Cambios dinámicos en la homeostasis tiol–disulfuro y el estrés oxidativo durante el tratamiento con vincristina en perras Kangal con tumor venéreo transmisible

• Murat Can Demir Kafkas University, Faculty of Veterinary Medicine, Department of Obstetrics and Gynecology, 36100, Kars, Türkiye. https://orcid.org/0000-0002-3803-069X
• Mushap Kuru Kafkas University, Faculty of Veterinary Medicine, Department of Obstetrics and Gynecology, 36100, Kars, Türkiye. https://orcid.org/0000-0003-4409-251X
• Mustafa Makav Kafkas University, Faculty of Veterinary Medicine, Department of Physiology, 36100, Kars, Türkiye. https://orcid.org/0000-0003-1879-8180
• Emin Karakurt Kafkas University, Faculty of Veterinary Medicine, Department of Pathology, 36100, Kars, Türkiye. https://orcid.org/0000-0003-2019-3690
• Merve Sena Demir Kafkas University, Faculty of Veterinary Medicine, Department of Obstetrics and Gynecology, 36100, Kars, Türkiye. https://orcid.org/0000-0002-2630-8818
• Burak Büyükbaki Kafkas University, Faculty of Veterinary Medicine, Department of Wildlife and Ecology, 36100, Kars, Türkiye. https://orcid.org/0000-0002-4770-8220
• Semra Kaya Kafkas University, Faculty of Veterinary Medicine, Department of Obstetrics and Gynecology, 36100, Kars, Türkiye. https://orcid.org/0000-0002-7520-6631
• Cihan Kaçar Kafkas University, Faculty of Veterinary Medicine, Department of Obstetrics and Gynecology, 36100, Kars, Türkiye. https://orcid.org/0000-0002-2642-697X

DOI: https://doi.org/10.52973/rcfcv-e363969

Palabras clave: Tumores venéreos, glutatión, malondialdehído, estrés oxidativo, vincristina

Resumen

El objetivo de este estudio fue evaluar los cambios dependientes del tiempo en los biomarcadores de estrés oxidativo y la homeostasis tiol- disulfuro en perras diagnosticados con tumor venéreo transmisible canino y tratados con sulfato de vincristina. Dieciocho perras Kangal fueron incluidas en el estudio, nueve con tumor venéreo transmisible canino y nueve clínicamente sanas (grupo control). Las perras en el grupo tumor venéreo transmisible canino recibieron sulfato de vincristina una vez por semana hasta que se logró la regresión completa del tumor. Los análisis se realizaron en el pretratamiento, semana 1 (T1), semana 3 (T3) y final del tratamiento. Solo se tomó una muestra de las perras del grupo control. Se determinaron los niveles séricos de malondialdehído, glutatión, tiol total, tiol nativo, disulfuro y las relaciones tiol-disulfuro. En perros con tumor venéreo transmisible canino, los niveles de  malondialdehído  fueron significativamente más altos (P = 0,001) durante el período pretratamiento en comparación con el grupo control sano, mientras que los niveles de glutatión (P = 0,002), tiol total (P = 0,001) y tiol nativo (P = 0,001) fueron significativamente más bajos. Durante el mismo período, los niveles de disulfuro (P = 0,001), disulfuro/tiol total × 100 (P = 0,001) y las razones disulfuro/tiol nativo × 100 (P = 0,001) aumentaron significativamente, mientras que la razón tiol nativo/tiol total × 100 (P = 0,001) disminuyó significativamente. Aunque se observó una mejora gradual en los parámetros de estrés oxidativo y la homeostasis tiol-disulfuro durante el tratamiento con vincristina, los niveles de malondialdehído permanecieron significativamente más altos que el grupo control al final del tratamiento (P = 0,001), y la homeostasis tiol-disulfuro no volvió completamente a la normalidad. En conclusión, las perras Kangal con tumor venéreo transmisible canino presentan un marcado estado de estrés oxidativo junto con una alteración de la homeostasis tiol-disulfuro. El tratamiento con vincristina mejora parcialmente este desequilibrio redox, pero no logra una normalización completa. Los parámetros de la homeostasis tiol-disulfuro pueden reflejar cambios en el estado redox y ser indicadores útiles para evaluar los cambios bioquímicos relacionados con el tratamiento en la tumor venéreo transmisible canino.

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