al control en 1,13 y 1,86 hileras por mazorca lo que significó un incremento del 7,81 y 12,85 %, respectivamente. En la variable granos por mazorca (GM) en el experimento 1 y 2 el comportamiento más favorable fue de los tratamientos con VIUSID agro ® con dosis de 0,3 y 0,5 L.ha -1 con un incremento en relación al control de 21,94 y 26,75 %, experimento 1 y 21,16 For stem diameter, in experiment 1 table 1,, the most favorable behaviors were of the treatments with VIUSID agro ® that did not differ between them, but significantly exceeded the control in 17.09; 21.37 and 19.66 %. In experiment 2, table 1, the stem diameter was significantly higher when foliar applications of VIUSID agro ® were made. The treatments with the best performance were the doses 0.3 and 0.5 L.ha -1 of VIUSID agro® with increases in relation to the control of 45.95 and 49.55 %, respectively. In the foliar area, only significant statistical differences were observed in experiment 2 for the dose 0.5 L. ha -1 of VIUSID, which differed from the control and exceeded it by 33.74 %. In the height of insertion of the ear (AI) there were only significant differences in experiment 2 in the treatments with doses of 0.3 and 0.5 L. ha -1 of VIUSID with an increase in relation to the control of 18, 32 and 17.06 % (table 1). In experiment 1, it was observed that in the number of rows (NH) the treatments with the best performance were the doses 0.3 and 0.5 L.ha -1 of VIUSID and they surpassed the control in 1.13 and 1.86 rows per ear which meant an increase of 7.81 and 12.85 %, respectively. In the variable grains per ear (GM) in experiment 1 and 2 the most favorable behavior was of the treatments with VIUSID agro ® with doses of 0.3 and 0.5 L.ha -1 with an increase in relation to the control of 21.94 and 26.75 %, experiment 1 and 581 Esta publicación científica en formato digital es continuación de la Revista Impresa: Depósito legal pp 196802ZU42, ISSN 0378-7818. Rev. Fac. Agron. (LUZ). 2021, 38(3): 573-584. Julio-Septiembre. Peña et al. ISSN 2477-9407 y 27,20 % experimento 2. En la masa de los granos en ambos experimentos el comportamiento fue similar hubo un incremento significativo entre los tratamientos a medida que se incrementó la dosis de VIUSID agro ® en el experimento 1 fueron los tratamientos 0,3 y 0,5 L.ha -1 de VIUSID agro ® los de comportamiento más favorable con incrementos en relación al control de 29,77 y 39,12 %. En el experimento 2 fue de la dosis 0,5 L.ha -1 la que difirió del resto de los tratamientos para un incremento en la producción por planta del 51,06 % (cuadro 1). En la masa de 1000 granos en ambos (cuadro 1) el comportamientos más favorables fue con el tratamiento 0,5 L.ha -1 de VIUSID agro ® e incrementos respecto al control de 10,44 % experimento 1 y 19,98 %, experimento 2 (cuadro 1). En el rendimiento agrícola, en ambos experimentos los resultados más favorables se alcanzaron con las dosis 0,3 y 0,5 L.ha -1 de VIUSID agro ® para un aumento de la producción del 29,77 y 39,04 % en el experimento 1 y de 19,33 y 51,07 % en el experimento 2 En este estudio se demostró que el VIUSID agro ® desempeña un rol importante en el crecimiento de las plantas maíz. Estos efectos favorables pudieron estar atribuido a que actúa como biorregulador natural, debido a su composición (Peña et al., 2018c), que favorecen los procesos de germinación, floración, producción de frutos, metabolismo de los aminoácidos y en la síntesis de bases nitrogenadas requerida para la formación 21.16 and 27.20 % experiment 2. In the mass of the grains in both experiments the behavior was similar, there was a significant increase between the treatments as the dose was increased of VIUSID agro ® in experiment 1 were the treatments 0.3 and 0.5 L.ha -1 of VIUSID agro ® those with the most favorable behavior with increases in relation to the control of 29.77 and 39.12 %. In experiment 2, the dose of 0.5 L.ha -1 was the one that differed from the rest of the treatments for an increase in production per plant of 51.06 % (table 1). In the mass of 1000 grains in both (table 1) the most favorable behavior was with the treatment 0.5 L.ha -1 of VIUSID agro ® and increases compared to the control of 10.44 % experiment 1 and 19.98 %, experiment 2 (table 1). In agricultural yield, in both experiments the most favorable results were achieved with the doses 0.3 and 0.5 L.ha -1 of VIUSID agro ® for an increase in production of 29.77 and 39.04 % in the experiment 1 and 19.33 and 51.07 % in experiment 2. In this study it was shown that VIUSID agro ® plays an important role in the growth of corn plants. These favorable effects could be attributed to the fact that it acts as a natural bioregulator, due to its composition (Peña et al., 2018c), which favor germination, flowering, fruit production, amino acid metabolism and the synthesis of nitrogenous bases. required for the formation of new tissues. In addition, it is widely known that biostimulants play an important role, because they stimulate metabolic, physiological and 582 Esta publicación científica en formato digital es continuación de la Revista Impresa: Depósito legal pp 196802ZU42, ISSN 0378-7818. Rev. Fac. Agron. (LUZ). 2021, 38(3): 573-584. Julio-Septiembre. Peña et al. ISSN 2477-9407 de nuevos tejidos. Además, es ampliamente conocido que los bioestimulantes desempeñan un papel importante, porque estimulan procesos metabólicos, fisiológicos y bioquímicos que favorecen el crecimiento y desarrollo de las plantas (Yakhin et al., 2017). Por otra parte, con la investigación se verificaron los efectos bioestimulantes de la utilización del VIUSID agro ® en las plantas de maíz. Descubrimientos recientes han demostrado que este producto favorece el incremento de algunos indicadores en plantas de maíz, con la aplicación de dosis de 0,2 L.ha -1 cada 7 y 10 días (Atta et al., 2017, Abdel-Lattif et al., 2018), quienes incrementaron parámetros morfológicos con la aplicación de de este producto. Otros autores como (Meléndrez et al. 2016) recomendaron el uso de VIUSID agro para el incremento del rendimiento en maíz. Otras investigaciones han demostrado los efectos bioestimulantes de la aplicación foliar del VIUSID agro ® en plantas de anturio (Anturium andreanum Lind) (Peña et al., 2015a), frijol (Phaseolus vulgaris L.) (Peña et al., 2015a; 2015b; 2017b), cebolla (Allium cepa L.); lechuga (Lactuca sativa L.) y la acelga (Beta vulgaris var. Cicla) (Peña et al., 2017a), rábano (Raphanus sativus L.) (Peña et al., 2018a, 2018c)0.5, 0.7 y 1.0 L.ha-1 del promotor del crecimiento y un control. Dentro de los in-dicadores evaluados estuvieron la masa fresca y seca de la planta. Se evaluaron el diámetro polar y ecuatorial del tallo fresco, los índices biochemical processes that favor plant growth and development (Yakhin et al., 2017). On the other hand, the research verified the biostimulant effects of the use of VIUSID agro ® in corn plants. Recent discoveries have shown that this product favors the increase of some indicators in corn plants, with the application of doses of 0.2 L.ha -1 every 7 and 10 days (Atta et al., 2017, Abdel-Lattif et al., 2018), who increased morphological parameters with the application of this product. Other authors such as (Meléndrez et al. 2016) recommended the use of VIUSID agro ® to increase corn yield. Other investigations have demonstrated the biostimulant effects of the foliar application of VIUSID agro ® in anthurium plants (Anturium andreanum Lind) (Peña et al., 2015a), beans (Phaseolus vulgaris L.) (Peña et al., 2015a; 2015b; 2017b), onion (Allium cepa L.); lettuce (Lactuca sativa L.) and chard (Beta vulgaris var. Cicla) (Peña et al., 2017a), radish (Raphanus sativus L.) (Peña et al., 2018a, 2018c), beet (Beta vulgaris L. ) (Peña et al., 2017a), tobacco (Nicotiana tabacum L.) (Peña et al., 2018b) and Chinese cabbage (Brassica rapa subsp) (Peña et al., 2019). Conclusions The application of the VIUSID agro ® growth promoter improves the growth and development of corn plants, showing that as the dose of VIUSID agro ® is increased, the agroproductive behavior of the crop improves considerably. 583 Esta publicación científica en formato digital es continuación de la Revista Impresa: Depósito legal pp 196802ZU42, ISSN 0378-7818. Rev. Fac. Agron. (LUZ). 2021, 38(3): 573-584. Julio-Septiembre. Peña et al. ISSN 2477-9407 End of English Version de creci-miento fisiológicos, eficiencia foliar y productiva y rendimiento agrícola. Los resultados mostra-ron incrementos significativos (P≤0,05, remolacha (Beta vulgaris L.) (Peña et al., 2017a), tabaco (Nicotiana tabacum L.) (Peña et al., 2018b) y la col china (Brassica rapa subsp) (Peña et al., 2019). Conclusiones La aplicación del promotor del crecimiento VIUSID agro® mejora el crecimiento y desarrollo de las plantas de maíz, evidenciándose que a medida que se aumenta la dosis de VIUSID agro ® el comportamiento agroproductivo del cultivo mejora considerablemente. Los hallazgos de este estudio sugieren que el VIUSID agro ® desempeña un rol importante en plantas de maíz en condiciones de campo y constituye una alternativa eficiente para incrementar la producción de este cultivo. Literatura citada Abdel-Lattif, H.M., R.Absy and M.M. Atta. 2018. Effect of growth promoter supplement on yield and grain quality of maize (Zea mays L.). Egypt J. Agron. 40(2):165−180. Atta, M.M.M., H.M. Abdel−Lattif and R. Absy. 2017. 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